Somatic chromosomes from intercalary meristems of grasses.

Sapre, A. B.; Barve, Siddhivinayak

doi:10.1508/cytologia.48.539

Cited by 4 publications

(4 citation statements)

References 9 publications

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“…The aquatica genome carries five large, more or less equal sized, pairs of chromosomes with median centromeres (Sapre & Barve, 1983). In contrast, in the gigantae genome the chromosomes are smaller and vary in size.…”

Section: Resultsmentioning

confidence: 99%

Isolation of a series of chromosomal variants (2n=10 to 21) from an open-pollinated population of interspecific hybrids between Coix gigantea and Coix aquatica (Poaceae)

Sapre

Deshpande

1987

Genetica

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A series of chromosomal variants has been isolated from an open-pollinated progeny of interspecific hybrids between aneuploids of Coix gigantea (2n = 18-24) and Coix aquatica (2n = 10). The interspecific hybrids (2n = 14, 15 and 16) produced several types of gametes not only with different chromosome numbers but also comprised of varied permutations and combinations ofgigantea and aquatica chromosomes. This was evident when the open-pollinated progeny obtained from these hybrids was screened chromosomally. Two such openpollinated experimental progenies were studied in two successive years (1983 and 1984) and plants with from 2n = 10 to 2n = 21 chromosomes were isolated. Chromosomal configurations at diakinesis in all the variants revealed frequent pairing between the gigantea and aquatica chromosomes. This indicated that the two species are phylogenetically closely related. Restoration of 'pure' parental species from the F1 hybids and chromosomal variants through genomic segregation and spontaneous back-cross are unique and noteworthy features.

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Section: Resultsmentioning

confidence: 99%

Isolation of a series of chromosomal variants (2n=10 to 21) from an open-pollinated population of interspecific hybrids between Coix gigantea and Coix aquatica (Poaceae)

Sapre

Deshpande

1987

Genetica

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“…Leaf meristem is another material for mitotic study. Sapre and Barve (1983) reported the usefulness of intercalary leaf meristem of grasses, i.e. Zea mays, Trilobachne cookie, and Coix aquatica, for high quality karyotype.…”

Section: Discussionmentioning

confidence: 99%

Chromosome Count on Young Anther of Banana Male Bud Using Ezymatic Maceration and Dapi Staining in Slide Preparation

Ahmad

Poerba

2020

BERITABIOLOGI

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Chromosome counting is the basis in describing the chromosomes number of organism that might useful for genetic study and classification. In banana studies, the root tip with a combination of non-fluorescent staining methods such as carmine or orcein and squash is the most common material for chromosome counting. In this study, we presented the usefulness of young anther of banana male bud with enzymatic maceration method for cell spreading and 4,6-diamino-2-phenyl-indole (DAPI) for staining agent to get a satisfying chromosomes image at metaphase for mitotic study of diploid and tetraploid bananas. The principle of this study is fixation using ethanol:acetic acid (3:1), enzymatic digestion, maceration and staining using DAPI. Our result showed that this method can provide well spread cells with intensely contrast of chromosomes images that satisfying for chromosome counting.

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“…Garber (1950) and Celarier (1956) had already proposed that five was the basic chromosome number of the tribe Andropogoneae (Panicoideae). By 2000, Watson & Dallwitz reported a basic number of 5 for the panicoid genera Andropogon L., Coix L., Cymbopogon Spreng., Elionurus Kunth ex Willd., Sorghum, Thelopogon, and Zea L. (based on studies by e.g., Harlan & al., 1952;Sisodia, 1970;Dujardin, 1978a;Wu, 1978;Sapre & Barve, 1983;Molina & Naranjo, 1986Lavania, 1987;Sapre & Deshpande, 1987;Morakinyo & Olorode, 1988;Spies & al., 1991;Rao & Nirmala, 1994).…”

Section: Discussionmentioning

confidence: 99%

Chromosome studies in the grass subfamily Chloridoideae. I. Basic chromosome numbers

Roodt

Spies

2003

TAXON

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Two main basic chromosome numbers occur in the majority of southern African Chloridoideae, i.e., 9 and 10, the majority being x = 10. Two main evolutionary pathways, one following x = 10 and the other following an aneuploid/dysploid reduction to x = 9 are suggested for the subfamily. Evidence indicates that x = 9, 10 are paleopolyploid basic chromosome numbers, with x = 10 derived from x = 5. This corresponds to the closely related Panicoideae where five is accepted as the basic chromosome number.

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Somatic chromosomes from intercalary meristems of grasses.

Cited by 4 publications

References 9 publications

Isolation of a series of chromosomal variants (2n=10 to 21) from an open-pollinated population of interspecific hybrids between Coix gigantea and Coix aquatica (Poaceae)

Isolation of a series of chromosomal variants (2n=10 to 21) from an open-pollinated population of interspecific hybrids between Coix gigantea and Coix aquatica (Poaceae)

Chromosome Count on Young Anther of Banana Male Bud Using Ezymatic Maceration and Dapi Staining in Slide Preparation

Chromosome studies in the grass subfamily Chloridoideae. I. Basic chromosome numbers

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