Solution structure and function of a conserved protein SP14.3 encoded by an essential Streptococcus pneumoniae gene 1 1Edited by M. F. Summers

Yu, Liping; Gunasekera, Angelo; Mack, J.; Olejniczak, Edward T.; Chovan, Linda E.; Ruan, Xiaoan; Towne, Danli L.; Lerner, Claude G.; Fesik, Stephen W.

doi:10.1006/jmbi.2001.4894

Cited by 23 publications

(24 citation statements)

References 59 publications

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“…The orthologue SP14.3 in S. pneumoniae was suggested to be essential based on the inability to insert a plasmid carrying a 300-bp internal 5′ portion of the gene into the chromosomal copy of the gene by homologous recombination. 61 However, since no measures seem to have been taken to avoid polar effects of the inserted plasmid on the expression of downstream chromosomal genes, we find it questionable whether or not SP14.3 is essential in S. pneumoniae. Immediately downstream from the gene for SP14.3 is the nusA gene, which is essential in E. coli.…”

Section: Discussionmentioning

confidence: 81%

“…53 The structure in solution of the RimP orthologue, SP14.3, in Streptococcus pneumoniae has been solved. 61 It was reported that SP14.3 contains two domains, both of which seem to resemble the folds of ribosomal proteins. The tertiary structure of the Nterminal domain is similar to ribosomal protein S3 of T. thermophilus, and the C-terminal domain has a protein fold resembling ribosomal protein L21E of Haloarcula marismortui.…”

Section: Discussionmentioning

confidence: 99%

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The RimP Protein Is Important for Maturation of the 30S Ribosomal Subunit

Nord

Bylund

Lövgren

et al. 2009

Journal of Molecular Biology

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

The RimP Protein Is Important for Maturation of the 30S Ribosomal Subunit

Nord

Bylund

Lövgren

et al. 2009

Journal of Molecular Biology

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“…A transformation-competent, macrolide-susceptible strain of S. pneumoniae (CP1250) (14) was used as the recipient strain. Transforming PCR product (0.1 to 1 g) was added to culture aliquots as described previously (20). The transformants were selected on THYE agar plates containing erythromycin.…”

Section: Methodsmentioning

confidence: 99%

Characterization and Prevalence of MefA, MefE, and the Associated msr (D) Gene in Streptococcus pneumoniae Clinical Isolates

et al. 2004

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Recent work has shown that the efflux genes in Streptococcus pneumoniae that are responsible for acquired macrolide resistance can be distinguished as either mef(E) or mef(A). The genetic elements on which mef(A) and mef(E) are found also carry an open reading frame (ORF) that is 56% homologous to msr(A) in Staphylococcus. The prevalence of mef(A/E) and of the msr-like ORF [msr(D)] was evaluated in 153 mef ؉ S. pneumoniae clinical isolates collected in North America, Europe, Africa, and Asia from 1997 to 2002. Clinical isolates were screened with PCR primers specific for either mef(A) or mef(E) and for msr(D). mef(A), mef(E), and msr(D) were cloned from mef ؉ strains and transformed into a susceptible, competent strain of S. pneumoniae. The transformants were tested for antimicrobial susceptibilities and efflux pump induction. The results of this work demonstrated that mef(A) is more often isolated in parts of Europe, with some incidence in Canada, and that the msr-like gene alone can confer the efflux phenotype.Recent work has shown that the efflux genes in Streptococcus pneumoniae responsible for acquired macrolide resistance can be distinguished as either mef(E) or mef(A) (6). Originally, mef in S. pneumoniae had been labeled mef(E), while mef(A) had been reserved for Streptococcus pyogenes. The two mef genes show a 90% sequence homology between the start and stop codons, but they can be distinguished with specific primer sets. Due to sequence similarity, these genes were merged under mef(A) by Roberts et al. (16). However, for clarity in the present discussion, the genes will be referred to as mef(A) and mef(E). Whether there are sufficient differences in the epidemiology and/or function of the genes to return to separate designations has not been determined.The mef genes are carried on transposons comprised of additional open reading frames (ORFs). Both of these genetic elements also carry an ORF downstream from mef that is 56% homologous to the coding region of msr(A) in Staphylococcus. The upstream region of the msr-like gene in Streptococcus lacks the leader peptide found in the Staphylococcus msr(A) gene (17). The msr-like homologs found associated with either mef(A) or mef(E) have 98% sequence homology. Although the msr-like homolog is believed to be a part of the efflux system, it has not been previously studied independently in Streptococcus.mef(A) in S. pneumoniae has been previously described in Italy (6). Given our worldwide clinical isolate collection, we studied the prevalence rates of mef(A) versus that of mef(E) in S. pneumoniae isolates collected in Europe, Asia, and North and South America. The prevalence and geographic distributions of mef(A) versus mef(E) in 153 clinical isolates of mef ϩ S. pneumoniae from six regions of the world were evaluated in this study. The prevalence and function of the msr homolog were also evaluated. This gene has been given the designation msr(D) (M. Roberts, personal communication) and was shown to be capable of independent function when cloned and expressed i...

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“…BlastP analysis aligned the spy1725 gene product, which has homologs in all sequenced streptococcal genomes, with the SP14.3 protein from S. pneumoniae [52] (80% sequence similarity; 67% identity). Based on structural characterization, SP14.3 is a predicted RNA-binding protein.…”

Section: Resultsmentioning

confidence: 99%

Novel Algorithms Reveal Streptococcal Transcriptomes and Clues about Undefined Genes

et al. 2007

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Bacteria–host interactions are dynamic processes, and understanding transcriptional responses that directly or indirectly regulate the expression of genes involved in initial infection stages would illuminate the molecular events that result in host colonization. We used oligonucleotide microarrays to monitor (in vitro) differential gene expression in group A streptococci during pharyngeal cell adherence, the first overt infection stage. We present neighbor clustering, a new computational method for further analyzing bacterial microarray data that combines two informative characteristics of bacterial genes that share common function or regulation: (1) similar gene expression profiles (i.e., co-expression); and (2) physical proximity of genes on the chromosome. This method identifies statistically significant clusters of co-expressed gene neighbors that potentially share common function or regulation by coupling statistically analyzed gene expression profiles with the chromosomal position of genes. We applied this method to our own data and to those of others, and we show that it identified a greater number of differentially expressed genes, facilitating the reconstruction of more multimeric proteins and complete metabolic pathways than would have been possible without its application. We assessed the biological significance of two identified genes by assaying deletion mutants for adherence in vitro and show that neighbor clustering indeed provides biologically relevant data. Neighbor clustering provides a more comprehensive view of the molecular responses of streptococci during pharyngeal cell adherence.

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Solution structure and function of a conserved protein SP14.3 encoded by an essential Streptococcus pneumoniae gene 1 1Edited by M. F. Summers

Cited by 23 publications

References 59 publications

The RimP Protein Is Important for Maturation of the 30S Ribosomal Subunit

The RimP Protein Is Important for Maturation of the 30S Ribosomal Subunit

Characterization and Prevalence of MefA, MefE, and the Associated msr (D) Gene in Streptococcus pneumoniae Clinical Isolates

Novel Algorithms Reveal Streptococcal Transcriptomes and Clues about Undefined Genes

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