Solution scattering structural analysis of the 70 S Escherichia coli ribosome by contrast variation. I. invariants and validation of electron microscopy models 1 1Edited by M. F. Moody

Contrast analysis of the composition of ribosomes extracted with different purification procedures

“…proteins and rRNAs are uniformly distributed along this plane. This result is con®rmed by a recent SANS analysis by Svergun et al (1997) on E. coli ribosomal samples containing selectively deuterated sub-components of the 70S complex.…”

Contrast analysis of the composition of ribosomes extracted with different purification procedures

“…proteins and rRNAs are uniformly distributed along this plane. This result is con®rmed by a recent SANS analysis by Svergun et al (1997) on E. coli ribosomal samples containing selectively deuterated sub-components of the 70S complex.…”

Solution scattering structural analysis of the 70 s Escherichia coli ribosome by contrast variation. II † . A model of the ribosome and its RNA at 3.5 nm resolution 1 †Paper I in this series is the accompanying paper, Svergun et al. (1997) 1Edited by M. F. Moody

“…The envelope functions that are used to model the shape of the components can obviously not represent cavities in the structure of the subunits. This solid body representation is, however, justi®ed by the model-independent experimental evidence (see Figures 4 and 5(b) and Conclusions of the accompanying paper, Svergun et al, 1997), indicating that the existence of an extended system of channels inside the subunits is very improbable. Moreover, as the optimized model is qualitatively in a good agreement with the outer contours of Stark's model, the channels and holes seem to be the main reason for the poor ®t to the experimental neutron scattering data produced by the latter.…”

Isolation and Physiochemical Properties of Protein-Rich Nematode Mitochondrial Ribosomes

“…Dynamic light scattering experiments were carried out with the sucrose density gradient purified 55 S mitoribosomes. To validate the techniques employed to characterize the mitoribosomes, we applied the standard procedures to the characterization of the physiochemical properties of the well-described 70 S E. coli ribosomes ( , ). Experiments were performed independently three times and similar results were obtained.…”

“…Northern hybridization analysis using agarose gel and DNA probes for the rRNA detection suggested that each of the purified peak fractions included the large subunit rRNA of an appropriate size, although it degraded easily during RNA extraction (data not shown). the mitoribosomes, we applied the standard procedures to the characterization of the physiochemical properties of the well-described 70 S E. coli ribosomes (39,40). Experiments were performed independently three times and similar results were obtained.…”

Contrast analysis of the composition of ribosomes extracted with different purification procedures

“…proteins and rRNAs are uniformly distributed along this plane. This result is con®rmed by a recent SANS analysis by Svergun et al (1997) on E. coli ribosomal samples containing selectively deuterated sub-components of the 70S complex.…”

Solution scattering structural analysis of the 70 s Escherichia coli ribosome by contrast variation. II † . A model of the ribosome and its RNA at 3.5 nm resolution 1 †Paper I in this series is the accompanying paper, Svergun et al. (1997) 1Edited by M. F. Moody

“…The envelope functions that are used to model the shape of the components can obviously not represent cavities in the structure of the subunits. This solid body representation is, however, justi®ed by the model-independent experimental evidence (see Figures 4 and 5(b) and Conclusions of the accompanying paper, Svergun et al, 1997), indicating that the existence of an extended system of channels inside the subunits is very improbable. Moreover, as the optimized model is qualitatively in a good agreement with the outer contours of Stark's model, the channels and holes seem to be the main reason for the poor ®t to the experimental neutron scattering data produced by the latter.…”

Isolation and Physiochemical Properties of Protein-Rich Nematode Mitochondrial Ribosomes

“…Dynamic light scattering experiments were carried out with the sucrose density gradient purified 55 S mitoribosomes. To validate the techniques employed to characterize the mitoribosomes, we applied the standard procedures to the characterization of the physiochemical properties of the well-described 70 S E. coli ribosomes ( , ). Experiments were performed independently three times and similar results were obtained.…”

“…Northern hybridization analysis using agarose gel and DNA probes for the rRNA detection suggested that each of the purified peak fractions included the large subunit rRNA of an appropriate size, although it degraded easily during RNA extraction (data not shown). the mitoribosomes, we applied the standard procedures to the characterization of the physiochemical properties of the well-described 70 S E. coli ribosomes (39,40). Experiments were performed independently three times and similar results were obtained.…”

Contrast analysis of the composition of ribosomes extracted with different purification procedures

“…proteins and rRNAs are uniformly distributed along this plane. This result is con®rmed by a recent SANS analysis by Svergun et al (1997) on E. coli ribosomal samples containing selectively deuterated sub-components of the 70S complex.…”

Solution scattering structural analysis of the 70 s Escherichia coli ribosome by contrast variation. II † . A model of the ribosome and its RNA at 3.5 nm resolution 1 †Paper I in this series is the accompanying paper, Svergun et al. (1997) 1Edited by M. F. Moody

“…The envelope functions that are used to model the shape of the components can obviously not represent cavities in the structure of the subunits. This solid body representation is, however, justi®ed by the model-independent experimental evidence (see Figures 4 and 5(b) and Conclusions of the accompanying paper, Svergun et al, 1997), indicating that the existence of an extended system of channels inside the subunits is very improbable. Moreover, as the optimized model is qualitatively in a good agreement with the outer contours of Stark's model, the channels and holes seem to be the main reason for the poor ®t to the experimental neutron scattering data produced by the latter.…”

Isolation and Physiochemical Properties of Protein-Rich Nematode Mitochondrial Ribosomes

“…Dynamic light scattering experiments were carried out with the sucrose density gradient purified 55 S mitoribosomes. To validate the techniques employed to characterize the mitoribosomes, we applied the standard procedures to the characterization of the physiochemical properties of the well-described 70 S E. coli ribosomes ( , ). Experiments were performed independently three times and similar results were obtained.…”

“…Northern hybridization analysis using agarose gel and DNA probes for the rRNA detection suggested that each of the purified peak fractions included the large subunit rRNA of an appropriate size, although it degraded easily during RNA extraction (data not shown). the mitoribosomes, we applied the standard procedures to the characterization of the physiochemical properties of the well-described 70 S E. coli ribosomes (39,40). Experiments were performed independently three times and similar results were obtained.…”