Solid-state cultivation of Bacillus thuringiensis R 176 with shrimp shells and rice straw as a substrate for chitinase production

Chaiharn, Mathurot; Lumyong, Saisamorn; Hasan, Najmul; Plikomol, Abhinya

doi:10.1007/s13213-012-0488-6

Cited by 17 publications

(3 citation statements)

References 22 publications

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“…The effects of different nitrogen sources revealed that inorganic nitrogen sources were the best for chitinase production by isolate B26. The same findings were found in B. thuringiensis 33 and Cohnella sp. A01.…”

Section: Discussionsupporting

confidence: 86%

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Antifungal Chitinase Production by Bacillus paramycoides B26 using Squid Pen Powder as a Carbon Source

Kurniawan¹,

Leelakriangsak²,

Panphon³

2022

J. Pure Appl. Microbiol.

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This study aimed to optimize the medium compositions and cultural conditions for improved chitinase production by a potential strain of Bacillus isolated from the marine environment and determine the antifungal activity of its chitinase against plant pathogenic fungi. Five potential isolates were cultured for chitinase production by submerged fermentation using colloidal chitin in a liquid medium. In this study, chitinase activity was determined by measuring reducing sugars, which were determined by the 3,5-dinitrosalicylic acid (DNS) assay. The most potential isolate, B26, showed similarity to Bacillus paramycoides based on the 16S rRNA gene sequence. The maximum chitinase production was achieved at 6.52±0.02 U/mL after 72 h of incubation in a medium containing 2% squid pen powder, supplemented with 0.5% sodium nitrate and 2% NaCl, with an initial pH of 7. It was observed that the optimization of cultural conditions resulted in 2.83 times higher chitinase production than an unoptimized medium. The antifungal activity of crude chitinase against phytopathogenic fungi was evaluated by a well-diffusion method. The chitinase of B. paramycoides B26 effectively inhibited the growth of Fusarium solani TISTR 3436 (83.4%) and Penicillium chrysogenum TISTR 3554 (80.12%).

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Section: Discussionsupporting

confidence: 86%

“…A similar result was also found in B. thuringiensis R176. 33 Another study showed that lactose and maltose decreased chitinase production, while chitin gave the highest enzyme production. 34 Isolate B26 produced the maximum chitinase when cultured in a medium containing the sole substrate.…”

Section: Discussionmentioning

confidence: 99%

Antifungal Chitinase Production by Bacillus paramycoides B26 using Squid Pen Powder as a Carbon Source

Kurniawan¹,

Leelakriangsak²,

Panphon³

2022

J. Pure Appl. Microbiol.

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“…While solid-state cultivation of B. thuringiensis R 176 with shrimp shells and rice straw as a substrate for chitinase production was studied, the optimal medium contained 0.5 % (w/v) ball-milled chitin (Chaiharn et al 2013 ). Thus, the present findings emphasize that chitinase production could be correlated with colloidal chitin concentration in the medium.…”

Section: Resultsmentioning

confidence: 99%

Biosynthesis and characterization of a thermostable, alkali-tolerant chitinase from Bacillus pumilus JUBCH08 displaying antagonism against phytopathogenic Fusarium oxysporum

et al. 2016

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The present investigation highlights the process parameters influencing the submerged fermentation of chitinase by Bacillus pumilus JUBCH08, purification and characterization of the enzyme and determination of antagonistic activity of the bacterium against Fusarium oxysporum. Medium supplemented with 0.5 % chitin and peptone, at initial pH 8.0, when incubated at 35 °C for 72 h favored highest chitinase production. The enzyme was purified 25.1-fold to homogeneity. The chitinase was found to be thermostable and alkali-tolerant with maximum activity at pH 8.0 and 70 °C for 1 h. The molecular weight of chitinase was found to be 64 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Mg2+, Co2+, Ca2+ and Mn2+ improved the chitinase activity. The K m and V max values of the enzyme were 0.13 mg/ml and 38.23 U/ml, respectively. When subjected to dual plate assay, the bacterium showed 45 % antagonism against F. oxysporum. Thus, it could be inferred that cultural conditions strongly affected the chitinase production by B. pumilus JUBCH08. The enzyme being thermostable and best functional under alkaline conditions could be useful for the feed industry and related biotechnological applications. Inhibition of F. oxysporum by the culture through lytic mechanism indicates its potentiality as a biocontrol agent.

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Shells and Other Calcium Carbonate-Based Waste

Baláž

2021

Environmental Mechanochemistry

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Solid-state cultivation of Bacillus thuringiensis R 176 with shrimp shells and rice straw as a substrate for chitinase production

Cited by 17 publications

References 22 publications

Antifungal Chitinase Production by Bacillus paramycoides B26 using Squid Pen Powder as a Carbon Source

Antifungal Chitinase Production by Bacillus paramycoides B26 using Squid Pen Powder as a Carbon Source

Biosynthesis and characterization of a thermostable, alkali-tolerant chitinase from Bacillus pumilus JUBCH08 displaying antagonism against phytopathogenic Fusarium oxysporum

Shells and Other Calcium Carbonate-Based Waste

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