Solid-phase microextraction set-up for the analysis of liver volatolome to detect livestock exposure to micropollutants

Bouhlel, Jihene; Ratel, Jérémy; Abouelkaram, S.; Mercier, Frédéric; Travel, Angélique; Baéza, Élisabeth; Jondreville, Catherine; Marchand, Philippe; Bizec, Bruno Le; Dubreil, Estelle; Mompelat, Sophie; Verdon, Eric; Inthavong, Chanthadary; Guérin, Thierry; Rutledge, Douglas N.; Engel, Erwan

doi:10.1016/j.chroma.2017.03.008

Cited by 13 publications

(16 citation statements)

References 48 publications

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“…The volatile compounds in the samples were analyzed via solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS) as previously described ( Bouhlel et al, 2017 ). Briefly, an automated sampler (MPS2, Gerstel) was used to conduct the following successive steps: (i) the sample was preheated in the agitator (500 rpm) for 10 min at 40°C, (ii) the volatile compounds were trapped by SPME (75 μm carboxen-polydimethylsiloxane, 23 gauge needle, Supelco) for 30 min at 40°C, and (iii) thermal desorption was performed at 280°C for 2 min in splitless mode in the GC inlet.…”

Section: Methodsmentioning

confidence: 99%

“…A volatile compounds analysis was performed by GC-full scan MS (GC6890, MS5973N, Agilent). The volatile compounds were separated on a RTX-5MS column (60 m × 0.32 mm × 1 μm, Restek) according to previously established settings ( Bouhlel et al, 2017 ). The volatiles were tentatively identified according to a comparison between their mass spectra and the NIST 14 mass spectral library and between published retention indices (RI) values and the RI values of an internal databank.…”

Section: Methodsmentioning

confidence: 99%

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Environmental Pollutant Benzo[a]Pyrene Impacts the Volatile Metabolome and Transcriptome of the Human Gut Microbiota

Defois

Ratel²,

Denis

et al. 2017

Front. Microbiol.

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Benzo[a]pyrene (B[a]P) is a ubiquitous, persistent, and carcinogenic pollutant that belongs to the large family of polycyclic aromatic hydrocarbons. Population exposure primarily occurs via contaminated food products, which introduces the pollutant to the digestive tract. Although the metabolism of B[a]P by host cells is well known, its impacts on the human gut microbiota, which plays a key role in health and disease, remain unexplored. We performed an in vitro assay using 16S barcoding, metatranscriptomics and volatile metabolomics to study the impact of B[a]P on two distinct human fecal microbiota. B[a]P exposure did not induce a significant change in the microbial structure; however, it altered the microbial volatolome in a dose-dependent manner. The transcript levels related to several metabolic pathways, such as vitamin and cofactor metabolism, cell wall compound metabolism, DNA repair and replication systems, and aromatic compound metabolism, were upregulated, whereas the transcript levels related to the glycolysis-gluconeogenesis pathway and bacterial chemotaxis toward simple carbohydrates were downregulated. These primary findings show that food pollutants, such as B[a]P, alter human gut microbiota activity. The observed shift in the volatolome demonstrates that B[a]P induces a specific deviation in the microbial metabolism.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Environmental Pollutant Benzo[a]Pyrene Impacts the Volatile Metabolome and Transcriptome of the Human Gut Microbiota

Defois

Ratel²,

Denis

et al. 2017

Front. Microbiol.

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“…Indeed, the past ten years have seen a number of very interesting studies based on modeling and on emerging analytical approaches (analytical chemistry, toxicogenomics, etc. ), particularly in the field of chemical safety for meat products [64][65][66][67][68][69][70]. These studies focused on the whole meat chain, from livestock exposure to micropollutants during farming [67] to meat digestion in the human gut [68,71].…”

Section: Example Of the Meat Sectormentioning

confidence: 99%

“…), particularly in the field of chemical safety for meat products [64][65][66][67][68][69][70]. These studies focused on the whole meat chain, from livestock exposure to micropollutants during farming [67] to meat digestion in the human gut [68,71]. In terms of processes applied to meat, several papers have demonstrated the relevance of combining analytical techniques, such as multi-dimensional gas chromatography, olfactometry, and mass spectrometry, to investigate both the heat-induced toxicants and odor-active compounds formed when cooking meat [69,70].…”

Section: Example Of the Meat Sectormentioning

confidence: 99%

Multi-Criteria Reverse Engineering for Food: Genesis and Ongoing Advances

et al. 2019

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Multi-criteria reverse engineering (MRE) has arisen from the cross-fertilization of advances in mathematics and shifts in social demand. MRE, thus, marks a progressive switch (a) from empirical to formal approaches able to simultaneously factor in diverse parameters, such as environment, economics, and health; (b) from mono-criterion optimization to multi-criteria decision analysis; (c) from forward engineering, observing the results of process conditions, to reverse engineering, selecting the right process conditions for a target output. The food sector has been slow to adopt reverse engineering, but interest is surging now that the industry is looking to shift production towards personalized food. MRE has followed a heterogeneous development trajectory and found applications in different disciplines. The scope of this review spans MRE applications in the food sector covering food packaging and food consumption and focuses on demonstrating potentialities of MRE in a complex field like food. We explain how MRE enables the development of sustainable processes, looking at similar approaches used in sectors other than food. Building on this extensive review, we sketch out some guidelines on approaches to be used in future MRE applications in food, working up from the problem statement.

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“…Zhao, She, et al (2017) reproducibility and recovery were obtained for lake water, rural water and farm water samples. Bouhlel et al (2017) investigated the extraction conditions of the solid-phase microextraction (SPME) process for the determination of volatile organic compounds (VOCs) from livestock. The research team identified 22 candidate VOC markers including some pesticides (e.g.…”

mentioning

confidence: 99%

Analytical Methods for Pesticides and Herbicides

Liu

Guo

Xue

2018

Water Environment Research

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A review of the literatures published in 2017 on topics relating to analytical methods for pesticides and herbicides is presented in this paper. Based on different techniques, this review is divided into seven parts, i. e., extraction methods, chromatographic or mass spectrometric techniques, electrochemical techniques, spectrophotometric techniques, chemiluminescence and fluorescence methods, biochemical assays and immunoassays, and miscellaneous techniques.

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Solid-phase microextraction set-up for the analysis of liver volatolome to detect livestock exposure to micropollutants

Cited by 13 publications

References 48 publications

Environmental Pollutant Benzo[a]Pyrene Impacts the Volatile Metabolome and Transcriptome of the Human Gut Microbiota

Environmental Pollutant Benzo[a]Pyrene Impacts the Volatile Metabolome and Transcriptome of the Human Gut Microbiota

Multi-Criteria Reverse Engineering for Food: Genesis and Ongoing Advances

Analytical Methods for Pesticides and Herbicides

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