Solid-phase extraction of DNA by using a composite prepared from multiwalled carbon nanotubes, chitosan, Fe3O4 and a poly(ethylene glycol)-based deep eutectic solvent

Ionic magnetic core–shell nanoparticles for DNA extraction

“…The analysis was conducted at room temperature in the eld of AE10 kOe. Structural identities are based on NMR spectra ( 1 H and 13 C, recorded on a Bruker AVN-400 MHz spectrometer).…”

“…Silyl chloride-functionalized magnetic nanoparticles (2.0 g) were dispersed in MeCN (50 mL) and ultrasonicated for 20 min. Alkyl imidazole (2.0 g) [13][14] was added and the reaction mixture was stirrer at 55 C for 2 days. Aer cooling to room temperature the product was isolated by magnetic decantation.…”

“…8,9 There have been a number of recent studies demonstrating the potential of magnetic nanoparticale as a mean of extraction or delivery of bio-molecules. [10][11][12][13][14][15][16][17][18][19] Lee et al systematically investigated an ultrasound enhanced siRNA delivery using a nanocomposite comprising of a deoxycholic acid-chitosane coated per-uoroakane droplets with attached magnetic nanoparticles. The exposure to ultrasound did not affect the siRNA-nanocomposite complex, which was found to be stable at 37 C for up to 4 h in the serum.…”

“…CH); 6.76 (bs, CH); 3.71 (d, CH 2 ); 1.66-1.60 (p, b-CH 2 ); 1.15 (mc, 16H, bulk-CH 2 ); 0.78 (t, 6H, CH 3 ) 13. C NMR (CDCl 3 ) d ¼ 137.36 (CHN 2 ), 128.97, 119.44 (CH), 50.62 (CH 2 N), 39.38 (CH), 31.66, (u-2), 31.15, 30.81, 29.47 (bulk-CH 2 ), 28.49 (g-CH 2 ), 26.29, (b-CH), 22.83, 22.58 (u-1), 14.00, 13.90 (CH 3 ).…”

Extraction of DNA from complex biological sample matrices using guanidinium ionic liquid modified magnetic nanocomposites

“…Highly accurate and reliable DNA analysis is the foundation of various biological applications. [1][2][3] However, separating DNA with high purity from the cell matrix or from biological samples in a complex environment is proven to be extremely complicated and challenging. 4,5 Proteins, metal ions, etc.…”