Solely aqueous formulation of hydrophobic cationic polymers for efficient gene delivery

Solomun, Jana I.; Cinar, Gizem; Mapfumo, Prosper; Richter, Friederike; Moek, Elisabeth; Hausig, Franziska; Martin, Liam; Hoeppener, Stephanie; Nischang, Ivo; Traeger, Anja

doi:10.1016/j.ijpharm.2020.120080

Cited by 26 publications

(33 citation statements)

References 61 publications

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“…The reduced uptake of [E100-PLGA](Chol) NP indicates an ATP-dependent uptake, such as endocytosis. It is known E100 particles [30] and particle blends of PLGA and cationic Eudragit [31] offer improved cellular uptake, supporting our data. Hence, the uptake and endosomal release of the drug were enhanced by co-formulated Eudragit and PLGA.…”

Section: Mechanism Of Protection From Pneumolysin By Nanoformulated Cholesterolsupporting

confidence: 88%

Intracellularly Released Cholesterol from Polymer-Based Delivery Systems Alters Cellular Responses to Pneumolysin and Promotes Cell Survival

et al. 2021

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Cholesterol is highly abundant within all human body cells and modulates critical cellular functions related to cellular plasticity, metabolism, and survival. The cholesterol-binding toxin pneumolysin represents an essential virulence factor of Streptococcus pneumoniae in establishing pneumonia and other pneumococcal infections. Thus, cholesterol scavenging of pneumolysin is a promising strategy to reduce S. pneumoniae induced lung damage. There may also be a second cholesterol-dependent mechanism whereby pneumococcal infection and the presence of pneumolysin increase hepatic sterol biosynthesis. Here we investigated a library of polymer particles varying in size and composition that allow for the cellular delivery of cholesterol and their effects on cell survival mechanisms following pneumolysin exposure. Intracellular delivery of cholesterol by nanocarriers composed of Eudragit E100–PLGA rescued pneumolysin-induced alterations of lipid homeostasis and enhanced cell survival irrespective of neutralization of pneumolysin.

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Section: Mechanism Of Protection From Pneumolysin By Nanoformulated Cholesterolsupporting

confidence: 88%

Intracellularly Released Cholesterol from Polymer-Based Delivery Systems Alters Cellular Responses to Pneumolysin and Promotes Cell Survival

et al. 2021

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“…Investigation of related materials with a newly created polymer, poly((n-butyl methacrylate)-co-(methyl methacrylate)-co-(2-(di methylamino) ethyl-methacrylate)) (PBMD), was demonstrated in a recent study (Figure 4, top). 67 Figure 4a shows cryo-TEM images of the formed polyplex materials suggesting globular aggregates. In situ SV analysis of populations by absorbance detection of the genetic material (pDNA, λ = 260 nm) identified several SV plateau values (Figure 4b).…”

Section: ■ Types Of Auc Experimentsmentioning

confidence: 99%

“…Figure 4d shows that all the essential components of the solution can be effectively separated by the AUC, making use of varying speed and time profiles. 67 Together with the nature of AUC being a quantitative technique, this opens the gate for an in-depth profiling of the complex nanomaterials and a better understanding of their behavior toward a potential application, not currently offered by using other analytical techniques.…”

Section: ■ Types Of Auc Experimentsmentioning

confidence: 99%

“…A typical net positive charge is known to enhance cellular uptake and, thereby, enhance a key step to tailor transfection efficiency. Investigation of related materials with a newly created polymer, poly(( n -butyl methacrylate)- co -(methyl methacrylate)- co -(2-(di methylamino) ethyl-methacrylate)) (PBMD), was demonstrated in a recent study (Figure , top) …”

Section: New Applications Of the Auc In The Life Sciencesmentioning

confidence: 99%

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Reincarnation of the Analytical Ultracentrifuge: Emerging Opportunities for Nanomedicine

Valderrama

Nischang

2021

Anal. Chem.

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The analytical ultracentrifuge (AUC) and the modern field of analytical ultracentrifugation found its inception approximately a century ago. We highlight the scope of its major experimental opportunities as a transport-based method, contemporary and up-and-coming investigation potential for polymers, polymer−drug conjugates, polymer assemblies, as well as medical nanoparticles. Special focus lies on molar mass estimates of unimeric polymeric species, selfassemblies in solution, and (co)localization of multicomponent systems in solution alongside the material−biofluid interactions. We close with present challenges and incentives for future research.

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“…9) Zudem scheinen hydrophobe Seitenketten den Gentransfer von Plasmid-DNA als auch von m/siRNA zu verbessern. 10) Die Abhängigkeit der endosomalen Freisetzung von Polyethylenimin (PEI) von der Pufferkapazität hat die Gruppe um Felius untersucht. Sie kombinierte PEI und pHsensitive Fluorophore in Kapseln derart, dass die pH-Sensoren im Inneren der Kapseln den lokalen pH-Wert detektierten (Abbildung 2).…”

Section: Wissenschaft + Forschungunclassified

Trendbericht: Makromolekulare Chemie

Gröschel¹,

Träger²,

Brendel³

2021

Nachr Chem

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Wie das letzte Jahr zeigte, ist die Forschung über Polymere und ihre Aggregate wichtig, um die Coronapandemie einzudämmen. Darüber hinaus gab es in der makromolekularen Forschung eine Vielzahl an Neuerungen und wegweisende Entwicklungen, etwa bei Materialien und polymerbasierter Elektronik. Dieser Trendbericht umfasst nicht nur Highlights, sondern präsentiert auch die Forschungsinteressen des akademischen Nachwuchses.

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Solely aqueous formulation of hydrophobic cationic polymers for efficient gene delivery

Cited by 26 publications

References 61 publications

Intracellularly Released Cholesterol from Polymer-Based Delivery Systems Alters Cellular Responses to Pneumolysin and Promotes Cell Survival

Intracellularly Released Cholesterol from Polymer-Based Delivery Systems Alters Cellular Responses to Pneumolysin and Promotes Cell Survival

Reincarnation of the Analytical Ultracentrifuge: Emerging Opportunities for Nanomedicine

Trendbericht: Makromolekulare Chemie

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