Solanum paniculatum L. decreases levels of inflammatory cytokines by reducing NFKB, TBET and GATA3 gene expression in vitro

Rios, Raimon; Silva, Hugo Bernardino Ferreira da; Carneiro, Norma Vilany Queiroz; Pires, Anaque de Oliveira; Carneiro, Tamires Cana Brasil; Costa, Ryan dos Santos; Marques, Caroline; Machado, Marta Santos Serafim; Velozo, Eudes da Silva; Silva, Telma M. G. da; Silva, Tania M. S. da; Conceição, Adilva de Souza; Alcântara-Neves, Neuza Maria; Figueiredo, Camila A.

doi:10.1016/j.jep.2017.07.014

Cited by 17 publications

(6 citation statements)

References 80 publications

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“…4 In addition, ethnopharmacological studies have revealed that different parts of S. paniculatum are used to treat anemia, tuberculosis, malaria, hypertension and disorders of the kidney; it is also an antifever agent. [5][6][7] Previous phytochemical investigations of S. paniculatum roots showed a predominance of steroids, saponins and alkaloids. 8 The major steroidal alkaloids reported were jurubidine, jurubine and solanine.…”

Section: Introductionmentioning

confidence: 99%

“…9,10 Pharmacological studies indicate that S. paniculatum root extract is able to inhibit gastric secretion in pylorus-ligated mice as well as to be effective against ulcer induced by stress in mice, both activities being related to the high concentration of 3-aminospirostane steroidal alkaloids. 8 Further biological activities such as antiviral, 11 anti-inflammatory 5 and anticarcinogenic effects 12 have been described elsewhere.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of 3‐aminospirostane alkaloids from roots of Solanum paniculatum L. with hepatoprotective activity

Gazolla

Marques

Silva

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Solanum paniculatum L., popularly known as jurubeba, has traditionally been used in Brazilian folk medicine for liver diseases. However, there is a lack of knowledge about the chemical characterization of 3‐aminospirostane alkaloids, an important class related to pharmacological activities. This work aimed to characterize the alkaloids using liquid chromatography with tandem mass spectrometry (LC/MS/MS) supported by molecular networking and theoretical calculations as well as to evaluate the contribution to hepatoprotective activity. Methods S. paniculatum roots were collected and macerated with MeOH/H2O (8:2) obtaining the crude extract (SP‐CE). From this, partition using EtOAc with pH variation yielded the alkaloidic fraction (SP‐AF). Both were evaluated in an acute liver injury model (100 and 200 mg/kg), after intraperitoneal administration of carbon tetrachloride (CCl4) in mice. AST (aspartate transaminase) and ALT (alanine transaminase) serum levels were investigated, as well as the histopathological characteristics. The SP‐CE and SP‐AF were analyzed by LC/MS/MS, using quadrupole/time‐of‐flight and ion‐trap systems. The alkaloids annotated by the GNPS molecular network had their structures defined using gas‐phase ionization and fragmentation reaction supported by theoretical calculations. Results The SP‐CE and SP‐AF decreased the ALT serum levels compared with the negative control. The group treated with the SP‐CE (at the highest dose) demonstrated a significant decrease of ALT. Hepatic cell degeneration decrease was observed mainly at the highest dose of the treatment. Detailed electrospray ionization MS/MS data allowed us to identify alkaloids not previously reported, to propose their gas‐phase reactions and to redefine the initial open ring fragmentation mechanism of the steroidal alkaloids with the jurubidine moiety. Conclusions The results allowed us to identify seven steroidal alkaloids from jurubeba and redefine the initial mechanism of fragmentation. A significant hepatoprotective effect was also demonstrated, corroborating its traditional use.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of 3‐aminospirostane alkaloids from roots of Solanum paniculatum L. with hepatoprotective activity

Gazolla

Marques

Silva

et al. 2020

Rapid Comm Mass Spectrometry

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“…Sterilized and filtered DEPC-treated water was used in all cDNA reactions. This step was performed as previously described by (Rios et al, 2017).…”

Section: Rna Extraction and Cdna Productionmentioning

confidence: 99%

Polymorphisms in the DAD1 and OXA1L genes are associated with asthma and atopy in a South American population

Pires

Queiroz

Silva

et al. 2018

Molecular Immunology

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“…The Solanaceae family is cultivated in Mexico since it has nutritional and medicinal properties conferred by its chemical composition (Gutiérrez et al, 2014;Benítez et al, 2018;Shajib et al, 2018). This group includes plants with antiinflammatory, antioxidant, anticancer and antithrombotic activities (Zhang et al, 2018;Silveyra et al, 2018;Rios et al, 2017;Zhuang et al, 2017). Although some medicinal properties are due to compounds derived from secondary metabolisms such as phenylpropanoids, flavonoids, alkaloids, acetogenins, polyketides, terpenes, steroids and carotenoids (Sultana and Asif., 2017;Paudel et al, 2017), others are due to compounds derived from primary metabolism such as bioactive proteins (Herbel et al, 2017;Moreno et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

CARACTERIZACIÒN PARCIAL DE UNA FRACCIÓN PROTEICA POTENCIALMENTE BIOACTIVA DE Solanum marginatum L. f.

et al. 2019

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Solanum marginatum L. f. (Solanaceae) is used in traditional Mexican medicine to treat infectious diseases and cancer, although lacks of the scientific validation. Consequently, we proposed a study of the enzymatic, antioxidant, antibacterial and toxic activities of an S. marginatum L. f. protein fraction previously characterized. For this, a protein extract from leaves of S. marginatum L. f. obtained and the protein fraction separated by FPLC. The protein content was quantified by the bicinchoninic acid (BCA) method and the enzymatic activity measured by the Kunitz method. Subsequently, protein subfractions were identified by SDS-PAGE. Additionally, the ability to capture radicals by the DPPH and ABTS methods was determined. The toxic effect was assessed with the A. salina test while cell viability in peripheral blood mononuclear cells (PBMC) tested by MTT method. Isolation of PF-FPLC was carried out at tr = 2.13 min, further, separated and identified seven subfractions with molecular weights ranging from 18-112 kDa, while the optimal conditions of enzymatic activity were 37°C, pH 7 and 120 min. The ABTS and DPPH methods showed a radical capture percentage of 20 and 62% respectively. The PF-FPLC proteins showed no toxicity in A. salina. However, the PF-FPLC showed a low antimicrobial effect (inhibition ≤ 35%), and decreases PBMC viability.

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Solanum paniculatum L. decreases levels of inflammatory cytokines by reducing NFKB, TBET and GATA3 gene expression in vitro

Cited by 17 publications

References 80 publications

Characterization of 3‐aminospirostane alkaloids from roots of Solanum paniculatum L. with hepatoprotective activity

Characterization of 3‐aminospirostane alkaloids from roots of Solanum paniculatum L. with hepatoprotective activity

Polymorphisms in the DAD1 and OXA1L genes are associated with asthma and atopy in a South American population

CARACTERIZACIÒN PARCIAL DE UNA FRACCIÓN PROTEICA POTENCIALMENTE BIOACTIVA DE Solanum marginatum L. f.

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