Soil lead pollution modifies the structure of arbuscular mycorrhizal fungal communities

Faggioli, Valeria Soledad; Menoyo, Eugenia; Geml, József; Kemppainen, Minna; Pardo, Alejandro Guillermo; Salazar, María Julieta; Becerra, Alejandra Gabriela

doi:10.1007/s00572-019-00895-1

Cited by 40 publications

(31 citation statements)

References 64 publications

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“…AM fungi can survive in various environments including Pb polluted areas, improve growth and stress tolerance of host plants (Faggioli et al 2019;Yang et al 2015;Zhang et al 2020b). Under Pb stress, AM fungi colonized plants were reported to have better growth (Chen et al 2005;Dhawi et al 2016;) and accumulate more Pb in root than in shoot (Yang et al 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Arbuscular mycorrhizal (AM) fungi can establish mutualistic symbioses with more than 80% of terrestrial plants in different ecosystems (Davison et al 2015;Parniske 2008) including Pb polluted areas (Faggioli et al 2019;Zhang et al 2020a). With AM fungal colonization, plants usually have higher biomass (Chen et al 2005;Huang et al 2017), increased antioxidant enzymes activities and photosynthetic rates, and showed improved Pb tolerance (Huang et al 2017;Zhang et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Arbuscular mycorrhizal fungi increase Pb uptake of colonized and non-colonized Medicago truncatula root and deliver extra Pb to colonized root

Zhang

Ren

Zheng

et al. 2021

Preprint

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Aims Arbuscular mycorrhizal (AM) fungi form symbiosis with terrestrial plants and improve lead (Pb) tolerance of host plants. The AM plants accumulate more Pb in root than their non-mycorrhizal counterparts. However, the direct contribution of the mycorrhizal pathway to host plant Pb uptake was less reported. Methods In this study, the AM fungi colonized and non-colonized root of Medicago truncatula was separated by a split-root system, and their differences in responding to Pb application was compared. Results Inoculation of Rhizophagus irregularis increased shoot biomass accumulation and transpiration, and decreased both colonized and non-colonized root biomass accumulation. Application of Pb in the non-colonized root compartment increased the colonization rate of R. irregularis and up-regulated the relative expressions of MtPT4 and MtBCP1 in the colonized root compartment. Inoculation of R. irregularis increased the Pb uptake in both colonized and non-colonized plant root, while R. irregularis transferred Pb to the colonized root. The Pb transferred through the mycorrhizal pathway had low mobility move from root to shoot, and might be sequestrated and compartmented by R. irregularis. Conclusions The Pb uptake of plant root might follow water flow that facilitated by the aquaporin MtPIP2. The quantification of Pb transfer via mycorrhizal pathway and the involvement of MtPIP2 deserve further study.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Arbuscular mycorrhizal fungi increase Pb uptake of colonized and non-colonized Medicago truncatula root and deliver extra Pb to colonized root

Zhang

Ren

Zheng

et al. 2021

Preprint

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“…However, AMF-specific 18S rRNA gene primers might be able to amplify more families and provide a broader view of the AMF community than fungal ITS2 primers [107]. In this regard, the primer pair AMV4.5NF/AMDGR [109] is widely used to characterize fungal members affiliated with the Glomeromycota using Illumina platforms [110][111][112]. These primers amplify a ~258 bp fragment internal to the 18S rRNA gene.…”

Section: Identification Of Fungi From Environmental Samplesmentioning

confidence: 99%

DNA Metabarcoding for the Characterization of Terrestrial Microbiota—Pitfalls and Solutions

et al. 2021

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Soil-borne microbes are major ecological players in terrestrial environments since they cycle organic matter, channel nutrients across trophic levels and influence plant growth and health. Therefore, the identification, taxonomic characterization and determination of the ecological role of members of soil microbial communities have become major topics of interest. The development and continuous improvement of high-throughput sequencing platforms have further stimulated the study of complex microbiota in soils and plants. The most frequently used approach to study microbiota composition, diversity and dynamics is polymerase chain reaction (PCR), amplifying specific taxonomically informative gene markers with the subsequent sequencing of the amplicons. This methodological approach is called DNA metabarcoding. Over the last decade, DNA metabarcoding has rapidly emerged as a powerful and cost-effective method for the description of microbiota in environmental samples. However, this approach involves several processing steps, each of which might introduce significant biases that can considerably compromise the reliability of the metabarcoding output. The aim of this review is to provide state-of-the-art background knowledge needed to make appropriate decisions at each step of a DNA metabarcoding workflow, highlighting crucial steps that, if considered, ensures an accurate and standardized characterization of microbiota in environmental studies.

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“…Spore identification, however, is a time- and energy-intensive task that is susceptible to variability in spore morphology as a function of regional variations, host species, and microbial age, making it challenging to differentiate between the spores of similar species [ 27 ]. In contrast, high-throughput sequencing [ 28 , 29 ] represents an increasingly robust and common approach to reliably studying AMF community structure and diversity. High-throughput approaches have been widely used in studies of AMF in the context of forestry [ 13 ], agriculture [ 30 , 31 ], and environmental remediation [ 32 ], while they have also been used to explore the relationship between slope position and soil depth on AMF community composition associated with specific plants.…”

Section: Introductionmentioning

confidence: 99%

High-throughput sequencing analysis of the rhizosphere arbuscular mycorrhizal fungi (AMF) community composition associated with Ferula sinkiangensis

Luo

Wang

et al. 2020

BMC Microbiol

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Background Ferula sinkiangensis is an increasingly endangered medicinal plant. Arbuscular mycorrhiza fungi (AMF) are symbiotic microorganisms that live in the soil wherein they enhance nutrient uptake, stress resistance, and pathogen defense in host plants. While such AMF have the potential to contribute to the cultivation of Ferula sinkiangensis, the composition of AMF communities associated with Ferula sinkiangensis and the relationship between these fungi and other pertinent abiotic factors still remains to be clarified. Results Herein, we collected rhizosphere and surrounding soil samples at a range of depths (0–20, 20–40, and 40–60 cm) and a range of slope positions (bottom, middle, top). These samples were then subjected to analyses of soil physicochemical properties and high-throughput sequencing (Illumina MiSeq). We determined that Glomus and Diversispora species were highly enriched in all samples. We further found that AMF diversity and richness varied significantly as a function of slope position, with this variation primarily being tied to differences in relative Glomus and Diversispora abundance. In contrast, no significant relationship was observed between soil depth and overall AMF composition, although some AMF species were found to be sensitive to soil depth. Many factors significantly affected AMF community composition, including organic matter content, total nitrogen, total potassium, ammonium nitrogen, nitrate nitrogen, available potassium, total dissolvable salt levels, pH, soil water content, and slope position. We further determined that Shannon diversity index values in these communities were positively correlated with total phosphorus, nitrate-nitrogen levels, and pH values (P < 0.05), whereas total phosphorus, total dissolvable salt levels, and pH were positively correlated with Chao1 values (P < 0.05). Conclusion In summary, our data revealed that Glomus and Diversispora are key AMF genera found within Ferula sinkiangensis rhizosphere soil. These fungi are closely associated with specific environmental and soil physicochemical properties, and these soil sample properties also differed significantly as a function of slope position (P < 0.05). Together, our results provide new insights regarding the relationship between AMF species and Ferula sinkiangensis, offering a theoretical basis for further studies of their development.

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Soil lead pollution modifies the structure of arbuscular mycorrhizal fungal communities

Cited by 40 publications

References 64 publications

Arbuscular mycorrhizal fungi increase Pb uptake of colonized and non-colonized Medicago truncatula root and deliver extra Pb to colonized root

Arbuscular mycorrhizal fungi increase Pb uptake of colonized and non-colonized Medicago truncatula root and deliver extra Pb to colonized root

DNA Metabarcoding for the Characterization of Terrestrial Microbiota—Pitfalls and Solutions

High-throughput sequencing analysis of the rhizosphere arbuscular mycorrhizal fungi (AMF) community composition associated with Ferula sinkiangensis

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