Sodium nitroprusside has leishmanicidal activity independent of iNOS

Kawakami, Natalia Yoshie; Tomiotto-Pellissier, Fernanda; Cataneo, Allan Henrique Depieri; Orsini, Tatiane Marcusso; Thomazelli, Ana Paula Fortes dos Santos; Conchon‐Costa, Ivete; Pavanelli, Wander Rogério

doi:10.1590/0037-8682-0266-2015

Cited by 8 publications

(5 citation statements)

References 39 publications

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“…Kawakami and Orsini et al demonstrated that the release of NO from sodium nitroprusside (Figure ), an iron-containing complex, enhanced the in vitro antileishmanial activity in macrophages infected with L. amazonensis.…”

Section: Leishmaniasismentioning

confidence: 96%

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Metal Compounds against Neglected Tropical Diseases

et al. 2018

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Many first-line treatments for neglected tropical diseases identified by the World Health Organization (WHO) are limited by one or more of the following: the development of drug resistance, toxicity, and side effects, lack of selectivity, narrow therapeutic indices, route of administration, and bioavailability. As such, there is an urgent need to develop viable alternatives to overcome these limitations. The following review provides an overview of all existing metal complexes studied and evaluates the status of these complexes on the respective disease of choice.

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Section: Leishmaniasismentioning

confidence: 96%

“…Kawakami and Orsini et al 203 demonstrated that the release of NO from sodium nitroprusside (Figure 42), an ironcontaining complex, enhanced the in vitro antileishmanial activity in macrophages infected with L. amazonensis. Sodium nitroprusside is a drug commonly used to lower blood pressure and to relieve heart failure.…”

Section: Chemical Reviewsmentioning

confidence: 99%

Metal Compounds against Neglected Tropical Diseases

et al. 2018

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“…[ 25 – 27 ]. In the current study, SNP was chosen as an exogenous NO-generating agent [ 28 ] to treat ML of T . spiralis , and the differential expressions of proteins were identified by a label-free analysis coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS).…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide

et al. 2018

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Trichinella spiralis mainly dwells in the muscle tissue of its host and is the main causative agent of trichinellosis in humans. Nitric oxide (NO), an important intracellular signaling molecule that may restrict pathogen growth in infected hosts, has been known for its anti-pathogenic activity, including resistance to T. spiralis. Herein, we applied label-free analysis to investigate the effect of sodium nitroprusside (SNP, a NO donor compound) on the proteome of T. spiralis muscle larvae (ML), followed by Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway cluster analyses. Of the 1,476 proteins detected in the ML, 121 proteins showed differential expression, including 50 significantly upregulated and 71 downregulated proteins. The functions of the 108 annotated proteins were primarily related to signal transduction, transcription/translation, material metabolism, protein synthesis/assembly/degradation, and stress/defense/antioxidation. Quantitative real-time polymerase chain reaction (qRT-PCR) assay verified that FRMD5 and CUT-1 gene expression levels were significantly increased, while COX2 gene expression level was significantly decreased. GO annotation and KEGG pathway analyses showed that the majority of differentially expressed proteins were mainly involved in the molecular function of the catalytic activity, biological process of the immune system process, metabolic process, cellular component organization, biological adhesion, and cellular component of the macromolecular complex. Our results demonstrate the first comprehensive protein expression profile of the ML in response to NO stress and provide novel references for understanding the potential mechanism underlying the effects of NO on trichinellosis.

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“…3-Nitrotyrosine is a well-established stable oxidative stress biomarker and a good predictor of the progression of some diseases, such as cardiovascular and neurodegenerative diseases [ 57 – 59 ]. The enhanced production of 3-nitrotyrosine was closely related to the enhanced microbicidal activity in Leishmania -infected macrophages [ 60 ]. A previous study revealed that 3-nitrotyrosine formation is a relevant indicator of antiparasitic activity [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolomics analysis of visceral leishmaniasis based on urine of golden hamsters

Yuan,

Chen,

Zhao

et al. 2023

Parasites Vectors

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Background Leishmaniasis is one of the most neglected tropical diseases and is spread mainly in impoverished regions of the world. Although many studies have focused on the host’s response to Leishmania invasion, relatively less is known about the complex processes at the metabolic level, especially the metabolic alterations in the infected hosts. Methods In this study, we conducted metabolomics analysis on the urine of golden hamsters in the presence or absence of visceral leishmaniasis (VL) using the ultra-performance liquid chromatography (UPLC) system tandem high-resolution mass spectrometer (HRMS). The metabolic characteristics of urine samples, along with the histopathological change and the parasite burden of liver and spleen tissues, were detected at 4 and 12 weeks post infection (WPI), respectively. Results Amino acid metabolism was extensively affected at both stages of VL progression. Meanwhile, there were also distinct metabolic features at different stages. At 4 WPI, the significantly affected metabolic pathways involved alanine, aspartate and glutamate metabolism, the pentose phosphate pathway (PPP), histidine metabolism, tryptophan metabolism and tyrosine metabolism. At 12 WPI, the markedly enriched metabolic pathways were almost concentrated on amino acid metabolism, including tyrosine metabolism, taurine and hypotaurine metabolism and tryptophan metabolism. The dysregulated metabolites and metabolic pathways at 12 WPI were obviously less than those at 4 WPI. In addition, seven metabolites that were dysregulated at both stages through partial least squares-discriminant analysis (PLS-DA) and receiver-operating characteristic (ROC) tests were screened to be of diagnostic potential. The combination of these metabolites as a potential biomarker panel showed satisfactory performance in distinguishing infection groups from control groups as well as among different stages of infection. Conclusion Our findings could provide valuable information for further understanding of the host response to Leishmania infection from the aspect of the urine metabolome. The proposed urine biomarker panel could help in the development of a novel approach for the diagnosis and prognosis of VL. Graphical Abstract

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Sodium nitroprusside has leishmanicidal activity independent of iNOS

Cited by 8 publications

References 39 publications

Metal Compounds against Neglected Tropical Diseases

Metal Compounds against Neglected Tropical Diseases

Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide

Metabolomics analysis of visceral leishmaniasis based on urine of golden hamsters

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