Sodium hypochlorite for sarcotesta remotion from papaya seeds: anatomical studies

Abstract

-Although papaya world production is high, the integument of papaya seeds, called sarcotesta, is an obstacle in the seedling production process. The aim of this work was to develop techniques to facilitate the removal of papaya seed sarcotesta for seedlings production. For that, two assays were developed. For the first one, the seeds were immersed in different concentrations of active chlorine (0, 2, 4, 6 and 8%) at ratio 600:200 seeds/mL of solution, respectively, besides the study of intact seeds and seeds subjected to the sieve method + stagnant water. For the second one, the seeds were immersed in active chlorine 2% in different ratios (10: 200; 200: 200; 400: 200 and 600: 200), and the concentration of 0% at ratio 10:200 and the sieve method + stagnant water. The effect of the treatments was evaluated by germination test and scanning electron microscopy. Sodium hypochlorite did not remove completely sarcotesta, but damaged it. In ratio 10:200 in a concentration of 2% of active chlorine for 24 hours, the embryo and the endosperm were not affected, resulting in 80% of germination, which can be an alternative to sarcotesta manual removal.

Métodos de assepsia em sementes de feijão

“…O hipoclorito de sódio tem sido comumente utilizado para desinfecção de sementes, visto sua eficácia na eliminação de patógenos e seu baixo efeito fitotóxico às sementes (ROCHA et al, 2014;CARMELLLO;CARDOSO, 2018). Corroboram com o presente trabalho a pesquisa realizada por Jesus et al (2015) que evidenciaram que o hipoclorito de sódio na concentração de 2% de cloro ativo não prejudica as partes essenciais para o processo de germinação de sementes de Carica papaya L, onde obtiveram 80% de sementes germinadas.…”

Limonium brasiliense (Boiss.) Kuntze Seeds: Disinfestation and Conservation of Viability Under Storage

“…Protocols involving the use of sodium hypochlorite solutions and ethanol 70% are common for seed sterilization, being successful in inhibiting the growth of bacteria and fungi (Barampuram et al, 2014;Fantinel et al, 2017). In some cases, sodium hypochlorite solutions can even increase germination by removing the seed coat without damaging the endosperm and embryo (Jesus et al, 2015).…”

Growth and Initial Development of Papaya Plants (Sunrise Solo) in Different Concentrations of Biostimulants

“…However, seed propagation is the most commercially used method in Brazil, since it is an accessible and practical procedure (Nguyen & Yen, 2018). However, it presents problems, such as slow and irregular germination, due to the presence of sarcotest in the seeds (Jesus et al, 2015), and dormancy associated with the maturation and harvesting time of the fruits (Melo et al, 2015), resulting in the increase of the cost of seeds and, consequently, high cost of production, and final value of the product.…”