Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2012.02.048 View full text
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Saurabh Gautam, Priyanka Dubey, Pranveer Singh, S. Kesavardhana, Raghavan Varadarajan, Munishwar N. Gupta

Abstract: Obtaining correctly folded proteins from inclusion bodies of recombinant proteins expressed in bacterial hosts requires solubilization with denaturants and a refolding step. Aggregation competes with the second step. Refolding of eight different proteins was carried out by precipitation with smart polymers. These proteins have different molecular weights, different number of disulfide bridges and some of these are known to be highly prone to aggregation. A high throughput refolding screen based upon fluorescen…

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