Small Scale Field Testing of PRSV Resistance in Transgenic Papaya Line Kn116/5

Phironrit, Namthip; Chowpongpang, Srimek; Warin, Nuchnard; Bhunchoth, Anjana; Attathom, S.

doi:10.17660/actahortic.2007.740.19

Cited by 6 publications

(3 citation statements)

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“…A small-scale field trial of the R 2 transgenic line (KN116/5) for its PRSV resistance and agronomic qualities was conducted from June 2003 to July 2004 in the field testing facility of the Plant Genetic Engineering Unit of BIOTEC, Kasetsart University located in Nakhon Pathom [82]. KN116/5, an advanced transgenic line derived from the Thai papaya variety Khak Nual, was found to be highly resistant (97%) to PRSV infection during the oneyear field test while the non-transgenic plants were all infected 2 months after planting.…”

Section: In Thailandmentioning

confidence: 99%

Recent advances in the development of transgenic papaya technology

Mendoza

Laurena

Botella

2008

Biotechnology Annual Review

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Abstract. Papaya with resistance to papaya ringspot virus (PRSV) is the first genetically modified tree and fruit crop and also the first transgenic crop developed by a public institution that has been commercialized. This chapter reviews the different transformation systems used for papaya and recent advances in the use of transgenic technology to introduce important quality and horticultural traits in papaya. These include the development of the following traits in papaya: resistance to PRSV, mites and Phytophthora, delayed ripening trait or long shelf life by inhibiting ethylene production or reducing loss of firmness, and tolerance or resistance to herbicide and aluminum toxicity. The use of papaya to produce vaccine against tuberculosis and cysticercosis, an infectious animal disease, has also been explored. Because of the economic importance of papaya, there are several collaborative and independent efforts to develop PRSV transgenic papaya technology in 14 countries. This chapter further reviews the strategies and constraints in the adoption of the technology and biosafety to the environment and food safety. Constraints to adoption include public perception, strict and expensive regulatory procedures and intellectual property issues.

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Section: In Thailandmentioning

confidence: 99%

Recent advances in the development of transgenic papaya technology

Mendoza

Laurena

Botella

2008

Biotechnology Annual Review

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“…In addition, the phenotypic characteristics need to be tested whether they are similar to the non-transgenic counterpart, which is important for regulatory purposes and variety release. In other transgenic crops agronomic traits and yield performance has been evaluated for instance, in transgenic squash that contained single or multiple virus coat protein gene (Tricoli et al 1995), burley tobacco that expresses tobacco vein mottling virus or alfalfa mosaic virus coat protein genes (Xu et al 1999), peanut resistant to tomato spotted wilt virus (Yang and Ozias-Akins 2004), tomato hybrids resistant to tomato spotted wilt virus (Accotto et al 2005) and papaya resistant to papaya ringspot virus Hawaiian isolate (Bau et al 2004;Ferreira et al 2002;Phironrit et al 2007;Tennant et al 2005). ere were either no or few di erences in agronomic traits of the transgenic crops in comparison to their non-transgenic counterparts.…”

mentioning

confidence: 99%

Evaluation of selected transgenic papaya (Carica papaya L.) lines for inheritance of resistance to papaya ringspot virus and horticultural traits

Retuta¹,

Magdalita²,

Aspuria³

et al. 2012

Plant Biotechnology

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Three selected transgenic papaya lines 124-3, 132-2 and 142-3 across the T 1 and T 2 generations were preliminarily evaluated under Biological Containment Level 2 (BL2) greenhouse approved by the National Committee on Biosafety of the Philippines (NCBP) to determine the partial stability of expression of resistance to papaya ringspot virus (PRSV) and selected phenotypic traits. e PRSV reaction of the three transgenic lines using mechanical inoculation test and enzyme-linked immunosorbent assay was assessed. e qualitative and quantitative horticultural traits at owering and ripe fruit stages of the transgenic lines were also evaluated. Based from preliminary results, the percentages of resistant progenies to PRSV in the T 1 generation were 83% for 124-3, 74% for 132-2, and 70% for 142-3. However, in the same generation, no di erence between the transgenic and non-transgenic were observed for stem, leaf and petiole color, fruit shape, peel, esh and seed color, stem diameter, number of days to rst ower, and fruit edible portion, indicating that the transgenic is seemingly stable. e percentage of resistant progenies to PRSV in the T 1 generation decreased in the T 2 generation, indicating that the inherited resistance of the three transgenic lines was unstable, and a continuous evaluation of the transgene could be done in later generations. In contrast, the light green (RHS 141B) stem color, dark green (RHS 141A) leaves and petioles and creamy white owers (RHS 155A) were stably expressed in both the T 1 and T 2 generations. e plant height for lines 124-3 and 142-3, internode length for line 142-3 and number of nodes to rst ower for lines 132-2 and 142-3 were also stable in both T 1 and T 2 generations. In addition, the pyriform fruit shape for hermaphrodite sex form and rounded fruit shape for the female sex form, light yellow orange (RHS 17A) peel color, bright yellow orange (RHS 23A) esh color and black (RHS 202A) seeds of the three transgenic lines were stably expressed in both generations. e fruit weight, total soluble solids and fruit edible portion for lines 124-3 and 142-3 was stably expressed in both generations.

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“…In Malaysia, the gene constructs from coat protein and Nib replicase genes of the local PRSV strain were made and transferred separately into tissues of 'Eksotika' papaya by Agrobacterium . Thailand successfully carried out a field trial of the transgenic PRSV resistant papayas and reported that a R 2 transgenic line KN116/5 was highly resistant with only 3% infection compared with 100% of non-transformed trees (Phironrit et al 2005). Yield was 40 times higher and fruit quality was good with TSS of 12…”

Section: Papaya Ringspot Virus Resistancementioning

confidence: 99%

Breeding Papaya (Carica papaya L.)

Chan

Breeding Plantation Tree Crops: Tropical Species

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Small Scale Field Testing of PRSV Resistance in Transgenic Papaya Line Kn116/5

Cited by 6 publications

References 0 publications

Recent advances in the development of transgenic papaya technology

Recent advances in the development of transgenic papaya technology

Evaluation of selected transgenic papaya (Carica papaya L.) lines for inheritance of resistance to papaya ringspot virus and horticultural traits

Breeding Papaya (Carica papaya L.)

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