Small molecule inducers of actinobacteria natural product biosynthesis

Alwali, Amir Y; Parkinson, Elizabeth I

doi:10.1093/jimb/kuad019

Cited by 5 publications

(2 citation statements)

References 108 publications

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“…Proteobacteria, comprising numerous pathogenic and commensal species, can modulate immune responses and are integral to maintaining the microbial balance in the gut ( 36 ). Actinobacteria, characterized by their role in organic matter decomposition, contribute to nutrient cycling and may have immunomodulatory effects on the host ( 37 ). The intricate interplay between these bacterial phyla in the gastrointestinal tract underscores their significance in promoting digestion, nutrient absorption, and overall animal well-being.…”

Section: Discussionmentioning

confidence: 99%

The distribution characteristics of aerosol bacteria in different types of sheepfolds

Wang,

Yu,

Raheem

et al. 2024

Front. Vet. Sci.

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With the development of modern sheep raising technology, the increasing density of animals in sheep house leads to the accumulation of microbial aerosols in sheep house. It is an important prerequisite to grasp the characteristics of bacteria in aerosols in sheep house to solve the problems of air pollution and disease prevention and control in sheep house. In this study, the microorganisms present in the air of sheep houses were investigated to gain insights into the structure of bacterial communities and the prevalence of pathogenic bacteria. Samples from six sheep pens in each of three sheep farms, totaling 18, were collected in August 2022 from Ningxia province, China. A high-volume air sampler was utilized for aerosol collection within the sheep housing followed by DNA extraction for 16S rRNA sequencing. Employing high-throughput 16S rRNA sequencing technology, we conducted an in-depth analysis of microbial populations in various sheep pen air samples, enabling us to assess the community composition and diversity. The results revealed a total of 11,207 operational taxonomic units (OTUs) within the bacterial population across the air samples, encompassing 152 phyla, 298 classes, 517 orders, 853 families, 910 genera, and 482 species. Alpha diversity and beta diversity analysis indicated that differences in species diversity, evenness and coverage between different samples. At the bacterial phylum level, the dominant bacterial groups are Firmicutes, Proteobacteria, and Actinobacteria, among which Firmicutes (97.90–98.43%) is the highest. At the bacterial genus level, bacillus, Bacteroides, Fusobacterium, etc. had higher abundance, with Bacillus (85.47–89.87%) being the highest. Through an in-depth analysis of microbial diversity and a meticulous examination of pathogenic bacteria with high abundance in diverse sheep house air samples, the study provided valuable insights into the microbial diversity, abundance, and distinctive features of prevalent pathogenic bacteria in sheep house air. These findings serve as a foundation for guiding effective disease prevention and control strategies within sheep farming environments.

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Section: Discussionmentioning

confidence: 99%

The distribution characteristics of aerosol bacteria in different types of sheepfolds

Wang,

Yu,

Raheem

et al. 2024

Front. Vet. Sci.

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“…Actinobacteria is known to produce a wide range of secondary metabolites and facilitate the development of AS through modulating the ubiquitination of IκB-α which may contribute to the activation of NF-κB signaling and the accumulation of proinflammatory factors in patients with AS [ 2 , 41 ]. A case-control study have reported that fecal microbiota in AS patients was characterized by a higher abundance of Actinobacteria [ 7 ].…”

Section: Discussionmentioning

confidence: 99%

Causal relationship between gut microbiota and ankylosing spondylitis and potential mediating role of inflammatory cytokines: A mendelian randomization study

Du,

Li,

Zhao

et al. 2024

PLoS ONE

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Associations between gut microbiota and ankylosing spondylitis have been discovered in previous studies, but whether these associations reflect a causal relationship remains inconclusive. Aiming to reveal the bidirectional causal associations between gut microbiota and ankylosing spondylitis, we utilized publicly available genome wide association study summary data for 211 gut microbiota (GM) taxa and ankylosing spondylitis (AS) to conduct two sample mendelian randomization analyses. Mediation analysis was performed to explore mediating inflammatory cytokines. We found that genetically predicted higher abundance of Lactobacillaceae family, Rikenellaceae family and Howardella genus had suggestive associations with decreased risk of ankylosing spondylitis while genetic proxied higher abundance of Actinobacteria class and Ruminococcaceae_NK4A214_group genus was associated with increased risk of ankylosing spondylitis. IL23 and IFN-γ were potential mediating cytokines for GM dysbiosis, especially for Actinobacteria class, leading to AS. Our study provided a new exploration direction for the treatment of AS. Lactobacillaceae family, Rikenellaceae family, Howardella genus, Actinobacteria class and Ruminococcaceae_NK4A214_group genus are expected to become new therapeutic targets and monitoring indicators for AS.

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Identification and characterization of the siderochelin biosynthetic gene cluster via coculture

Schaenzer,

Wang,

Hackenberger

et al. 2024

mBio

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Many microbial biosynthetic gene clusters (BGCs) are inactive under standard laboratory conditions, making characterization of their products difficult. Silent BGCs are likely activated by specific cues in their natural environment, such as the presence of competitors. Growth conditions such as coculture with other microbes, which more closely mimic natural environments, are practical strategies for inducing silent BGCs. Here, we utilize coculture to activate BGCs in nine actinobacteria strains. We observed increased production of the ferrous siderophores siderochelin A and B during coculture of Amycolatopsis strain WAC04611 and Tsukamurella strain WAC06889b. Furthermore, we identified the siderochelin BGC in WAC04611 and discovered that the GntR-family transcription factor sidR3 represses siderochelin production. Deletion of the predicted aminotransferase sidA abolished production of the carboxamides siderochelin A/B and led to the accumulation of the carboxylate siderochelin D. Finally, we deleted the predicted hydroxylase sidB and established that it is essential for siderochelin production. Our findings show that microbial coculture can successfully activate silent BGCs and lead to the discovery and characterization of unknown BGCs for molecules like siderochelin. IMPORTANCE Siderophores are vital iron-acquisition elements required by microbes for survival in a variety of environments. Furthermore, many siderophores are essential for the virulence of various human pathogens, making them a possible target for antibacterials. The significance of our work is in the identification and characterization of the previously unknown BGC for the siderophore siderochelin. Our work adds to the growing knowledge of siderophore biosynthesis, which may aid in the future development of siderophore-targeting pharmaceuticals and inform on the ecological roles of these compounds. Furthermore, our work demonstrates that combining microbial coculture with metabolomics is a valuable strategy for identifying upregulated compounds and their BGCs.

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Small molecule inducers of actinobacteria natural product biosynthesis

Cited by 5 publications

References 108 publications

The distribution characteristics of aerosol bacteria in different types of sheepfolds

The distribution characteristics of aerosol bacteria in different types of sheepfolds

Causal relationship between gut microbiota and ankylosing spondylitis and potential mediating role of inflammatory cytokines: A mendelian randomization study

Identification and characterization of the siderochelin biosynthetic gene cluster via coculture

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