Small Heat Shock Protein 20 Gene (Hsp20) of the Intertidal Copepod Tigriopus japonicus as a Possible Biomarker for Exposure to Endocrine Disruptors

Seo, Jung Soo; Park, T.-J.; Lee, Y.-M.; Park, Heum Gi; Yoon, Yong‐Dal; Lee, J.-S.

doi:10.1007/s00128-006-0957-3

Cited by 27 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The high expression levels of Bchsps contributed to activation of the HSR, and protected cells from oxidative stress. Previous studies have also shown that environmental stress (i.e., heavy metals, osmotic stress, and various chemicals) increased the expression of hsps (Rios-Arana et al 2005;Seo et al 2006;Ventura et al 2007;Wheelock et al 1999). The expression of hsps was significantly induced by typical temperature pressure and oxidative stress and reflected the characteristics of the hsps as "stress proteins.…”

Section: Discussionmentioning

confidence: 93%

Changes in the expression of four heat shock proteins during the aging process in Brachionus calyciflorus (rotifera)

Yang

Dong

et al. 2014

Cell Stress and Chaperones

View full text Add to dashboard Cite

Heat shock proteins (HSPs) are molecular chaperones and have an important role in the refolding and degradation of misfolded proteins, and these functions are related to aging. Rotifer is a useful model organism in aging research, owing to small body size (0.1-1 mm), short lifespan (6-14 days), and senescence phenotypes that can be measured relatively easily. Therefore, we used rotifer as a model to determine the role of four typical hsp genes on the aging process in order to provide a better understanding of rotifer aging. We cloned cDNA encoding hsp genes (hsp40, hsp60, hsp70, and hsp90) from the rotifer Brachionus calyciflorus Pallas, analyzed their molecular characteristics, determined its modulatory response under different temperatures and H 2 O 2 concentrations and investigated the changes in expression of these genes during the aging process. We found that Bchsp70 mRNA expression significantly decreased with aging. In addition, we also studied the effects of dietary restriction (DR) and vitamin E on rotifer lifespan and reproduction and analyzed the changes in expression of these four Bchsp genes in rotifers treated with DR and vitamin E. The results showed that DR extended the lifespan of rotifers and reduced their fecundity, whereas vitamin E had no significant effect on rotifer lifespan or reproduction. Real-time PCR indicated that DR increased the expression of these four Bchsps. However, vitamin E only improved the expression of Bchsp60, and reduced the expression of Bchsp40, Bchsp70, and Bchsp90. DR pretreatment also increased rotifer survival rate under paraquat-induced oxidative stress. These results indicated that hsp genes had an important role in the anti-aging process.

show abstract

Section: Discussionmentioning

confidence: 93%

Changes in the expression of four heat shock proteins during the aging process in Brachionus calyciflorus (rotifera)

Yang

Dong

et al. 2014

Cell Stress and Chaperones

View full text Add to dashboard Cite

show abstract

“…Consequently, the number of RNA-based studies in copepods has increased in recent years (e.g., Aruda et al, 2011;Barreto et al, 2011;Flowers and Burton, 2006;Hansen et al, 2008aHansen et al, , 2008bHansen et al, , 2009Hansen et al, , 2010Ki et al, 2009;Kim et al, 2011;Lauritano et al, 2011aLauritano et al, , 2011bLauritano et al, , 2012aRhee et al, 2009;Seo et al, 2006aSeo et al, , 2006bSeo et al, , 2006cTarrant et al, 2008;Voznesensky et al, 2004). Most of these studies focused on the gene expression of the stress response proteins in the model copepod species Calanus finmarchicus and Tigriopus japonicus.…”

Section: Introductionmentioning

confidence: 98%

An improved method for achieving high-quality RNA for copepod transcriptomic studies

Zhang

Finiguerra

Dam

et al. 2013

Journal of Experimental Marine Biology and Ecology

View full text Add to dashboard Cite

“…Due to the lack of information concerning the biological effects of PAEs and the advent of new technologies in functional genomic analysis, monitoring the expression of multiple genes involved in a wide spectrum of cellular processes and signaling pathways offers a potentially powerful method to study the mechanistic basis of toxic action and unravel the global effect underlying the observed adverse physiological effects of PAEs. A handful of studies have used gene expression analysis to assess the ecotoxicity of PAE exposure in aquatic wildlife invertebrates, such as Tigriopus japonicus and Chironomus riparius [26,27]. The high sensitivity of the N. denticulate hc gene to DPrP reported in a previous study has suggested that a gene expression profile may be potentially used as a biomarker tool for evaluating aquatic contamination by DPrP or other phthalate esters [22].…”

Section: Resultsmentioning

confidence: 99%

Differential Expression of Physiological Genes of Neocaridina Denticulate at the Transcriptional Level Following Short-Term Exposure to Non-Lethal Concentrations of Phthalate Esters

Ch¹,

Tsai²,

Sung³

2017

J Pollut Eff Cont

View full text Add to dashboard Cite

Xenobiotic phthalate esters (PAEs) are considered endocrine disrupting chemicals and are known to cause immunotoxicity, having been linked with the susceptibility of shrimp to pathogens; however, the effect on other physiological functions has not been explored. This study sought to understand the genetic responses of Neocaridina denticulate, a common freshwater shrimp, to three different PAEs, i.e., diethyl phthalate (DEP), dipropyl phthalate (DPrP), and diphenyl phthalate (DPP). The differential expression of 10 functional known expressed sequence tags derived from N. denticulate were analyzed by semi-quantitative RT-PCR after 24 h of exposure to non-lethal concentrations (0, 0.1, 1.0, and 10 mg/L) of PAEs in pond water. Compared with the control group, nine genes were differentially expressed in the DEP-treated groups, seven genes were differentially expressed in the DPrPtreated groups, and five genes were differentially expressed in the DPP-treated groups. These PAE-affected genes primarily belonged to three functional classes: defense-related genes (qm: QM protein hc: hemocyanin), metabolism-related genes (cathepsin-L like cysteine protease, catl; glutamate dehydrogenase, gdh; and inosine monophosphate dehydrogenase 1, impdh-1) and stress-related genes (hsp70: 70 kDa-heat shock protein; gst: glutathione S-transferase, and tps: trehalose-6-phosphate synthase). Among these, the stress-related genes were significantly up-regulated by DEP, DPrP, and DPP. These effects of PAEs on the expression of genes required for multiple physiological functions suggest that even with non-lethal concentrations of PAEs, a polluted aquatic environment may still present a potential risk to N. denticulate.

show abstract

Small Heat Shock Protein 20 Gene (Hsp20) of the Intertidal Copepod Tigriopus japonicus as a Possible Biomarker for Exposure to Endocrine Disruptors

Cited by 27 publications

References 0 publications

Changes in the expression of four heat shock proteins during the aging process in Brachionus calyciflorus (rotifera)

Changes in the expression of four heat shock proteins during the aging process in Brachionus calyciflorus (rotifera)

An improved method for achieving high-quality RNA for copepod transcriptomic studies

Differential Expression of Physiological Genes of Neocaridina Denticulate at the Transcriptional Level Following Short-Term Exposure to Non-Lethal Concentrations of Phthalate Esters

Contact Info

Product

Resources

About