Small hairpin RNA-mediated Krüppel-like factor 8 gene knockdown inhibits invasion of nasopharyngeal carcinoma

Abstract

Abstract. The present study aimed to characterize the expression of Krüppel-like factor 8 (KLF8) in nasopahryngeal carcinoma (NPC) cell lines and determine its effect on tumor development and invasion following KLF8 gene knockdown by small hairpin RNA (shRNA). KLF8 expression in four NPC cell lines was examined by quantitative polymerase chain reaction (qPCR) and western blotting. KLF8 was knocked down in the SUNE1-5-8F/Sh-KLF8 cell line using shRNA, and the resulting stable cell line SUNE1-5-8F-sh-KLF8 was transplanted into nude mice in order to observe tumor formation and invasion. The results obtained from qPCR and western blotting revealed that, of the four NPC cell lines, KLF8 expression was lowest in the CNE-1 cells and highest in the SUNE1-5-8F cells. The tumor xenograft mouse models revealed that SUNE1-5-8F/Sh-KLF8 cells had a reduced ability for tumor formation and invasion compared with the control group. These results demonstrated for the first time that KLF8 modulates the formation and invasive ability of nasopharyngeal carcinoma.

KLF8 is associated with poor prognosis and regulates glycolysis by targeting GLUT4 in gastric cancer

“…Understanding the prognostic factors or the risk for metastasis is intriguing and could affect clinical practice. 29 KLF8 is a GT-box (CACCC) binding dual-transcription factor 33 that is widely expressed in adult tissues. KLF8 is aberrantly expressed in several types of human tumours and its high expression is significantly correlated with oncogenic transformation and tumour progression.…”

Krüppel‐like factor 8 promotes cancer stem cell‐like traits in hepatocellular carcinoma through Wnt/β‐catenin signaling

“…Our results provide a novel concept for understanding the role of KLF8 in HCC distant metastasis that involves promotion of cancer stem cell-like traits through Wnt/b-catenin signaling. KLF8 has recently emerged as an important cancerpromoting protein in various types of human cancers [15,[24][25][26]. It is highly overexpressed in various invasive human cancers, and promotes cancer cell invasion and metastasis [15,25,27] by inducing cell cycle progression [28], transformation [29], and the epithelial to mesenchymal transition [30].…”

“…KLF8 has recently emerged as an important cancerpromoting protein in various types of human cancers [15,[24][25][26]. It is highly overexpressed in various invasive human cancers, and promotes cancer cell invasion and metastasis [15,25,27] by inducing cell cycle progression [28], transformation [29], and the epithelial to mesenchymal transition [30]. However, its exact role in HCC has not been determined.…”

Targeting the Warburg effect: A revisited perspective from molecular mechanisms to traditional and innovative therapeutic strategies in cancer

“…Krüppel-like transcription factor 8 (KLF8), as a GT box (CACCC) binding double transcription factor, is abnormally expressed in several types of human tumors. Its high expression is significantly related to carcinogenic transformation and tumor progression 97 . KLF8 activates GLUT4 promoter in a dose-dependent manner, and silencing KLF8 expression significantly reduces glucose uptake, lactate secretion and ATP production.…”