Small‐Diameter Silk Vascular Grafts (3 mm Diameter) with a Double‐Raschel Knitted Silk Tube Coated with Silk Fibroin Sponge

Aytemiz, Derya; Sakiyama, Wataru; Suzuki, Yuichi; Nakaizumi, Naoki; Tanaka, Ryou; Ogawa, Yoko; Takagi, Yoshihide; Nakazawa, Yasumoto; Asakura, Tetsuo

doi:10.1002/adhm.201200227

Cited by 73 publications

(80 citation statements)

References 24 publications

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“…19,33 Scanning electron microscopy (SEM; VE-7800 Keyence, Japan) was used to check the morphology of the SF grafts. 19 …”

Section: Vascular Graft Tubementioning

confidence: 99%

“…19,21,33 FIGURE 9. The results of analysis of histopathological examination of the 4 types of grafts.…”

Section: Coating Process Applied To Graft Tubesmentioning

confidence: 99%

“…18 Furthermore, formation of endothelial cells and smooth muscle cells were found in the earlystages. Although previous studies show that SF is useful for knitted tube material, 19,20 numerous problems still exist. The knitted SF tube cannot maintain its anastomotic strength.…”

Section: Introductionmentioning

confidence: 99%

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Effect of fibroin sponge coating on in vivo performance of knitted silk small diameter vascular grafts

et al. 2015

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ABSTRACT. Vascular grafts under 5 mm or less in diameter are not developed due to a problem caused by early thrombus formation, neointimal hyperplasia, etc. Bombyx mori silk fibroin (SF) which has biodegradability and tissue infiltration is focused as tube and coating material of vascular grafts. Coating is an important factor to maintain the strength of the anastomotic region of vascular grafts, and to prevent the blood leak from the vascular grafts after implantation. Therefore, in this research, we focused on the SF concentration of the coating solution, and tissue infiltration and remodeling were compared among each SF concentration. Silk poly (-ethylene) glycol diglycidyl ether (PGDE) coating with concentrations of 1.0%, 2.5%, 5.0%, and 7.5% SF were applied for the double-raschel knitted small-sized vessel with 1.5 mm diameter and 1cm in length. The grafts were implanted in the rat abdominal aorta and removed after 3 weeks or 3 months. Vascular grafts patency was monitored by ultrasound, and morphological evaluation was performed by histopathological examination. SF concentration had no significant effects on the patency rate. However, tissue infiltration was significantly higher in the sample of 2.5% SF in 3 weeks, and 1.0% and 2.5% SF in 3 months. Also, in comparison of length inside of the graft, stenosis were not found in 3 weeks, however, found with 5.0% and 7.5% in 3 months. From these results, it is clear that 2.5% SF coating is the most suitable concentration, based on the characteristics of less stenosis, early tissue infiltration, and less neointimal hyperplasia.

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“…19,33 Scanning electron microscopy (SEM; VE-7800 Keyence, Japan) was used to check the morphology of the SF grafts. 19 …”

Section: Vascular Graft Tubementioning

confidence: 99%

“…19,21,33 FIGURE 9. The results of analysis of histopathological examination of the 4 types of grafts.…”

Section: Coating Process Applied To Graft Tubesmentioning

confidence: 99%

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Effect of fibroin sponge coating on in vivo performance of knitted silk small diameter vascular grafts

et al. 2015

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“…When electrospun into small diameter vessels (1–3 mm diameter), silk fibroin grafts display good biocompatibility, remaining patent after 1 month and 85% patent after 12 months, outperforming PTFE control grafts in a rat abdominal aortic model [53]. Further improvements continue to be investigated to enhance endothelialization, which require 12 weeks to achieve 90% coverage.…”

Section: Natural Proteinsmentioning

confidence: 99%

Elastomers in vascular tissue engineering

Hiob

Crouch

Weiss

2016

Current Opinion in Biotechnology

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Elastomers are popular in vascular engineering applications, as they offer the ability to design implants that match the compliance of native tissue. By mimicking the natural tissue environment, elastic materials are able to integrate within the body to promote repair and avoid the adverse physiological responses seen in rigid alternatives that often disrupt tissue function. The design of elastomers has continued to evolve, moving from a focus on long term implants to temporary resorbable implants that support tissue regeneration. This has been achieved through designing chemistries and processing methodologies that control material behavior and bioactivity, while maintaining biocompatibility in vivo. Here we review the latest developments in synthetic and natural elastomers and their application in cardiovascular treatments.

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“…3 Their application to artificial blood vessels is also expected. 4 Thus, much attention has been focused on the mechanism of the structural transition from liquid silk to silk fibers not only to understand the structural origin of the expression of their excellent mechanical properties and biocompatibility but also to develop materials that are more advanced than silk fibers. [5][6][7][8][9][10][11] For the spectroscopic study of the mechanism, silk fibroin concentrated aqueous solutions that are formed by the lysis of silk fibers in LiBr or CaCl2/C2H5OH solutions at high temperatures, called regenerated silk fibroin solutions, have been used.…”

Section: Introductionmentioning

confidence: 99%

Structural Transition of Bombyx mori Liquid Silk Studied with Vibrational Circular Dichroism Spectroscopy

et al. 2015

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7As another intermediate structure, an α-helix-rich crystalline Silk I structure was also observed by X-ray scattering 2015 © The Japan Society for Analytical Chemistry † To whom correspondence should be addressed. Agriculture and Technology, Koganei, Japan We investigated the structural transition from liquid silk to silk fibers with vibrational circular dichroism spectroscopy. Liquid silk showed a major right-handed optically active band at around 1650 cm -1 and a minor one at around 1680 cm -1 . The former disappeared over time, while the intensity in the latter increased. With the former wavenumber, liquid silk mainly adopted a random-coil structure. In contrast, the latter may reflect an intermediate structure in the transition. Furthermore, two right-handed bands at around 1630 and 1660 cm -1 appeared with the disappearance of the major band, and then the wavenumber of the former shifted to around 1620 cm -1 . The shift results from the decrease in the frequency of the CO stretching mode due to the stacking of the β-sheet that comprises fibers. The band at 1660 cm -1 may reflect another intermediate structure due to its strong correlation with that at 1620 cm -1 in terms of their temporal change in intensity.

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Small‐Diameter Silk Vascular Grafts (3 mm Diameter) with a Double‐Raschel Knitted Silk Tube Coated with Silk Fibroin Sponge

Cited by 73 publications

References 24 publications

Effect of fibroin sponge coating on in vivo performance of knitted silk small diameter vascular grafts

Effect of fibroin sponge coating on in vivo performance of knitted silk small diameter vascular grafts

Elastomers in vascular tissue engineering

Structural Transition of Bombyx mori Liquid Silk Studied with Vibrational Circular Dichroism Spectroscopy

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