Small brown planthopper resistance loci in wild rice (Oryza officinalis)

Abstract

Host-plant resistance is the most practical and economical approach to control the rice planthoppers. However, up to date, few rice germplasm accessions that are resistant to the all three kinds of planthoppers (1) brown planthopper (BPH; Nilaparvata lugens Stål), (2) the small brown planthopper (SBPH; Laodelphax striatellus Fallen), and (3) the whitebacked planthopper (WBPH, Sogatella furcifera Horvath) have been identified; consequently, the genetic basis for host-plant broad spectrum resistance to rice planthoppers in a single variety has been seldom studied. Here, one wild species, Oryza officinalis (Acc. HY018, 2n = 24, CC), was detected showing resistance to the all three kinds of planthoppers. Because resistance to WBPH and BPH in O. officinalis has previously been reported, the study mainly focused on its SBPH resistance. The SBPH resistance gene(s) was (were) introduced into cultivated rice via asymmetric somatic hybridization. Three QTLs for SBPH resistance detected by the SSST method were mapped and confirmed on chromosomes 3, 7, and 12, respectively. The allelic/non-allelic relationship and relative map positions of the three kinds of planthopper resistance genes in O. officinalis show that the SBPH, WBPH, and BPH resistance genes in O. officinalis were governed by multiple genes, but not by any major gene. The data on the genetics of host-plant broad spectrum resistance to planthoppers in a single accession suggested that the most ideally practical and economical approach for rice breeders is to screen the sources of broad spectrum resistance to planthoppers, but not to employ broad spectrum resistance gene for the management of planthoppers. Pyramiding these genes in a variety can be an effective way for the management of planthoppers.

Omics-Based Comparative Transcriptional Profiling of Two Contrasting Rice Genotypes during Early Infestation by Small Brown Planthopper

“…02428). Pf9279-4 bears a major QTL Qsbph3d and two minor QTLs Qsbph7a and Qsbph12b [ 57 ]. The SBPH insects used for infestation were collected from rice fields in Jiangsu Province, China, that was confirmed to be non-viruliferous or viruliferous by dotimmunobinding assay and PCR detection [ 58 ].…”

“…When the seedlings were at the fourth leaf stage, weak plants were removed from the pots. In the insect-proof in greenhouse, second to fourth instar nymphs of the mixture of non-viruliferous and viruliferous SBPH population were introduced at a density of ten insects per seedling [ 57 ]. At each selected time-point, the two outmost layers of leaf sheaths were separated from the rice plants which had been fed and not fed by SBPH infestation, frozen immediately in liquid nitrogen and stored at −80 °C.…”

Comparative Proteomic Analysis of Susceptible and Resistant Rice Plants during Early Infestation by Small Brown Planthopper

“…In our previous studies, a wild species, Oryza officinalis (acc. HY018, 2n = 24, CC), was found to have high resistance to SBPH (Yan et al, 2004 ; Zhang et al, 2014 ). Three QTLs of resistance to SBPH have been identified in Pf9279-4, an introgression line derived from O. officinalis and O. sativa (cv.…”

“…Three QTLs of resistance to SBPH have been identified in Pf9279-4, an introgression line derived from O. officinalis and O. sativa (cv. 02428) by using asymmetric somatic hybridization, and are located on chromosome 3, 7, and 12 (Zhang et al, 2014 ). In this study, we analyzed the protein expression profile in rice leaf sheaths in response to SBPH infestation, aiming to explore the response of rice to SBPH infestation at protein level.…”

De Novo Transcriptome Sequencing of Oryza officinalis Wall ex Watt to Identify Disease-Resistance Genes

“…It is one of the three wild species indigenous in China [ 1 ]. It has reservoirs of many useful genes for rice breeding, such as resistance to blast, bacterial blight (BB), brown planthopper (BPH), and white backed planthopper (WBPH) [ 2 , 3 , 4 , 5 ]. It can also tolerate drought, cold, and other abiotic stresses [ 6 , 7 ].…”