SMAGP knockdown inhibits the malignant phenotypes of glioblastoma cells by inactivating the PI3K/Akt pathway

Ni, Hongzao; Ji, Daofei; Huang, Zhongdong; Li, Jing

doi:10.1016/j.abb.2020.108628

Cited by 7 publications

(4 citation statements)

References 31 publications

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“…Cell growth in vitro and xenograft tumor growth in vivo in BC tumors were inhibited where SH3BGRL2 was downregulated (46). SMAGP knock-down can inactivate the PI3K/Akt pathway, thereby inhibiting the malignant phenotypes of glioblastoma cells (47). Overall survival and recurrence-free survival were positively associated with NR4A1-NR4A3 expression.…”

Section: Discussionmentioning

confidence: 96%

Integrative Analysis of Homologous Recombination Repair Patterns Unveils Prognostic Signatures and Immunotherapeutic Insights in Breast Cancer

Li,

Jiang

2024

Preprint

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Background: Globally, breast cancer (BC) is the leading cause of female death and morbidity. Homologous recombination repair (HRR) is critical in BC. However, the prognostic role and immunotherapy response of HRR in BC remains to be clarified. Methods: Firstly, we identified HRR types in BC samples from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) dataset (GSE42568) based on 65 HRR genes (HRRGs). A differentially expressed genes (DEGs) list for different HRR types was generated. Then, the influences of gene sets composed of these DEGs on biological pathways and BC prognosis were explored. Next, we identified gene clusters based on gene sets composed of DEGs. Genes associated with prognosis for DEGs were identified using univariate Cox regression. Finally, the HRR score was constructed based on genes associated with prognosis. We analyzed how HRR score correlates with tumor mutation burden (TMB), immune cell infiltration (ICI), and immunotherapy response. Results: Three HRR clusters were discovered. HRR subtype A demonstrated decreased infiltration and a high number of immunosuppressive cells with a poor prognosis. DEGs among various HRR types were predominantly enriched in cell cycle and genomic stability-related pathways. The prognostic model based on sixteen DEGs accurately predicted BC prognosis. The HRRGs were differentially expressed in three DEGs clusters. TMB, ICI, and immunotherapy responses differed significantly between the high and low HRR groups (HSG, LSG). The HSG was distinguished by a high degree of ICI and low TMB. LSG had a better response to anti-PD-1 or anti-PD-1 and anti-CTLA4 combination therapy. Conclusion: This work revealed that HRR patterns would contribute to predicting prognosis and immunotherapy response in BC, which may benefit patients.

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Section: Discussionmentioning

confidence: 96%

Integrative Analysis of Homologous Recombination Repair Patterns Unveils Prognostic Signatures and Immunotherapeutic Insights in Breast Cancer

Li,

Jiang

2024

Preprint

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“…High DVL3 expression is associated with poor prognosis in patients with lung cancer ( 43 , 44 ). SMAGP encodes a transmembrane glycoprotein that plays an important role in tumor invasion and metastasis ( 45 – 47 ). STRN4 belongs to the striatin family and functions as a co-factor ( 48 ), particularly in cancer-related pathways ( 49 ) in prostate ( 50 ), lung ( 51 ), and gastric ( 52 ) cancers.…”

Section: Discussionmentioning

confidence: 99%

CD8+ T Cell-Associated Gene Signature Correlates With Prognosis Risk and Immunotherapy Response in Patients With Lung Adenocarcinoma

Zhang

Guo

et al. 2022

Front. Immunol.

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The presence of infiltrating CD8+ T lymphocytes in the tumor microenvironment of lung adenocarcinoma (LUAD) is correlated with improved patient prognosis, but underlying regulatory mechanisms remain unknown. To identify biomarkers to improve early diagnosis and treatment of LUAD, we downloaded 13 immune cell line-associated datasets from the GEO database. We identified CD8+ T cell-associated genes via weighted correlation network analysis. We constructed molecular subtypes based on CD8+ T cell-associated genes and constructed a multi-gene signature. We identified 252 CD8+ T cell-associated genes significantly enriched in immune function-related pathways and two molecular subtypes of LUAD (immune cluster 1 [IC1] and IC2) using our CD8+ T cell-associated gene signature. Patients with the IC2 subtype had a higher tumor mutation burden and lower immune infiltration scores, whereas those with the IC1 subtype were more sensitive to immune checkpoint inhibitors. Prioritizing the top candidate genes to construct a 10-gene signature, we validated our model using independent GSE and TCGA datasets to confirm its robustness and stable prognostic ability. Our risk model demonstrated good predictive efficacy using the Imvigor210 immunotherapy dataset. Thus, we established a novel and robust CD8+ T cell-associated gene signature, which could help assess prognostic risk and immunotherapy response in LUAD patients.

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“…Zhang et al, 2020). Studies have shown that the PI3K-AKT signaling pathway is a classic transduction pathway characteristic of most cancers and affects the prolifera-tion and apoptosis of tumor cells (H. Ni et al, 2020). In recent years, high-throughput sequencing technology has become the preferred method for analyzing tumor transcriptomes.…”

Section: Introductionmentioning

confidence: 99%

“…The PI3K–AKT signaling pathway, which plays an important role in maintaining homeostasis, is involved in various physiological processes and its over activation is a typical molecular feature of human malignant tumors (Y. Zhang et al., 2020). Studies have shown that the PI3K–AKT signaling pathway is a classic transduction pathway characteristic of most cancers and affects the proliferation and apoptosis of tumor cells (H. Ni et al., 2020). In recent years, high‐throughput sequencing technology has become the preferred method for analyzing tumor transcriptomes.…”

Section: Introductionmentioning

confidence: 99%

Selenium‐enriched peptides isolated from Cardamine violifolia are potent in suppressing proliferation and enhancing apoptosis of HepG2 cells

Yue

Cong

et al. 2022

Journal of Food Science

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Selenium (Se)-enriched peptides were isolated from Cardamine violifolia by enzymatic hydrolysis and ultrafiltration. S3 (molecular weight [MW] distribution of 3-5 kDa) exhibited the strongest inhibitory effect on HepG2 cells and was thus screened using the 3-(4,5-dimethylthiazole-2-yl) -2,5-diphenyltetrazolium bromide assay; it was found to have a high organic Se content. Its amino acid sequence was determined using HPLC-MS/MS. We then examined its ability to inhibit tumor cell proliferation and found that it arrested tumor cells in the S phase; moreover, it could induce cancer cell apoptosis. Following S3 treatment, we observed a decrease in mitochondrial membrane potential and an increase in cell calcium content. Upon S3 treatment at 60 µg/ml, the relative activities of caspase-3 and caspase-9 increased by 1.48 times and 2.17 times, and the contents of PI3K and AKT decreased from 2.05 ng/L and 1.95 ng/L to 0.71 ng/L and 0.50 ng/L, respectively, when compared with the control group. Transcriptomic analysis revealed significant changes in the PI3K-AKT pathway following S3 treatment. This study thus established a foundation for additional development of Se-enriched peptides from C. violifolia as a functional food.

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SMAGP knockdown inhibits the malignant phenotypes of glioblastoma cells by inactivating the PI3K/Akt pathway

Cited by 7 publications

References 31 publications

Integrative Analysis of Homologous Recombination Repair Patterns Unveils Prognostic Signatures and Immunotherapeutic Insights in Breast Cancer

Integrative Analysis of Homologous Recombination Repair Patterns Unveils Prognostic Signatures and Immunotherapeutic Insights in Breast Cancer

CD8+ T Cell-Associated Gene Signature Correlates With Prognosis Risk and Immunotherapy Response in Patients With Lung Adenocarcinoma

Selenium‐enriched peptides isolated from Cardamine violifolia are potent in suppressing proliferation and enhancing apoptosis of HepG2 cells

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