SLIRP, a Small SRA Binding Protein, Is a Nuclear Receptor Corepressor

Hatchell, Esme C.; Colley, Shane M.; Beveridge, Dianne J.; Epis, Michael R.; Stuart, Lisa M.; Giles, Keith M.; Redfern, Andrew; Miles, Lauren E.C.; Barker, Andrew; MacDonald, L.M.; Arthur, Peter G.; Lui, James K; Golding, Jemma L.; McCulloch, Ross K.; Metcalf, Cecily; Wilce, Jacqueline A.; Wilce, Matthew C. J.; Lanz, Rainer B.; O’Malley, Bert W.; Leedman, Peter J.

doi:10.1016/j.molcel.2006.05.024

Cited by 117 publications

(156 citation statements)

References 35 publications

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“…By its interaction with SLIRP (SRA stem-loop interacting RNA binding protein), SRA1 has a repressive function by recruiting corepressors (reviewed in (Liu et al 2016)). SLIRP represses NR transactivation in an SRA1-dependent manner (Hatchell, et al 2006). Interestingly, Slirp knockout mice are sub-fertile and males have defects in sperm motility and mitochondrial morphology .…”

Section: Mitochondrial-encoded Lncrnas and Lncrna Imported Into Mitocmentioning

confidence: 99%

Non-coding RNAs: long non-coding RNAs and microRNAs in endocrine-related cancers

Klinge

2018

Endocrine-Related Cancer

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Abstract:The human genome is 'pervasively transcribed' leading to a complex array of noncoding RNAs (ncRNAs) that far outnumber coding mRNAs. ncRNAs have regulatory roles in transcription and post-transcriptional processes as well numerous cellular functions that remain to be fully described. Best characterized of the 'expanding universe' of ncRNAs are the ~ 22 nucleotide microRNAs (miRNAs) that base-pair to target mRNA's 3' untranslated region within the RNA-induced silencing complex (RISC) and block translation and may stimulate mRNA transcript degradation. Long non-coding RNAs (lncRNAs) are classified as >200 nucleotides in length, but range up to several kb and are heterogeneous in genomic origin and function.LncRNAs fold into structures that interact with DNA, RNA, and proteins to regulate chromatin dynamics, protein complex assembly, transcription, telomere biology, and splicing. Some lncRNAs act as sponges for miRNAs and decoys for proteins. Nuclear-encoded lncRNAs can be taken up by mitochondria and lncRNAs are transcribed from mtDNA. Both miRNAs and lncRNAs are dysregulated in endocrine cancers. This review provides an overview on the current understanding of the regulation and function of selected lncRNAs and miRNAs, and their interaction, in endocrine-related cancers: breast, prostate, endometrial, and thyroid.

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Section: Mitochondrial-encoded Lncrnas and Lncrna Imported Into Mitocmentioning

confidence: 99%

Non-coding RNAs: long non-coding RNAs and microRNAs in endocrine-related cancers

Klinge

2018

Endocrine-Related Cancer

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“…A recent report shows a role for a ~700-nt lncRNA, SRA (steroid receptor RNA activator), in CTCF-mediated insulation. 51 SRA, which is reported to act as both a transcriptional co-activator and co-repressor, [52][53][54] is a chromatin-associated lncRNA found in a complex with the DEAD-box RNA helicase p68 (DDX5) and CTCF (Fig. 1B).…”

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confidence: 99%

Transcription control by long non-coding RNAs

2012

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“…1A) and SRA-STR7 (9). SRA-STR7 is bound by two NR corepressors: SRA stem loop interacting RNA binding protein and silencing mediator for retinoid or thyroidhormone receptors (SMRT)/histone deacetylase (HDAC)-associated repressor protein (18). Using radioloabeled SRA-STR1 in RNA electrophoretic mobility shift assays (REMSAs) and UV cross-link (UVXL) assays with protein extracts from MCF-7 and MDA-MB-468 breast, and HeLa cancer cell lines, we identified specific nuclear SRA-STR1 protein complexes in each cell line ( Fig.…”

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RNA-induced silencing complex (RISC) Proteins PACT, TRBP, and Dicer are SRA binding nuclear receptor coregulators

Redfern

Colley

Beveridge

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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The cytoplasmic RNA-induced silencing complex (RISC) contains dsRNA binding proteins, including protein kinase RNA activator (PACT), transactivation response RNA binding protein (TRBP), and Dicer, that process pre-microRNAs into mature microRNAs (miRNAs) that target specific mRNA species for regulation. There is increasing evidence for important functional interactions between the miRNA and nuclear receptor (NR) signaling networks, with recent data showing that estrogen, acting through the estrogen receptor, can modulate initial aspects of nuclear miRNA processing. Here, we show that the cytoplasmic RISC proteins PACT, TRBP, and Dicer are steroid receptor RNA activator (SRA) binding NR coregulators that target steroid-responsive promoters and regulate NR activity and downstream gene expression. Furthermore, each of the RISC proteins, together with Argonaute 2, associates with SRA and specific pre-microRNAs in both the nucleus and cytoplasm, providing evidence for links between NR-mediated transcription and some of the factors involved in miRNA processing.noncoding RNA | androgen action | prostate cancer M icroRNAs (miRNAs) are 22-nt noncoding RNAs that regulate gene expression by associating with the multiprotein RNA-induced silencing complex (RISC) and guiding RISC to silence specific mRNA species by mRNA degradation or translational inhibition (1). miRNAs are derived from large primary RNA transcripts, which are processed in the nucleus by Drosha/ Pasha into ∼70-nt precursor (pre-miRNA) duplexes. These duplexes are exported to the cytoplasm, and their conversion to mature 22-nt miRNAs is mediated by a transactivation response (TAR) RNA binding protein (TRBP)-Dicer complex (1), which provides a platform for RISC assembly and aids recruitment of Argonaute 2 (Ago2), the catalytic enzyme of RISC required for miRNA processing (2, 3). Protein kinase RNA (PKR) activator (PACT) is also integral to the RISC complex and associates with Dicer (4). TRBP and PACT are critical for efficient miRNA processing, because their depletion results in decreased mature miRNA levels and target gene silencing (2, 4, 5).Ligand-activated signaling by nuclear receptors (NRs) plays a key role in the promotion of human tumorigenesis. NR coregulators are a group of >300 molecules that act to fine tune ligand-induced gene transcription by acting as either coactivators [e.g., steroid receptor coactivator-1 (SRC-1)] or corepressors [e.g., nuclear receptor corepressor1 (NCoR1)] (6). One of the NR coregulators, steroid receptor RNA activator (SRA), is unique, because its RNA transcript functions to coactivate NR activity (7-9).Several links between miRNA pathways and NR action have been reported. miRNAs can regulate NR signaling at different levels, including targeting NRs [e.g., estrogen receptor-α (ERα)] (10, 11) or NR coregulator transcripts (e.g., amplified in breast cancer 1) (12). Conversely, NRs regulate an array of miRNAs in endocrine tissues (13). For example, ERα can regulate miRNA-21 expression at the transcriptional level (14) and the...

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SLIRP, a Small SRA Binding Protein, Is a Nuclear Receptor Corepressor

Cited by 117 publications

References 35 publications

Non-coding RNAs: long non-coding RNAs and microRNAs in endocrine-related cancers

Non-coding RNAs: long non-coding RNAs and microRNAs in endocrine-related cancers

Transcription control by long non-coding RNAs

RNA-induced silencing complex (RISC) Proteins PACT, TRBP, and Dicer are SRA binding nuclear receptor coregulators

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