Sleep homeostasis in<i>Drosophila</i>: a window on the vital function of sleep

Holland, Christian

doi:10.1093/biohorizons/hzy009

Cited by 2 publications

(6 citation statements)

References 61 publications

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“…( B, C ) During ad lib feeding, sleep in R23E10>Dop1R1 RNAi and R55B01 > Dop1R1 RNAi flies does not differ from R23E10/+ , Dop1R1 RNAi /+, or R55B01/+ parental controls ( n = 13–16 flies/group ANOVA for Genotype F[ 2 , 42 ] = 2.8, p = 0.07 and ANOVA for Genotype F[ 2 , 41 ] = 1.9, p = 0.15 for R23E10 and R55B01, respectively). ( D ) Daytime sleep was not altered in either R23E10>Dop1R1 RNAi or R55B01 > Dop1R1 RNAi flies compared to both parental controls (ANOVA for Genotype F[ 2 , 42 ] = 2.8, p = 0.02 and F[ 2 , 41 ] = 2.9, p = 0.06, * p < 0.05, modified Bonferroni test). ( E ) Nighttime sleep was not changed in either R23E10>Dop1R1 RNAi or R55B01 > Dop1R1 RNAi flies compared to parental controls (ANOVA for Genotype F[ 2 , 42 ] = 0.9, p = 0.40 and ANOVA for Genotype F[ 2 , 41 ] = 0.79, p = 0.46, * p < 0.05, modified Bonferroni test).…”

Section: Resultsmentioning

confidence: 99%

“… ( A ) Protocol for time-restricted feeding; flies were given food during ZT 8 AM to 5 PM. ( B, C ) During ad lib feeding, sleep in R23E10>Dop1R1 RNAi and R55B01 > Dop1R1 RNAi flies does not differ from R23E10/+ , Dop1R1 RNAi /+, or R55B01/+ parental controls ( n = 13–16 flies/group ANOVA for Genotype F[ 2 , 42 ] = 2.8, p = 0.07 and ANOVA for Genotype F[ 2 , 41 ] = 1.9, p = 0.15 for R23E10 and R55B01, respectively). ( D ) Daytime sleep was not altered in either R23E10>Dop1R1 RNAi or R55B01 > Dop1R1 RNAi flies compared to both parental controls (ANOVA for Genotype F[ 2 , 42 ] = 2.8, p = 0.02 and F[ 2 , 41 ] = 2.9, p = 0.06, * p < 0.05, modified Bonferroni test).…”

Section: Resultsmentioning

confidence: 99%

“…The intrinsic properties of R23E10 neurons have been evaluated in the context of sleep loss where it seems they monitor redox processes as an indicator of energy metabolism [ 19 , 38 , 39 ]. Thus, R23E10 neurons may serve as command neurons of sorts that can integrate stimuli from competing internal states to gate information flow [ 40 , 41 ]. Both the input and the output connections to R23E10 are well documented [ 25 , 26 , 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

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Sleep-promoting neurons remodel their response properties to calibrate sleep drive with environmental demands

et al. 2022

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Falling asleep at the wrong time can place an individual at risk of immediate physical harm. However, not sleeping degrades cognition and adaptive behavior. To understand how animals match sleep need with environmental demands, we used live-brain imaging to examine the physiological response properties of the dorsal fan-shaped body (dFB) following interventions that modify sleep (sleep deprivation, starvation, time-restricted feeding, memory consolidation) in Drosophila. We report that dFB neurons change their physiological response-properties to dopamine (DA) and allatostatin-A (AstA) in response to different types of waking. That is, dFB neurons are not simply passive components of a hard-wired circuit. Rather, the dFB neurons intrinsically regulate their response to the activity from upstream circuits. Finally, we show that the dFB appears to contain a memory trace of prior exposure to metabolic challenges induced by starvation or time-restricted feeding. Together, these data highlight that the sleep homeostat is plastic and suggests an underlying mechanism.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sleep-promoting neurons remodel their response properties to calibrate sleep drive with environmental demands

et al. 2022

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“…and R55B01>Dop1R1 RNAi flies does not differ from R23E10/+, Dop1R1 RNAi /+ or R55B01/+ parental controls (n=13-16 flies/group ANOVA for Genotype F[2,42]=2.8, p=0.07 and ANOVA for Genotype F[2,41]=1.9, p=0 15. for R23E10 and R55B01 respectively.…”

mentioning

confidence: 88%

“…The intrinsic properties of R23E10 neurons have been evaluated in the context of sleep loss where it seems they monitor redox processes as an indicator of energy metabolism [19, 38, 39]. Thus, R23E10 neurons may serve as command neurons of sorts that can integrate stimuli from competing internal states to gate information flow [40, 41]. Both the input and output connections to R23E10 are well documented [25, 26, 35, 36].…”

Section: Introductionmentioning

confidence: 99%

Sleep promoting neurons remodel their response properties to calibrate sleep drive with environmental demands.

Dissel

Klose

Swinderen

et al. 2021

Preprint

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Falling asleep at the wrong time can place an individual at risk of immediate physical harm. However, not sleeping degrades cognition and adaptive behavior. To understand how animals match sleep need with environmental demands, we used live-brain imaging to examine the physiological response properties of the Drosophila sleep homeostat (dFB) following interventions that modify sleep (sleep deprivation, starvation, time-restricted feeding, memory consolidation). We report that dFB neurons can distinguish between different types of waking and can change their physiological response-properties accordingly. That is, dFB neurons are not simply passive components of a hard-wired circuit. Rather, the dFB neurons themselves can determine their response to the activity from upstream circuits. Finally, we show that the dFB appears to contain a memory trace of prior exposure to metabolic challenges induced by starvation or time-restricted feeding. Together these data highlight that the sleep homeostat is plastic and suggests an underlying mechanism.

show abstract

Sleep homeostasis inDrosophila: a window on the vital function of sleep

Cited by 2 publications

References 61 publications

Sleep-promoting neurons remodel their response properties to calibrate sleep drive with environmental demands

Sleep-promoting neurons remodel their response properties to calibrate sleep drive with environmental demands

Sleep promoting neurons remodel their response properties to calibrate sleep drive with environmental demands.

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