2016
DOI: 10.1080/21624054.2016.1230585
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Abstract: In C. elegans research, transcriptional activation of glutathione S-transferase 4 (gst-4) is often used as a read-out for SKN-1 activity. While many heed an assumed non-exclusivity of the GFP reporter signal driven by the gst-4 promoter to SKN-1, this is also often ignored. We here show that gst-4 can also be transcriptionally activated by EOR-1, a transcription factor mediating effects of the epidermal growth factor (EGF) pathway. Along with enhancing exogenous oxidative stress tolerance, EOR-1 independently … Show more

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Cited by 26 publications
(25 citation statements)
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References 51 publications
(95 reference statements)
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“…The induction of the gcs-1p::GFP in positive control worms was used to indicate Phase II detox skn-1 activation. The gcs-1 reporter was chosen because, unlike gst-4 , it can only be activated by skn-1 [ 42 ]. A one hour heat shock at 37 °C with a four hour recovery at 20 °C was used as a positive control, modified from An et al., 2003 [ 35 ].…”
Section: Methodsmentioning
confidence: 99%
“…The induction of the gcs-1p::GFP in positive control worms was used to indicate Phase II detox skn-1 activation. The gcs-1 reporter was chosen because, unlike gst-4 , it can only be activated by skn-1 [ 42 ]. A one hour heat shock at 37 °C with a four hour recovery at 20 °C was used as a positive control, modified from An et al., 2003 [ 35 ].…”
Section: Methodsmentioning
confidence: 99%
“…Royalactin (also known as Major Royal Jelly Protein 1 or MRJP1) was prepared from RJ via ultracentrifugation, according to 6,7 . Royalactin-containing NGM plates were prepared by adding 1.7 µg mL -1 royalactin (unless stated otherwise) to liquid NGM immediately before pouring the plates.…”
Section: Royalactin Preparationmentioning
confidence: 99%
“…In essence, GFP is irreversibly targeted for proteasomal proteolysis. Fluorescence was quantified analogous to the automated method described in Detienne et al 7 , using a microplate reader to measure thousands of nematodes simultaneously (Flexstation II, Molecular Devices).…”
Section: In Vivo 20s Proteasome Activitymentioning
confidence: 99%
See 1 more Smart Citation
“…Our data reveal that glucose-fed bacteria which are high in AGEs promote an environment that causes animals to exhibit signs of oxidative stress including strong and consistent suppression of gst-4 (glutathione transferase-4) involved in the regulation of the Phase II oxidative stress response 33 . Analysis of transcriptional activation of gst-4 is often used as a read-out for oxidative stress tolerance 34 . Therefore, we next generated a loss of function allele of gst-4 by CRISPR/Cas9.…”
mentioning
confidence: 99%