Skin‐specific expression of <i>ictacalcin</i>, a homolog of the <i>S100</i> genes, during zebrafish embryogenesis

Hsiao, Chung-Der; Ekker, Marc; Tsai, Huai‐Jen

doi:10.1002/dvdy.10411

Cited by 18 publications

(9 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Zebrafish S100I.1 (Ictacalcin) expression (Figure 11) was previously described by [18] and [36]. Our data are consistent with their findings of expression in the epithelial cells of barbels, gills, olfactory rosettes and skin.…”

Section: Resultssupporting

confidence: 93%

Structural and functional diversification in the teleost S100 family of calcium-binding proteins

2008

View full text Add to dashboard Cite

BackgroundAmong the EF-Hand calcium-binding proteins the subgroup of S100 proteins constitute a large family with numerous and diverse functions in calcium-mediated signaling. The evolutionary origin of this family is still uncertain and most studies have examined mammalian family members.ResultsWe have performed an extensive search in several teleost genomes to establish the s100 gene family in fish. We report that the teleost S100 repertoire comprises fourteen different subfamilies which show remarkable similarity across six divergent teleost species. Individual species feature distinctive subsets of thirteen to fourteen genes that result from local gene duplications and gene losses. Eight of the fourteen S100 subfamilies are unique for teleosts, while six are shared with mammalian species and three of those even with cartilaginous fish. Several S100 family members are found in jawless fish already, but none of them are clear orthologs of cartilaginous or bony fish s100 genes. All teleost s100 genes show the expected structural features and are subject to strong negative selection. Many aspects of the genomic arrangement and location of mammalian s100 genes are retained in the teleost s100 gene family, including a completely conserved intron/exon border between the two EF hands. Zebrafish s100 genes exhibit highly specific and characteristic expression patterns, showing both redundancy and divergence in their cellular expression. In larval tissue expression is often restricted to specific cell types like keratinocytes, hair cells, ionocytes and olfactory receptor neurons as demonstrated by in situ hybridization.ConclusionThe origin of the S100 family predates at least the segregation of jawed from jawless fish and some extant family members predate the divergence of bony from cartilaginous fish. Despite a complex pattern of gene gains and losses the total repertoire size is remarkably constant between species. On the expression level the teleost S100 proteins can serve as precise markers for several different cell types. At least some of their functions may be related to those of their counterparts in mammals. Accordingly, our findings provide an excellent basis for future studies of the functions and interaction partners of s100 genes and finally their role in diseases, using the zebrafish as a model organism.

show abstract

Section: Resultssupporting

confidence: 93%

Structural and functional diversification in the teleost S100 family of calcium-binding proteins

2008

View full text Add to dashboard Cite

show abstract

“…2F). As previously reported with ictacalcin (Hsiao et al, 2003), by 72 hpf and until the larval-juvenile transition, the epithelial cells covering the entire larva were all rbp4-positive except for the neuromasts deposited in the cephalic and trunk lateral lines (Figs. 2C,G, 3A,C).…”

Section: Spatiotemporal Expressionsupporting

confidence: 81%

Epidermal transient down‐regulation of retinol‐binding protein 4 and mirror expression of apolipoprotein Eb and estrogen receptor 2a during zebrafish fin and scale development

Tingaud‐Sequeira¹,

Forgue²,

André³

et al. 2006

Developmental Dynamics

View full text Add to dashboard Cite

Very little is known about the molecular control of skin patterning and scale morphogenesis in teleost fish. We have found radially symmetrical epidermal placodes with down-regulation of retinol-binding protein 4 (rbp4) expression during the initial paired fin and scale morphogenesis in zebrafish (Danio rerio). This finding may be related to changes in keratinocyte cytodifferentiation and/or the integument retinoid metabolism. rbp4 transcripts are expressed afterward in the central epidermis of the scale papilla and gradually extend to the epidermis, covering the growing scale, whereas no transcripts were detected in posterior margin epidermis. In contrast, induction of apolipoprotein Eb (apoeb) and up-regulation of estrogen receptor 2a (esr2a) transcripts were observed in the epidermis at initiator sites of zebrafish ectodermal/dermal appendage morphogenesis. This expression was maintained in the posterior margin epidermis of the formed scales. esr2a was also strongly expressed in neuromasts, whereas no rbp4 and apoeb transcripts were detected in these mechanosensory structures. The observed epidermal molecular events suggest that epidermis patterning is due to an activator-inhibitor mechanism operational at epidermal-dermal interaction sites. rbp4 transcript expression was also strongly down-regulated by 1-phenyl-2-thio-urea (PTU). As this inhibitor is commonly used to block obscuring pigmentation during in situ hybridization studies, this finding suggests that PTU should be used with caution, particularly in studying skin development. Developmental Dynamics 235:3071-3079, 2006.

show abstract

“…The top ten most abundant unigenes are displayed in Table 2 and the most abundant unigene was annotated as ictacalcin according to the result of blast search against the NCBI nr protein database (e-value = 1.83E-29). This gene encodes a calcium binding protein and was reported to be highly expressed in the skin of zebrafish embryos [46]. The rest of most abundant unigenes were annotated as keratin, ribosomal proteins, cytochrome c oxidase subunit, senescence-associated protein and ATP synthase subunit beta, respectively (Table 2).…”

Section: Resultsmentioning

confidence: 99%

De Novo Assembly of Mud Loach (Misgurnus anguillicaudatus) Skin Transcriptome to Identify Putative Genes Involved in Immunity and Epidermal Mucus Secretion

Long

Zhou

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

Fish skin serves as the first line of defense against a wide variety of chemical, physical and biological stressors. Secretion of mucus is among the most prominent characteristics of fish skin and numerous innate immune factors have been identified in the epidermal mucus. However, molecular mechanisms underlying the mucus secretion and immune activities of fish skin remain largely unclear due to the lack of genomic and transcriptomic data for most economically important fish species. In this study, we characterized the skin transcriptome of mud loach using Illumia paired-end sequencing. A total of 40364 unigenes were assembled from 86.6 million (3.07 gigabases) filtered reads. The mean length, N50 size and maximum length of assembled transcripts were 387, 611 and 8670 bp, respectively. A total of 17336 (43.76%) unigenes were annotated by blast searches against the NCBI non-redundant protein database. Gene ontology mapping assigned a total of 108513 GO terms to 15369 (38.08%) unigenes. KEGG orthology mapping annotated 9337 (23.23%) unigenes. Among the identified KO categories, immune system is the largest category that contains various components of multiple immune pathways such as chemokine signaling, leukocyte transendothelial migration and T cell receptor signaling, suggesting the complexity of immune mechanisms in fish skin. As for mucin biosynthesis, 37 unigenes were mapped to 7 enzymes of the mucin type O-glycan biosynthesis pathway and 8 members of the polypeptide N-acetylgalactosaminyltransferase family were identified. Additionally, 38 unigenes were mapped to 23 factors of the SNARE interactions in vesicular transport pathway, indicating that the activity of this pathway is required for the processes of epidermal mucus storage and release. Moreover, 1754 simple sequence repeats (SSRs) were detected in 1564 unigenes and dinucleotide repeats represented the most abundant type. These findings have laid the foundation for further understanding the secretary processes and immune functions of loach skin mucus.

show abstract

Skin‐specific expression of ictacalcin, a homolog of the S100 genes, during zebrafish embryogenesis

Cited by 18 publications

References 26 publications

Structural and functional diversification in the teleost S100 family of calcium-binding proteins

Structural and functional diversification in the teleost S100 family of calcium-binding proteins

Epidermal transient down‐regulation of retinol‐binding protein 4 and mirror expression of apolipoprotein Eb and estrogen receptor 2a during zebrafish fin and scale development

De Novo Assembly of Mud Loach (Misgurnus anguillicaudatus) Skin Transcriptome to Identify Putative Genes Involved in Immunity and Epidermal Mucus Secretion

Contact Info

Product

Resources

About