Skin as the site of vitamin D synthesis and target tissue for 1,25-dihydroxyvitamin D3. Use of calcitriol (1,25-dihydroxyvitamin D3) for treatment of psoriasis

Holick, Michael F.

doi:10.1001/archderm.123.12.1677

Cited by 54 publications

(49 citation statements)

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“…The production of Vitamin D 3 begins from plasma membrane of skin cells where 7-dehydrocholesterol is photolyzed by UV light to produce previtamin D 3 which undergoes thermal isomerization to Vitamin D3. The synthesis of previtamin D 3 and Vitamin D 3 are self-controlled processes since further absorption of UV light causes isomerizations of these compounds to yield inactive products [4]. Conversion of 7-dehydrocholesterol to previtamin D 3 is decreased to less than half in elderly people [5].…”

Section: Introductionmentioning

confidence: 99%

The role of Vitamin D3 metabolism in prostate cancer

Lou

Qiao

Talonpoika

et al. 2004

The Journal of Steroid Biochemistry and Molecular Biology

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Section: Introductionmentioning

confidence: 99%

The role of Vitamin D3 metabolism in prostate cancer

Lou

Qiao

Talonpoika

et al. 2004

The Journal of Steroid Biochemistry and Molecular Biology

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“…Recently, receptors and actions for this hormone have been found in many tissues in addition to these traditional targets, including heart, brain, breast, and many tumor cells (6)(7)(8)(9)(10)(11)(12)(13). These novel actions of 1,25-(OH)2D3 may have significant physiologic, pathologic, and even therapeutic implications.…”

mentioning

confidence: 99%

“…These novel actions of 1,25-(OH)2D3 may have significant physiologic, pathologic, and even therapeutic implications. For example, 1,25-0H)2D3 induces differentiation of keratinocytes (6) and is useful in the treatment of psoriasis (7). 1,25-0H)2D3 also induces differentiation and inhibits DNA synthesis in HL-60 human promyelocytic leukemia cells (8,9), mouse M1-60 myeloid leukemia cells (10), and human U-937 lymphoma cells (11).…”

mentioning

confidence: 99%

1,25-dihydroxyvitamin D3 modulates growth of vascular smooth muscle cells.

Mitsuhashi¹,

Morris²,

Ives³

1991

J. Clin. Invest.

167

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We examined the effects of 1,25-dihydroxyvitamin D3(1,25-(OH)2D3) on the proliferation of vascular smooth muscle (VSM) cells. Receptors for 1,250-H)2D3 were demonstrated in fresh rabbit aortic tissue and in cultured rat VSM using binding of 13H1-1,25-0H)2D3 in sucrose density gradients of the tissue or cell homogenates. The receptor sedimented at 3.6 S, the sedimentation velocity of 1,2501H)2D3 receptors from other sources. 1,2540H)2D3 dramatically altered the growth of VSM, but this effect depended importantly on the basal conditions in which the cells were grown. In quiescent VSM deprived of serum for 72 h, 1,2540H)2D3 (0.1-10 nM), but not 25-(OH)D3 (up to 100 nM) increased thymidine incorporation up to 12-fold and cell number up to 2.6-fold compared with controls. The maximal effect of 1,25-(0H)D3 on thymidine incorporation was similar to the maximal effect of the growth factors a-thrombin or PDGF. Furthermore, the effects of 1,25-(OH)2D3 and thrombin on thymidine incorporation in quiescent cells were markedly synergistic, yielding a 78-fold increase in thymidine incorporation when both agents were added simultaneously. In "nonquiescent cells" which were exposed to serumfree medium for only 24 h, 1,2540H)2D3 (10 nM) also increased DNA synthesis 10-fold compared with controls. However, in striking contrast to what was observed in quiescent cells, 1,25-(OH)2D3 diminished the mitogenic response to thrombin by as much as 50% in nonquiescent cells. 1,25-(OHh)D3 also modulated the transcription of c-myc in response to thrombin. In quiescent cells, transcription was enhanced by 1,25-(OH)2D3, whereas in nonquiescent cells, thrombin-induced c-myc transcription was blunted. Thus, 1,25-(0H)2D3 is a potent modulator of the growth of cultured VSM. The direction of this modulation depends strongly on the conditions under which the cells are cultured. (J. Clin. Invest. 1991.

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“…For the past several years our laboratory has been conducting biochemical and clinical studies to determine the effects of 1,25(OH)2D3 on human skin, as well as developing 1,25(OH)2D3 as a therapeutic agent for treating hyperproliferative skin disorders such as psoraisis [Holick et al, 1987;Smith et al, 19881. These studies often involved supraphysiological doses of 1,25(OH),D3 with potential side effects.…”

Section: Resultsmentioning

confidence: 99%

Metabolism of ³H‐1α,25‐dihydroxyvitamin D₃ in cultured human keratinocytes

Ray

Holick

1995

J of Cellular Biochemistry

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In the present investigation we studied the metabolism of 1 alpha,25-dihydroxy-[1 beta-3H]vitamin D3 (3H-1,25(OH)2D3) in culture-grown human keratinocytes (CHK). Our results showed that the cellular uptake of 3H-1,25(OH)2D3, upon incubation with CHK, occurred very rapidly; and it paralleled a decrease in the concentration of 3H-1,25(OH)2D3 in the medium. The amount of 3H-calcitroic acid, on the other hand, increased slowly in the medium, while the concentration of 3H-calcitroic acid in the cell remained undetectable during the whole period of incubation. When the cells were preincubated with 1,25(OH)2D3 (10(-8)M), conversion of 3H-1,25(OH)2D3 to 3H-calcitroic acid increased almost twofold, indicating that 1,25(OH)2D3 catalyzed its own catabolism.

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Skin as the site of vitamin D synthesis and target tissue for 1,25-dihydroxyvitamin D3. Use of calcitriol (1,25-dihydroxyvitamin D3) for treatment of psoriasis

Cited by 54 publications

References 0 publications

The role of Vitamin D3 metabolism in prostate cancer

The role of Vitamin D3 metabolism in prostate cancer

1,25-dihydroxyvitamin D3 modulates growth of vascular smooth muscle cells.

Metabolism of ³H‐1α,25‐dihydroxyvitamin D₃ in cultured human keratinocytes

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Skin as the site of vitamin D synthesis and target tissue for 1,25-dihydroxyvitamin D3. Use of calcitriol (1,25-dihydroxyvitamin D3) for treatment of psoriasis

Cited by 54 publications

References 0 publications

The role of Vitamin D3 metabolism in prostate cancer

The role of Vitamin D3 metabolism in prostate cancer

1,25-dihydroxyvitamin D3 modulates growth of vascular smooth muscle cells.

Metabolism of 3H‐1α,25‐dihydroxyvitamin D3 in cultured human keratinocytes

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Metabolism of ³H‐1α,25‐dihydroxyvitamin D₃ in cultured human keratinocytes