Skeletal myogenic differentiation of human urine-derived cells as a potential source for skeletal muscle regeneration

Chen, Wei; Xie, Minkai; Yang, Bin; Bharadwaj, Shantaram; Song, Lujie; Liu, Guihua; Yi, Shanhong; Ye, Gang; Atala, Anthony; Zhang, Yuanyuan

doi:10.1002/term.1914

Cited by 27 publications

(10 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, adult stem cells, such as MSCs are a suitable source of cells for stem cell therapy, and may be used to promote the regeneration of damaged SKM. In a number of studies, stem cells derived from bone marrow, adipose tissue, the placenta, amniotic fluid, umbilical cord blood and urine have shown potential to promote SKM regeneration ( 1 , 11 – 14 , 18 ). However, the degree of regeneration obtained when using these cells may be insufficient when the injury or damage is severe ( 1 , 11 – 14 , 18 ).…”

Section: Discussionmentioning

confidence: 99%

“…A number of studies have been performed using stem cells in order to treat muscle-related diseases in which the organization of muscle tissue is adversely affected by congenital hereditary muscle defects, loss of muscle mass, trauma or tumor removal ( 1 – 5 ). Previous studies have reported the transplantation of muscle stem cell-derived myoblasts or myogenic cells in models of muscle injury ( 6 – 8 ).…”

Section: Introductionmentioning

confidence: 99%

“…However, there are major obstacles to the clinical use of ES and iPS cells, including teratoma formation and the rejection of transplanted cells by the immune system. Compared with these cells, mesenchymal stem cells (MSCs), which have the ability to regulate the immune system and do not form teratomas, may be isolated from various sources, including bone marrow ( 11 ), adipose tissue ( 12 ), umbilical cord blood ( 13 ), amniotic fluid ( 14 ), the placenta ( 15 ), dental pulp ( 16 ), the tonsils ( 17 ) and urine ( 1 ). For these reasons, MSCs have been recognized as a valuable source of cells which may be used to propagate myogenic cells according to the protocols for differentiation.…”

Section: Introductionmentioning

confidence: 99%

“…For these reasons, MSCs have been recognized as a valuable source of cells which may be used to propagate myogenic cells according to the protocols for differentiation. Several studies examining the differentiation of MSCs into myogenic cells have been reported the use of MSCs derived from adipose tissue, bone marrow, the placenta, amniotic fluid, umbilical cord blood and urine ( 1 , 2 , 11 – 14 , 18 ).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration

Park

Choi

Jung

et al. 2016

International Journal of Molecular Medicine

View full text Add to dashboard Cite

Stem cells are regarded as an important source of cells which may be used to promote the regeneration of skeletal muscle (SKM) which has been damaged due to defects in the organization of muscle tissue caused by congenital diseases, trauma or tumor removal. In particular, mesenchymal stem cells (MSCs), which require less invasive harvesting techniques, represent a valuable source of cells for stem cell therapy. In the present study, we demonstrated that human tonsil-derived MSCs (T-MSCs) may differentiate into myogenic cells in vitro and that the transplantation of myoblasts and myocytes generated from human T-MSCs mediates the recovery of muscle function in vivo. In order to induce myogenic differentiation, the T-MSC-derived spheres were cultured in Dulbecco's modified Eagle's medium/nutrient mixture F-12 (DMEM/F-12) supplemented with 1 ng/ml transforming growth factor-β, non-essential amino acids and insulin-transferrin-selenium for 4 days followed by culture in myogenic induction medium [low-glucose DMEM containing 2% fetal bovine serum (FBS) and 10 ng/ml insulin-like growth factor 1 (IGF1)] for 14 days. The T-MSCs sequentially differentiated into myoblasts and skeletal myocytes, as evidenced by the increased expression of skeletal myogenesis-related markers [including α-actinin, troponin I type 1 (TNNI1) and myogenin] and the formation of myotubes in vitro. The in situ transplantation of T-MSCs into mice with a partial myectomy of the right gastrocnemius muscle enhanced muscle function, as demonstrated by gait assessment (footprint analysis), and restored the shape of SKM without forming teratomas. Thus, T-MSCs may differentiate into myogenic cells and effectively regenerate SKM following injury. These results demonstrate the therapeutic potential of T-MSCs to promote SKM regeneration following injury.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration

Park

Choi

Jung

et al. 2016

International Journal of Molecular Medicine

View full text Add to dashboard Cite

show abstract

“…Hydrogels are also excellent carriers for drug loading and allow long time and spatio-temporal drug releases [158,159]. In one study, Liu et al differentiated human urine-derived stem cells (USCs) into a myogenic lineage by culturing them on alginate microbeads encapsulating a cocktail of growth factors and then subcutaneously engrafted these cells into nude mice [97,160]. Hydrogels can also be designed to be injectable.…”

Section: Nanofiber Scaffoldsmentioning

confidence: 99%

Stem Cell Differentiation Toward the Myogenic Lineage for Muscle Tissue Regeneration: A Focus on Muscular Dystrophy

Ostrovidov

Shi

Sadeghian

et al. 2015

Stem Cell Rev and Rep

View full text Add to dashboard Cite

Skeletal muscle tissue engineering is one of the important ways for regenerating functionally defective muscles. Among the myopathies, the Duchenne muscular dystrophy (DMD) is a progressive disease due to mutations of the dystrophin gene leading to progressive myofiber degeneration with severe symptoms. Although current therapies in muscular dystrophy are still very challenging, important progress has been made in materials science and in cellular technologies with the use of stem cells. It is therefore useful to review these advances and the results obtained in a clinical point of view. This article focuses on the differentiation of stem cells into myoblasts, and their application in muscular dystrophy. After an overview of the different stem cells that can be induced to differentiate into the myogenic lineage, we introduce scaffolding materials used for muscular tissue engineering. We then described some widely used methods to differentiate different types of stem cell into myoblasts. We highlight recent insights obtained in therapies for muscular dystrophy. Finally, we conclude with a discussion on stem cell technology. We discussed in parallel the benefits brought by the evolution of the materials and by the expansion of cell sources which can differentiate into myoblasts. We also discussed on future challenges for clinical applications and how to accelerate the translation from the research to the clinic in the frame of DMD.

show abstract

Quantitative Evaluation of Exon Skipping in Urine-Derived Cells for Duchenne Muscular Dystrophy

Kunitake

Sathyaprakash

Matsuki

et al. 2022

Methods in Molecular Biology

View full text Add to dashboard Cite

Skeletal myogenic differentiation of human urine-derived cells as a potential source for skeletal muscle regeneration

Cited by 27 publications

References 36 publications

Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration

Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration

Stem Cell Differentiation Toward the Myogenic Lineage for Muscle Tissue Regeneration: A Focus on Muscular Dystrophy

Quantitative Evaluation of Exon Skipping in Urine-Derived Cells for Duchenne Muscular Dystrophy

Contact Info

Product

Resources

About