Size-dependent cellular uptake of exosomes

Caponnetto, Federica; Manini, Ivana; Škrap, Miran; Pálmai-Pallag, Timea; Loreto, Carla Di; Beltrami, Antonio Paolo; Cesselli, Daniela; Ferrari, Enrico

doi:10.1016/j.nano.2016.12.009

Cited by 111 publications

(94 citation statements)

References 37 publications

(45 reference statements)

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“…These findings are supported by previous studies, which demonstrated stoichiometric differences in microRNA contents among different EV populations and between different methods. 44–46 These differences in micro-RNA expression profiles can be attributed to the different separation mechanisms used by the two methods, which result in nonidentical EV populations, as UC separates EVs based on density while ExoTIC separates EVs based on size .…”

Section: Resultsmentioning

confidence: 99%

The Exosome Total Isolation Chip

et al. 2017

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Circulating tumor-derived extracellular vesicles (EVs) have emerged as a promising source for identifying cancer biomarkers for early cancer detection. However, the clinical utility of EVs has thus far been limited by the fact that most EV isolation methods are tedious, nonstandardized, and require bulky instrumentation such as ultracentrifugation (UC). Here, we report a size-based EV isolation tool called ExoTIC (exosome total isolation chip), which is simple, easy-to-use, modular, and facilitates high-yield and high-purity EV isolation from biofluids. ExoTIC achieves an EV yield ~4–1000-fold higher than that with UC, and EV-derived protein and microRNA levels are well-correlated between the two methods. Moreover, we demonstrate that ExoTIC is a modular platform that can sort a heterogeneous population of cancer cell line EVs based on size. Further, we utilize ExoTIC to isolate EVs from cancer patient clinical samples, including plasma, urine, and lavage, demonstrating the device’s broad applicability to cancers and other diseases. Finally, the ability of ExoTIC to efficiently isolate EVs from small sample volumes opens up avenues for preclinical studies in small animal tumor models and for point-of-care EV-based clinical testing from fingerprick quantities (10–100 μL) of blood.

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Section: Resultsmentioning

confidence: 99%

The Exosome Total Isolation Chip

et al. 2017

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“…Compared to commercial technologies, the present one has been found to be two folds to 20 folds higher performance in EV isolation. Third, it can be implemented as a size-selective isolation of EVs, which is getting important since EV sizes correlate with secreted cell type [35].…”

Section: Discussionmentioning

confidence: 99%

Rapid and Efficient Isolation of Exosomes by Clustering and Scattering

Kim

Lee

Park

et al. 2020

JCM

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Tumor-derived extracellular vesicles (EVs) have become important biomarkers of liquid biopsies for precision medicine. However, the clinical application of EVs has been limited due to the lack of EV isolation practical technology applicable to clinical environments. Here, we report an innovative EV isolation method, which is quick and simple, and facilitates high-yield and high-purity EV isolation from blood. Introducing a cationic polymer in plasma resulted in rapid clustering of anionic EVs and a chaotropic agent can separate EVs from these clusters. Isolated EVs were characterized in terms of size distribution, morphology, surface protein markers, and exosomal RNA. Through performance comparison with various methods, including ultracentrifugation (UC), the present method delivered the highest recovery rate (~20 folds that of UC) and purity ratio (3.5 folds that of UC) of EVs in a short period of time (<20 min). The proposed method is expected to be used in basic and applied research on EV isolation and in clinical applications.

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“…The morphology and size of EVs are critical to determining their physiological role, distribution and concentration in the organ (Caponnetto et al, 2017;Reddy et al, 2007;Wu, Deng, & Klinke, 2015 F I G U R E 2 Biogenesis and content of exosomes. (a) Exosome contents and a schematic of some common proteins are shown.…”

Section: Changes In Morphology and Sizementioning

confidence: 99%

“…EVs are getting smaller and smaller, and their particle sizes are becoming more and more heterogeneous (Caponnetto et al, 2017).…”

mentioning

confidence: 99%

How does temperature play a role in the storage of extracellular vesicles?

Qin

Zhang

et al. 2020

Journal Cellular Physiology

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Extracellular vesicles (EVs) contain specific proteins, lipids, and nucleic acids that can be passed to other cells as signal molecules to alter their function. However, there are many problems and challenges in the conversion and clinical application of EVs. Storage and protection of EVs is one of the issues that need further research. To adapt to potential clinical applications, this type of problem must be solved. This review summarizes the storage practices of EVs in recent years, and explains the impact of temperature on the quality and stability of EVs during storage based on current research, and explains the potential mechanisms involved in this effect as much as possible.

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Size-dependent cellular uptake of exosomes

Cited by 111 publications

References 37 publications

The Exosome Total Isolation Chip

The Exosome Total Isolation Chip

Rapid and Efficient Isolation of Exosomes by Clustering and Scattering

How does temperature play a role in the storage of extracellular vesicles?

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