Size controlled protein nanoemulsions for active targeting of folate receptor positive cells

Abstract

Bovine serum albumin (BSA) nanoemulsions were produced by high pressure homogenization with a tri-block copolymer (Poloxamer 407), which presents a central hydrophobic chain of polyoxypropylene (PPO) and two identical lateral hydrophilic chains of polyethylene glycol (PEG). We observed a linear correlation between tri-block copolymer concentration and size - the use of 5mg/mL of Poloxamer 407 yields nanoemulsions smaller than 100nm. Molecular dynamics and fluorescent tagging of the tri-block copolymer highlight their mechanistic role on the size of emulsions. This novel method enables the fabrication of highly stable albumin emulsions in the nano-size range, highly desirable for controlled drug delivery. Folic Acid (FA)-tagged protein nanoemulsions were shown to promote specific folate receptor (FR)-mediated targeting in FR positive cells. The novel strategy presented here enables the construction of size controlled, functionalized protein-based nanoemulsions with excellent characteristics for active targeting in cancer therapy.

Keratin‐based particles for protection and restoration of hair properties

“…, it was possible to verify the particles’ spherical shape and the presence of a less dense layer surrounding the particles. This layer can be attributed to the presence of Pol 407 , which tends to accumulate in the interface between particles and water .…”

Oral nanoemulsions of candesartan cilexetil: formulation, characterization and in vitro drug release studies

“…The results are given in (Table 9) imply that there were no obvious changes in droplet size distribution, PDI, zeta potential and other physiochemical properties. It could be judged from the results gained, that poloxamer 407 adsorb strongly onto the surface of the nanodroplet via their hydrophobic polyoxypropylene center block and have been shown to be quite successful in regard to stabilization of NE droplet (Loureiro et al 2015). Additionally, expiration date of CC loaded NE-4 was found to be (2.589 years).…”

Synthesis of multifunctional bovine serum albumin microcapsules by the sonochemical method for targeted drug delivery and controlled drug release

“…So far, many techniques or methods have been designed to fabricate protein MCs, especially bovine serum albumin (BSA) MCs [6][7][8]. Among these techniques, sonochemical method is a simple physicochemical approach for effectively yielding stable and biocompatible protein MCs [9][10][11].…”