Six widespread bacterial clones among Escherichia coli K1 isolates

Achtman, Mark; Mercer, Andrew A.; Kušećek, Barica; Pohl, A; Heuzenroeder, Michael W.; Aaronson, W; Sutton, Ann; Silver, Richard P.

doi:10.1128/iai.39.1.315-335.1983

Cited by 423 publications

(214 citation statements)

References 34 publications

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“…Di¡erences with regard to the occurrence of some characteristics have already been observed. For example O'Brien et al demonstrated a large percentage of examined strains producing colicins (57.3%) [7] compared with isolates from Europe (21.6%) and the USA (33.3%) [23], while we have demonstrated 31% for strains from patients without infection and 44% for strains from intestinal infections.…”

Section: Discussioncontrasting

confidence: 44%

Virulence determinants of uropathogenic Escherichia coli in fecal strains from intestinal infections and healthy individuals

Kuhar¹

1998

FEMS Microbiology Letters

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Twenty-five fecal Escherichia coli strains of serogroups O6 and O18 from patients with and without intestinal infections were analyzed for fimbrial adhesins pap, prs and sfa, hemolysin, cytonecrotic factor, colicins, capsules, antibiotic resistances, plasmid content and some plasmid encoded characteristics. A high percentage of strains expressing P fimbriae was found with an even higher percentage in strains isolated from intestinal infections. A correlation was found between colicinogenicity and P fimbriae production. None of the strains produced hemolysin while 28% had cytonecrotic factor type 1 sequences, demonstrating that cytonecrotic factor type 1 is not always associated with hemolysin production and indicating that the examined strains do not harbor a larger pathogenicity island. Plasmids and plasmid associated characteristics were more frequently associated with the O18 serogroup and chromosomal colicin V genes were found independently of plasmids. z

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Section: Discussioncontrasting

confidence: 44%

Virulence determinants of uropathogenic Escherichia coli in fecal strains from intestinal infections and healthy individuals

Kuhar¹

1998

FEMS Microbiology Letters

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“…OMP patterns have been shown to be closely associated with electrophoretic types and clones identified by MLEE in other species (Achtman et al, 1983;Musser et al, 1985Musser et al, , 1988Achtman and Pluschke, 1986;Kapur et al, 1992;Davies et al, 1997). The exclusive association of isolates of the less common capsular types with specific OMP-types provided evidence that OMP-types mark individual clonal groups of P. multocida (Achtman and Pluschke, 1986).…”

Section: Discussionmentioning

confidence: 95%

Diversity of avian Pasteurella multocida strains based on capsular PCR typing and variation of the OmpA and OmpH outer membrane proteins

Davies

MacCorquodale

Caffrey

2003

Veterinary Microbiology

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One hundred avian Pasteurella multocida isolates recovered from cases of fowl cholera and related infections in England and Wales over a 13-year period were characterised by capsular PCR typing and analysis of outer membrane protein (OMP) profiles. Sixty-eight percent of the strains were of capsular type A, 14% were type F, 5% were type D, 4% were type B and 9% were untypable. Nineteen distinct OMP profiles (OMP-types) were identified based mainly on molecular mass heterogeneity of the heat-modifiable (OmpA) and porin (OmpH) proteins. Fifty-six percent of the isolates were represented by 15 OMP-types, whereas 44% of the isolates were associated with four OMP-types. The extensive molecular mass heterogeneity of the OmpA and OmpH proteins supports previous findings that avian P. multocida strains are very diverse. Furthermore, the isolates studied were associated with different clinical symptoms and were recovered from a wide range of lesions and tissues. The high degree of strain diversity together with the wide variety of clinical symptoms suggest that certain avian strains of P. multocida are opportunistic pathogens of relatively low virulence. Strains of capsular types B, D and F, as well as the untypable isolates, were associated exclusively with specific OMP-types and represent distinct and widely disseminated clonal groups. These observations support the view that avian strains of P. multocida have a clonal population structure. Based on previous studies, the molecular mass heterogeneity of the OmpA and OmpH proteins might provide a selective advantage to P. multocida by generating antigenic variation.

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“…PII: S 0 3 7 8 -1 0 9 7 ( 0 1 ) 0 0 1 3 4 -3 S17-1 Vpir donor strain and tagged pUTmini-Tn5Km2 suicide vectors [1]. The E. coli K1 strains used in this study were RS218, serotype O18ac:K1:H7, a clinical isolate from a case of human newborn meningitis [7]; EV291, a spontaneous nalidixic acid-resistant derivative of RS218; and EV293, a spontaneous K1-speci¢c bacteriophage-resistant derivative of EV291 harboring a random insertion of a tagged mini-Tn5Km2 element. Loss of the EV293 capsule was con¢rmed by this strain's lack of reactivity against polysialic acid antibody [8].…”

Section: Methodsmentioning

confidence: 99%

Adaptation of signature-tagged mutagenesis to Escherichia coli K1 and the infant-rat model of invasive disease

González

2001

FEMS Microbiology Letters

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With the exception of the polysialic acid capsule (K1 antigen), little is known about other virulence factors needed for systemic infection by Escherichia coli K1, the leading cause of Gram-negative neonatal meningitis in humans. In this work, the functional genomics method of signature-tagged mutagenesis (STM) was adapted to E. coli K1 and the infant-rat model to identify non-capsule virulence genes. Validation of the method was demonstrated by the failure to recover a reconstructed acapsular mutant from bacterial pools used to systemically infect 5-day-old rats. Three new genes required for systemic disease were identified from a total of 192 mutants screened by STM (1.56% hit rate). Gut colonization, Southern blot hybridization, mixed-challenge infection, and DNA sequence analyses showed that the attenuating defects in the mutants were associated with transposon insertions in rfaL (O antigen ligase), dsbA (thiol:disulfide oxidoreductase), and a new gene, puvA (previously unidentified virulence gene A), with no known homologues. The results indicate the ability of STM to identify novel systemic virulence factors in E. coli K1. ß 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.

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Six widespread bacterial clones among Escherichia coli K1 isolates

Cited by 423 publications

References 34 publications

Virulence determinants of uropathogenic Escherichia coli in fecal strains from intestinal infections and healthy individuals

Virulence determinants of uropathogenic Escherichia coli in fecal strains from intestinal infections and healthy individuals

Diversity of avian Pasteurella multocida strains based on capsular PCR typing and variation of the OmpA and OmpH outer membrane proteins

Adaptation of signature-tagged mutagenesis to Escherichia coli K1 and the infant-rat model of invasive disease

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