Site-Specific Nitrosoproteomic Identification of Endogenously<i>S</i>-Nitrosylated Proteins in Arabidopsis

Hu, Jiliang; Huang, Xiahe; Chen, Lichao; Sun, Xuwu; Lu, Congming; Zhang, Lixin; Wang, Yingchun; Zuo, Jianru

doi:10.1104/pp.15.00026

Cited by 208 publications

(200 citation statements)

References 77 publications

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“…We reasoned that the NO-enhanced APX1 enzymatic activity might be partly attributed to S-nitrosylation. Consistent with this notion, APX1 was identified as a putative S-nitrosylated protein by nitrosoproteomic studies (Fares et al, 2011;Hu et al, 2015). By using the biotin-switch method (Jaffrey and Snyder, 2001), we found that APX1 recombinant protein was efficiently S-nitrosylated in vitro ( Fig.…”

Section: Apx1 Is a Positive Regulator Of No-modulated Resistance To Osupporting

confidence: 72%

“…S6). Moreover, this residue has also been identified to be S-nitrosylated in Arabidopsis by proteomics studies and in pea by a mass spectrometric study, respectively (Fares et al, 2011;Begara-Morales et al, 2014;Hu et al, 2015). The importance of Cys-32 is evident by the observation that soybean APX1 contains a single Cys residue (accession no.…”

Section: Discussionmentioning

confidence: 97%

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S-Nitrosylation Positively Regulates Ascorbate Peroxidase Activity during Plant Stress Responses

et al. 2015

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Nitric oxide (NO) and reactive oxygen species (ROS) are two classes of key signaling molecules involved in various developmental processes and stress responses in plants. The burst of NO and ROS triggered by various stimuli activates downstream signaling pathways to cope with abiotic and biotic stresses. Emerging evidence suggests that the interplay of NO and ROS plays a critical role in regulating stress responses. However, the underpinning molecular mechanism remains poorly understood. Here, we show that NO positively regulates the activity of the Arabidopsis (Arabidopsis thaliana) cytosolic ascorbate peroxidase1 (APX1). We found that S-nitrosylation of APX1 at cysteine (Cys)-32 enhances its enzymatic activity of scavenging hydrogen peroxide, leading to the increased resistance to oxidative stress, whereas a substitution mutation at Cys-32 causes the reduction of ascorbate peroxidase activity and abolishes its responsiveness to the NO-enhanced enzymatic activity. Moreover, S-nitrosylation of APX1 at Cys-32 also plays an important role in regulating immune responses. These findings illustrate a unique mechanism by which NO regulates hydrogen peroxide homeostasis in plants, thereby establishing a molecular link between NO and ROS signaling pathways.

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Section: Apx1 Is a Positive Regulator Of No-modulated Resistance To Osupporting

confidence: 72%

Section: Discussionmentioning

confidence: 97%

S-Nitrosylation Positively Regulates Ascorbate Peroxidase Activity during Plant Stress Responses

et al. 2015

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“…A large number of proteins regulating a range of metabolic and developmental processes are known to be targets of NO (Hu et al, 2015). Assuming that the observed shift is related to hyperaccumulation of NO in shr mutant, it is plausible that it may have affected the activity of several key proteins regulating cellular metabolism.…”

Section: Discussionmentioning

confidence: 99%

Nitric Oxide Overproduction in Tomato shr Mutant Shifts Metabolic Profiles and Suppresses Fruit Growth and Ripening

et al. 2016

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Nitric oxide (NO) plays a pivotal role in growth and disease resistance in plants. It also acts as a secondary messenger in signaling pathways for several plant hormones. Despite its clear role in regulating plant development, its role in fruit development is not known. In an earlier study, we described a short root (shr) mutant of tomato, whose phenotype results from hyperaccumulation of NO. The molecular mapping localized shr locus in 2.5 Mb region of chromosome 9. The shr mutant showed sluggish growth, with smaller leaves, flowers and was less fertile than wild type. The shr mutant also showed reduced fruit size and slower ripening of the fruits post-mature green stage to the red ripe stage. Comparison of the metabolite profiles of shr fruits with wild-type fruits during ripening revealed a significant shift in the patterns. In shr fruits intermediates of the tricarboxylic acid (TCA) cycle were differentially regulated than WT indicating NO affected the regulation of TCA cycle. The accumulation of several amino acids, particularly tyrosine, was higher, whereas most fatty acids were downregulated in shr fruits. Among the plant hormones at one or more stages of ripening, ethylene, Indole-3-acetic acid and Indole-3-butyric acid increased in shr, whereas abscisic acid declined. Our analyses indicate that the retardation of fruit growth and ripening in shr mutant likely results from the influence of NO on central carbon metabolism and endogenous phytohormones levels.

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“…ABA‐induced NO can modify the reactive cysteine thiol in a protein to form S‐nitrosothiol. Protein S‐nitrosylation is important for protein activity and stability (Hu et al ). OST1 activity is abolished by S‐nitrosylation at cysteine (Cys) 137, which is adjacent to the kinase catalytic site (Wang et al ), suggesting that NO regulates ABA signaling via a feedback circuit.…”

Section: Apoplastic H2o2 Mediates Stomatal Movement During Aba Signalingmentioning

confidence: 99%

Reactive oxygen species signaling and stomatal movement in plant responses to drought stress and pathogen attack

Song

Wang

et al. 2018

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450

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Stomata, the pores formed by a pair of guard cells, are the main gateways for water transpiration and photosynthetic CO exchange, as well as pathogen invasion in land plants. Guard cell movement is regulated by a combination of environmental factors, including water status, light, CO levels and pathogen attack, as well as endogenous signals, such as abscisic acid and apoplastic reactive oxygen species (ROS). Under abiotic and biotic stress conditions, extracellular ROS are mainly produced by plasma membrane-localized NADPH oxidases, whereas intracellular ROS are produced in multiple organelles. These ROS form a sophisticated cellular signaling network, with the accumulation of apoplastic ROS an early hallmark of stomatal movement. Here, we review recent progress in understanding the molecular mechanisms of the ROS signaling network, primarily during drought stress and pathogen attack. We summarize the roles of apoplastic ROS in regulating stomatal movement, ABA and CO signaling, and immunity responses. Finally, we discuss ROS accumulation and communication between organelles and cells. This information provides a conceptual framework for understanding how ROS signaling is integrated with various signaling pathways during plant responses to abiotic and biotic stress stimuli.

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Site-Specific Nitrosoproteomic Identification of EndogenouslyS-Nitrosylated Proteins in Arabidopsis

Cited by 208 publications

References 77 publications

S-Nitrosylation Positively Regulates Ascorbate Peroxidase Activity during Plant Stress Responses

S-Nitrosylation Positively Regulates Ascorbate Peroxidase Activity during Plant Stress Responses

Nitric Oxide Overproduction in Tomato shr Mutant Shifts Metabolic Profiles and Suppresses Fruit Growth and Ripening

Reactive oxygen species signaling and stomatal movement in plant responses to drought stress and pathogen attack

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