Sister chromatid exchange in murine alveolar macrophages, regenerating liver and bone marrow cells ? a simultaneous multicellular in vivo assay

Conner, Mary K.; Alarie, Yves; Dombroske, Rebecca L.

doi:10.1007/bf00344482

Cited by 29 publications

(8 citation statements)

References 6 publications

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“…1976;Vogel and Bauknecht. 1976;Allen et al, 1977) in order to screen for genotoxic effects in specific target tissues and organs (Allen et al 1976;Conner et al 1979: Kram et al 1979a. especial ly in circulating peripheral lymphocytes (Klingerman et al 1981;Crossen.…”

mentioning

confidence: 99%

A comparison of sister chromatid exchange frequencies in peripheral lymphocytes and bone marrow cells of Fischer 344 and Sprague-Dawley rats

Dearfield¹,

Jacobson‐Kram²,

Williams³

1984

Cytogenet Genome Res

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We have investigated spontaneous sister chromatid exchange (SCE) frequencies in peripheral lymphocytes and bone marrow cells explanted from two strains of the laboratory rat, Fischer 344 and Sprague-Dawley. A small, but significant difference was noted for both cell types, with the Fischer 344 rat being consistently higher. Other cell parameters, such as the mitotic index and the replicative index, were similar in the two strains. SCE levels in cultured peripheral lymphocytes after intraperitoneal administration of the alkylating drug cyclophosphamide (10 mg/kg) were similar for the two strains. Fischer 344 rats are known to have approximately a 10-fold higher incidence of spontaneous leukemia than do Sprague-Dawley rats. Since SCE frequency is a sensitive measure of DNA damage, our observations suggest that high leukemia incidence in the Fischer 344 rat may be related to a higher level of spontaneous DNA damage.

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mentioning

confidence: 99%

A comparison of sister chromatid exchange frequencies in peripheral lymphocytes and bone marrow cells of Fischer 344 and Sprague-Dawley rats

Dearfield¹,

Jacobson‐Kram²,

Williams³

1984

Cytogenet Genome Res

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“…A similar control SCE frequency level has been reported in Fisher 344 rat bone marrow cells in vitro [Dearfield et al, 19841. Also, nonsignificant differences of baseline SCE frequencies have been reported by Conner et al [1979] in in vivo mouse bone marrow, alveolar macrophage, and liver cells. In the present study, however, the Chinese hamster spleen cells had significantly lower SCE levels than bone marrow cells, and these levels were also lower than mouse spleen cells.…”

Section: Discussionmentioning

confidence: 91%

A comparison of baseline and cyclophosphamide‐induced sister chromatid exchanges in bone marrow and spleen cells of mouse and chinese hamster

et al. 1986

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Baseline sister chromatid exchange (SCE) frequencies were investigated in bone marrow and spleen cells of mice and Chinese hamsters. No significant difference in SCE frequency was noted for bone marrow in both species and for bone marrow and spleen in mice on per cell and per pg DNA basis. However, a significant difference was noted between species in spleen and between cell types in Chinese hamsters. Also, statistically significant differences were noted between species for both cell types when the same data were expressed on per chromosome basis. SCE levels in cultured bone marrow and spleen cells after intraperitoneal administration of the antineoplastic drug cyclophosphamide (10 and 20 mg/kg) differed significantly in mice and Chinese hamsters on per cell, per pg DNA content, and per chromosome basis. The spleen cells were much more sensitive to the effects of cyclophosphamide than bone marrow cells in both species. The replicative indices did not differ significantly between treated and control animals in either bone marrow or spleen cells of both species. Since SCE frequency is a sensitive measure of DNA damage, and bone marrow and lymphocytes are the most widely used cell types in human and animal in vivo assays, the methodologies and results reported here may be useful for comparative mammalian cytogenetic studies.

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“…cells in the normal rat. As these macrophages represent only 1 to 2 % ofthe total PAM, it is unlikely that they could contribute significantly in number to macrophages harvested from mechanically disrupted lung tissue (13). Table 3 shows the numbers of leukocytes in alveolar capillaries and in the whole circulating blood.…”

Section: Studies In Normal Rats Quantitative Analysis Of Lung Cellsmentioning

confidence: 99%

“…Quantitatively, the intraalveolar proliferation of PAM is not negligible; it produces several percent of new PAM per day (9)(10)(11)(12). Some experimental results under steady-state conditions have shown that most PAM could be directly derived from actively dividing cells (13), but also that PAM do not correspond to a self-renewing population (14). Thus, we have formulated the hypothesis that PAM proliferation is a maturation process of new precursors migrating to the endoalveolar compartment (15).…”

Section: Introductionmentioning

confidence: 99%

Overview of pulmonary alveolar macrophage renewal in normal rats and during different pathological processes.

Fritsch

Massé

1992

Environ Health Perspect

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We& report experimental results on pulmonary alveolar macrophage (PAM) renewal in healthy rats and in rats treated with particles introduced in the lungs. Morphometric studies showed that the lungs of normal rats of the strain used in our study contain 20 x 10' PAM, 50 x 10' monocytes in alveolar capillaries, and about 3 x 10' interstitial macrophages.Pulse labeling with a tritiated thymidine (3HI) gave a labeling index of OA% for the monocytes, of which a few could be observed in mitosis within alveolar capiHlaries. These monocywes are ikely to be the PAM presos The

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Sister chromatid exchange in murine alveolar macrophages, regenerating liver and bone marrow cells ? a simultaneous multicellular in vivo assay

Cited by 29 publications

References 6 publications

A comparison of sister chromatid exchange frequencies in peripheral lymphocytes and bone marrow cells of Fischer 344 and Sprague-Dawley rats

A comparison of sister chromatid exchange frequencies in peripheral lymphocytes and bone marrow cells of Fischer 344 and Sprague-Dawley rats

A comparison of baseline and cyclophosphamide‐induced sister chromatid exchanges in bone marrow and spleen cells of mouse and chinese hamster

Overview of pulmonary alveolar macrophage renewal in normal rats and during different pathological processes.

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