2015
DOI: 10.4161/15384101.2014.980641
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Abstract: In principle, a decline in base excision repair (BER) efficiency with age should lead to genomic instability and ultimately contribute to the onset of the aging phenotype. Although multiple studies have indicated a negative link between aging and BER, the change of BER efficiency with age in humans has not been systematically analyzed. Here, with foreskin fibroblasts isolated from 19 donors between 20 and 64 y of age, we report a significant decline of BER efficiency with age using a newly developed GFP reacti… Show more

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Cited by 93 publications
(90 citation statements)
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“…SIRT6, an NAD ϩ -dependent histone/ protein deacetylase and mono-ADP-ribosyltransferase, targets H3K56 for deacetylation, and ADP-ribosylates PARP1 to promote recruitment of downstream BER factors (58). SIRT6 deficiency leads to a phenotype that is consistent with BER defects (sensitivity to MMS, H 2 O 2 , and ionizing radiation) (40).…”
Section: Tablementioning
confidence: 93%
“…SIRT6, an NAD ϩ -dependent histone/ protein deacetylase and mono-ADP-ribosyltransferase, targets H3K56 for deacetylation, and ADP-ribosylates PARP1 to promote recruitment of downstream BER factors (58). SIRT6 deficiency leads to a phenotype that is consistent with BER defects (sensitivity to MMS, H 2 O 2 , and ionizing radiation) (40).…”
Section: Tablementioning
confidence: 93%
“…To test the influence of aging on DNA DSB repair in humans, we first isolated 50 fibroblast cell lines from the eyelid tissue of female donors at the age of 16-75 years according to our wellestablished protocol (Table 1). 18,19 The isolated fibroblasts were cultured in proper conditions to minimize potentially harmful stress to primary cells. In addition, to rule out the effect of replicative cellular senescence on DNA repair, all the following analysis in this report was performed at a population doubling (PD) number of [16][17][18][19] in all cell lines (Supplementary Table S1).…”
Section: Resultsmentioning
confidence: 99%
“…Eyelids were acquired from plastic surgeries and kept in ice-cold PBS; fibroblasts isolation experiments were performed within 24 h. The detailed isolation protocol is as previously reported. 18,19 Cell culture. All primary fibroblasts were cultured in MEM medium (Hyclone, Logan, UT, USA, Cat.…”
Section: Methodsmentioning
confidence: 99%
“…Acting through deacetylation of chromatin, SIRT6 may increase access of repair machinery to sites of DNA damage (169). Alternatively, SIRT6 may be indirectly promoting BER through activation of the PARP family of proteins (170,171). Double-strand break repair may also be mediated indirectly by increased PARP activity but also through non-histone deacetylation of CtIP (C-terminal binding protein interacting protein), a key protein involved in DSB end resection (172).…”
Section: Sirt6mentioning
confidence: 99%
“…Additionally, a few studies have reported the role of SIRT1 (151,153,154,165,166) and SIRT6 (169)(170)(171) in promoting DNA repair. These reports may offer support for my data, which demonstrated NR-mediated DNA repair efficiency in a partially SIRT1 and SIRT6 dependent manner.…”
Section: Nr-mediated Sirt Activation In Dna Damage and Repairmentioning
confidence: 99%