2013
DOI: 10.18632/aging.100542
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Abstract: Sirtuins are protein deacetylases used as therapeutic targets. Pharmacological Sirt1 activation has been questioned since the in vitro activator resveratrol failed to stimulate deacetylation of several physiological substrates. We tested the influence of substrate sequence by analyzing resveratrol effects on Sirt1-dependent deacetylation of 6802 physiological acetylation sites using peptide microarrays. Resveratrol stimulated deacetylation of a small set of sites and inhibited deacetylation of another set, whe… Show more

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Cited by 96 publications
(66 citation statements)
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References 18 publications
(20 reference statements)
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“…Resveratrol is also a SIRT1 activator (Frazzi et al 2013;Lakshminarasimhan et al 2013;Li et al 2013). When SIRT1 is activated, SIRT1 will block FOXO3 accumulation in the nucleus and inhibit cell death.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Resveratrol is also a SIRT1 activator (Frazzi et al 2013;Lakshminarasimhan et al 2013;Li et al 2013). When SIRT1 is activated, SIRT1 will block FOXO3 accumulation in the nucleus and inhibit cell death.…”
Section: Discussionmentioning
confidence: 99%
“…SIRT1 is a deacetylase that can remove the acetyl group from FOXO3 and NFκB (Frazzi et al 2013;Lakshminarasimhan et al 2013;Li et al 2013). …”
Section: Introductionmentioning
confidence: 99%
“…Recombinant human Sirt1, Sirt3, and Sirt5 were purified as described previously (12,45). Briefly, His-tagged full-length Sirt1 (in pET15b), His-Trxtagged Sirt3 residues 118-399 (in pVFT3S), and His-tagged Sirt5 residues 34-302 (in pET151/D-TOPO) were expressed in Escherichia coli BL21DE3 Rosetta2 (Novagen) and purified by affinity chromatography with Talon resin (Clontech).…”
Section: Methodsmentioning
confidence: 99%
“…However, several studies questioned the effectiveness of resveratrol and related compounds as direct SIRT1 activators, pointing out that the observed stimulatory effect depended on the presence of a fluorescent group in the substrates used in in vitro assays (Borra et al 2005;Kaeberlein et al 2005;Pacholec et al 2010). Nevertheless, it is possible that the artificial fluorescent group in the substrate peptide may mimic an aromatic residue in certain natural substrates of SIRT1 (Dai et al 2010;Hubbard et al 2013;Lakshminarasimhan et al 2013;Sinclair and Guarente 2014).…”
mentioning
confidence: 99%