Sinigrin in combination with artesunate provides protection against lethal murine malaria via falcipain-3 inhibition and immune modulation

Walter, Neha Sylvia; Gorki, Varun; Chauhan, Monika; Dhingra, Neelima; Kaur, Sukhbir

doi:10.1016/j.intimp.2021.108320

Cited by 6 publications

(3 citation statements)

References 44 publications

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“…This observation demonstrated the possible synergistic effect between the combination of artesunate and crude extracts from Prabchompoothaweep remedy, as artesunate is presented to be rapid absorbed in the tissues and quickly excreted after administration. Furthermore, previous study suggested that the crude extracts might aim to enhance the bioactivity of artesunate and the permeability of the drug in infected red blood cells [ 20 ]. This leads to the rapid clearance of parasites with no recrudescence in the early phase of infection, whereas a slow clearance of parasites with recrudescence was observed in monotherapy.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, artesunate combined with tetramethylpyrazine, which is a component of the Chuanxiong showed potential adjuvant therapy against ECM by reducing microvascular blockage and improving neurological function [ 19 ]. Furthermore, the combination treatment of sinigrin and artesunate demonstrated parasitemia suppression with no recrudescence and enhanced survival in P. berghei infected mice [ 20 ]. Therefore, plants may be used as adjuvant therapy with additive effects with artesunate or its derivatives in the treatment of cerebral malaria in order to combat its pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of artesunate combined with Atractylodes lancea or Prabchompoothaweep remedy extracts as adjunctive therapy for the treatment of cerebral malaria

Plirat,

Chaniad,

Phuwajaroanpong

et al. 2023

BMC Complement Med Ther

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Background Cerebral malaria is one of the most serious complications of Plasmodium infection and causes behavioral changes. However, current antimalarial drugs have shown poor outcomes. Therefore, new antimalarials with neuroprotective effects are urgently needed. This study aimed to evaluate the effects of selected extracts as monotherapy or adjunctive therapy with artesunate on antimalarial, anti-inflammatory, antioxidant, and neuroprotective properties in experimental cerebral malaria (ECM). Methods ECM was induced in male C57BL/6 mice by infection with Plasmodium berghei ANKA (PbA). Ethanolic extracts of Atractylodes lancea (a dose of 400 mg/kg) and Prabchompoothaweep remedy (a dose of 600 mg/kg) were evaluated as monotherapy and adjunctive therapy combined with artesunate at the onset of signs of cerebral malaria and continued for 7 consecutive days. Parasitemia, clinical scores, and body weight were recorded throughout the study. At day 13 post-infection, mouse brains were dissected and processed for the study of the inflammatory response, oxidative stress, blood–brain barrier (BBB) integrity, histopathological changes, and neurocognitive impairments. Results Ethanolic extracts of A. lancea and Prabchompoothaweep remedy alone improved cerebral malaria outcome in ECM, whereas artesunate combined with extracts of A. lancea or Prabchompoothaweep remedy significantly improved the outcome of artesunate and crude extracts alone. Using real-time PCR, PbA-infected mice that had received the combination treatment showed significantly reduced gene expression of inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-10), chemokines (CXCL4 and CXCL10), and adhesion molecules (ICAM-1, VCAM1, and CD36). The PbA-infected mice that received the combination treatment showed a significantly decreased malondialdehyde level compared to the untreated group. Similarly, the Evans blue dye assay revealed significantly less dye extravasation in the brains of infected mice administered the combination treatment, indicating improved BBB integrity. Combination treatment improved survival and reduced pathology in the PbA-infected group. Additionally, combination treatment resulted in a significantly reduced level of cognitive impairment, which was analyzed using a novel object recognition test. Conclusions This study demonstrated that artesunate combined with A. lancea or Prabchompoothaweep remedy extracts as adjunctive therapy reduced mortality, neuroinflammation, oxidative stress, BBB integrity protection, and neurocognitive impairment in the ECM.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficacy of artesunate combined with Atractylodes lancea or Prabchompoothaweep remedy extracts as adjunctive therapy for the treatment of cerebral malaria

Plirat,

Chaniad,

Phuwajaroanpong

et al. 2023

BMC Complement Med Ther

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“…Melrose in 2019 illustrated sinigrin uses and benefits in biomedicine 4 . Previous studies showed Sinigrin's role in modulating the immune system 7 . Therefore, the present study aims to evaluate the potential anti-microbial activity of sinigrin secondary metabolite against different bacterial isolates, which may suggest the possibility of bringing it as an alternative treatment in the future.…”

Section: Introductionmentioning

confidence: 99%

Study on the anti-microbial effect of Sinigrin against some pathogenic bacterial species

Hasan

Ghafil

2022

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The increasing anti-bacterial drug resistance is one of the biggest challenges facing doctors around the globe, so finding alternative treatments is one of the ideal options to overcome this problem. The cruciferous family is one of the wealthiest plants worldwide because it contains the most important secondary metabolites, glucosinolates, known for their anti-microbial properties. The present study aimed to evaluate the anti-bacterial effect of glucosinolates (Sinigrin) against eight bacterial isolates (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Actinomyces, Proteus mirabilis and Streptococcus pneumoniae). The current study investigated six concentrations of pure Sinigrin (100, 300, 500, 700, 900, and 1100 µg/ml). The sensitivity of bacterial isolates to various antibiotics was tested by VITIK 2DensiCheck equipment. The anti-bacterial activity of Sinigrin was assessed using the agar diffusion method, and the microtiter plate method measured the minimal inhibitory concentration (MIC). The highest anti-bacterial effect of Sinigrin was observed against S. aureus, E. coli, and E. faecalis. The anti-bacterial activity started as lower as 100 µg/ml, while a moderate effect was seen against P. aeruginosa and K. pneumoniae at a concentration lower than 700 µg/ml. On the other hand, Sinigrin was not effective against Actinomyces, P. mirabilis, and S. pneumoniae. It can be concluded from the present study that Sinigrin has an anti-bacterial effect on some isolates of bacteria which suggests the possibility of using Sinigrin as alternative medicine in the future. Keywords: Anti-bacterial activity, Agar well diffusion, Glucosinolates, Minimum inhibition concentration and antibiotic susceptibility, Sinigrin.

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Synergistic Herb-Drug Interactions with Antifungal and Antiparasitic Agents

Hemaiswarya

Prabhakar

Doble

2022

Herb-Drug Combinations

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Sinigrin in combination with artesunate provides protection against lethal murine malaria via falcipain-3 inhibition and immune modulation

Cited by 6 publications

References 44 publications

Efficacy of artesunate combined with Atractylodes lancea or Prabchompoothaweep remedy extracts as adjunctive therapy for the treatment of cerebral malaria

Efficacy of artesunate combined with Atractylodes lancea or Prabchompoothaweep remedy extracts as adjunctive therapy for the treatment of cerebral malaria

Study on the anti-microbial effect of Sinigrin against some pathogenic bacterial species

Synergistic Herb-Drug Interactions with Antifungal and Antiparasitic Agents

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