Single-stranded DNA aptamers that bind differentiated but not parental cells: subtractive systematic evolution of ligands by exponential enrichment

Wang, Chenglong; Zhang, Ming; Yang, Guang; Zhang, Dajing; Ding, Hongmei; Wang, Huixing; Fan, Ming Z.; Shen, Beifen; Shao, Ningsheng

doi:10.1016/s0168-1656(02)00360-7

Cited by 125 publications

(69 citation statements)

References 28 publications

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“…Most aptamers reported so far have been selected by using simple targets, such as a purified protein. Recently, aptamer selection against complex targets, such as red blood cell membranes and endothelial cells, was also demonstrated (13)(14)(15)(16). Compared with molecular probes currently available for biomarker recognition, aptamers are emerging candidates with ample potential due to their high specificity, low molecular weight, easy and reproducible production, versatility in application, and easy discovery and manipulation (17).…”

mentioning

confidence: 99%

Aptamers evolved from live cells as effective molecular probes for cancer study

Shangguan

Liu

Tang

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

1,347

1,255

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Using cell-based aptamer selection, we have developed a strategy to use the differences at the molecular level between any two types of cells for the identification of molecular signatures on the surface of targeted cells. A group of aptamers have been generated for the specific recognition of leukemia cells. The selected aptamers can bind to target cells with an equilibrium dissociation constant (Kd) in the nanomolar-to-picomolar range. The cell-based selection process is simple, fast, straightforward, and reproducible, and, most importantly, can be done without prior knowledge of target molecules. The selected aptamers can specifically recognize target leukemia cells mixed with normal human bone marrow aspirates and can also identify cancer cells closely related to the target cell line in real clinical specimens. The cell-based aptamer selection holds a great promise in developing specific molecular probes for cancer diagnosis and cancer biomarker discovery.cell-based selection ͉ cell imaging ͉ DNA aptamers

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mentioning

confidence: 99%

Aptamers evolved from live cells as effective molecular probes for cancer study

Shangguan

Liu

Tang

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

1,347

1,255

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“…Living cells, which express the target receptors on their surfaces, are appropriate for binding target because receptors expressed on living cells, unlike receptors in membrane fractions, assume their native structure and display only their extracellular side. In fact, living cells were used to screen DNA or RNA aptamers (29)(30)(31)(32)(33)(34)(35), as well as both peptides and antibodies, using phage display libraries (36)(37)(38)(39)(40)(41), which bind to membrane proteins expressed on the cell surface. Nevertheless, selection with live cells has not been performed with in vitro display techniques because mRNA display and ribosome display (in which a peptide is displayed on mRNA) are labile and easily degraded by ribonucleases in serum-supplemented cell culture medium.…”

mentioning

confidence: 99%

In vitro selection of a peptide antagonist of growth hormone secretagogue receptor using cDNA display

Ueno

Yoshida

Mondal

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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G protein-coupled receptors (GPCRs) are major drug targets, and their ligands are currently being explored and developed by many pharmaceutical companies and independent researchers. Class A (rhodopsin-like) GPCRs compose a predominant GPCR family; therefore, class A GPCR ligands are in demand. Growth hormone secretagogue receptor (GHS-R) is a class A GPCR that stimulates food intake by binding to its peptide ligand, ghrelin. Therefore, antagonists of GHS-R are expected to exert antiobesity function. In this article, we describe the use of cDNA display to screen for successfully and identify an antagonistic peptide of GHS-R. The antagonistic peptide inhibited the ghrelin-induced increase in intracellular Ca 2þ in vitro (IC 50 ¼ approximately 10 μM) and repressed the contraction of isolated animal stomach in response to ghrelin. Furthermore, peripheral administration of the peptide inhibited the food intake of mice. This work provides new insight into the development of antiobesity drugs and describes a method for the discovery of unique peptide ligands for class A GPCRs.aptamer | in vitro display | peptide drug | ligand screening | cell-based selection

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“…͔; cancer biomarkers; [22][23][24][25] HIVassociated proteins; [26][27][28][29] food-borne pathogens; 30 and other biologically important biomolecules such as insulin, 31,32 immunoglobulin E ͑IgE͒, 33 and thrombin. 34 They exhibit protein binding affinities that are comparable to those of corresponding antibodies.…”

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confidence: 99%

Direct detection of aptamer-thrombin binding via surface-enhanced Raman spectroscopy

et al. 2010

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Abstract. In this study, we exploit the sensitivity offered by surfaceenhanced Raman scattering ͑SERS͒ for the direct detection of thrombin using the thrombin-binding aptamer ͑TBA͒ as molecular receptor. The technique utilizes immobilized silver nanoparticles that are functionalized with thiolated thrombin-specific binding aptamer, a 15-mer ͑5Ј-GGTTGGTGTGGTTGG-3Ј͒ quadruplex forming oligonucleotide. In addition to the Raman vibrational bands corresponding to the aptamer and blocking agent, new peaks ͑mainly at 1140, 1540, and 1635 cm −1 ͒ that are characteristic of the protein are observed upon binding of thrombin. These spectral changes are not observed when the aptamer-nanoparticle assembly is exposed to a nonbinding protein such as bovine serum albumin ͑BSA͒. This methodology could be further used for the development of label-free biosensors for direct detection of proteins and other molecules of interest for which aptamers are available.

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Single-stranded DNA aptamers that bind differentiated but not parental cells: subtractive systematic evolution of ligands by exponential enrichment

Cited by 125 publications

References 28 publications

Aptamers evolved from live cells as effective molecular probes for cancer study

Aptamers evolved from live cells as effective molecular probes for cancer study

In vitro selection of a peptide antagonist of growth hormone secretagogue receptor using cDNA display

Direct detection of aptamer-thrombin binding via surface-enhanced Raman spectroscopy

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