Single Protein Molecules Visualized and Tracked in the Interior of Eukaryotic Cells

Tracking and Analyzing the Brownian Motion of Nano-objects Inside Hollow Core Fibers

“…Although the structures of viruses or proteins cannot be directly imaged with regular microscopy, their positions can be dynamically determined with high accuracy by fluorescent localization microscopy. − Fluorescence imaging has good spatial resolution but also has limitations, such as the demand for (invasive) labeling, finite photon budget, and bleaching, which all can be unfavorable regarding applications. − …”

Tracking and Analyzing the Brownian Motion of Nano-objects Inside Hollow Core Fibers

“…Although the structures of viruses or proteins cannot be directly imaged with regular microscopy, their positions can be dynamically determined with high accuracy by fluorescent localization microscopy. − Fluorescence imaging has good spatial resolution but also has limitations, such as the demand for (invasive) labeling, finite photon budget, and bleaching, which all can be unfavorable regarding applications. − …”

Fluorophore Selection for Single‐Molecule Fluorescence Spectroscopy (SMFS) and Photobleaching Pathways

“…Thus, single-molecule fluorescence spectroscopy (SMFS) is used in a range of scientific areas in physics, chemistry, and biology, and has been explained in detail in various excellent reviews [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16].The ongoing success of SMFS has been facilitated not only through the development of optical single-molecule techniques, but in addition through new refined organic synthesis methods and the large repertoire of molecular biology techniques. From such an observation, you cannot answer questions about the individual routes that the trains took, how many passengers boarded the train at which station, and at what times, or how many stops, each train made.…”

Nanoscopic Medicine: The Next Frontier

“…16 Both techniques provide a lateral resolution of ≈220 nm and a vertical resolution of ≈600 nm, thus allowing thick samples to be sectioned optically. The nanoscopic range first became accessible for fluorescence microscopy by single‐molecule techniques 17–19. Although single molecules are not resolved by these techniques, rather imaged as diffraction‐limited spots of about 220 nm diameter, their positions can be determined by curve fitting with high accuracy 20.…”

Tracking and Analyzing the Brownian Motion of Nano-objects Inside Hollow Core Fibers

“…Although the structures of viruses or proteins cannot be directly imaged with regular microscopy, their positions can be dynamically determined with high accuracy by fluorescent localization microscopy. − Fluorescence imaging has good spatial resolution but also has limitations, such as the demand for (invasive) labeling, finite photon budget, and bleaching, which all can be unfavorable regarding applications. − …”

Fluorophore Selection for Single‐Molecule Fluorescence Spectroscopy (SMFS) and Photobleaching Pathways

“…Thus, single-molecule fluorescence spectroscopy (SMFS) is used in a range of scientific areas in physics, chemistry, and biology, and has been explained in detail in various excellent reviews [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16].The ongoing success of SMFS has been facilitated not only through the development of optical single-molecule techniques, but in addition through new refined organic synthesis methods and the large repertoire of molecular biology techniques. From such an observation, you cannot answer questions about the individual routes that the trains took, how many passengers boarded the train at which station, and at what times, or how many stops, each train made.…”

Nanoscopic Medicine: The Next Frontier

“…16 Both techniques provide a lateral resolution of ≈220 nm and a vertical resolution of ≈600 nm, thus allowing thick samples to be sectioned optically. The nanoscopic range first became accessible for fluorescence microscopy by single‐molecule techniques 17–19. Although single molecules are not resolved by these techniques, rather imaged as diffraction‐limited spots of about 220 nm diameter, their positions can be determined by curve fitting with high accuracy 20.…”

Tracking and Analyzing the Brownian Motion of Nano-objects Inside Hollow Core Fibers

“…Although the structures of viruses or proteins cannot be directly imaged with regular microscopy, their positions can be dynamically determined with high accuracy by fluorescent localization microscopy. − Fluorescence imaging has good spatial resolution but also has limitations, such as the demand for (invasive) labeling, finite photon budget, and bleaching, which all can be unfavorable regarding applications. − …”

Fluorophore Selection for Single‐Molecule Fluorescence Spectroscopy (SMFS) and Photobleaching Pathways

“…Thus, single-molecule fluorescence spectroscopy (SMFS) is used in a range of scientific areas in physics, chemistry, and biology, and has been explained in detail in various excellent reviews [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16].The ongoing success of SMFS has been facilitated not only through the development of optical single-molecule techniques, but in addition through new refined organic synthesis methods and the large repertoire of molecular biology techniques. From such an observation, you cannot answer questions about the individual routes that the trains took, how many passengers boarded the train at which station, and at what times, or how many stops, each train made.…”

Nanoscopic Medicine: The Next Frontier

“…16 Both techniques provide a lateral resolution of ≈220 nm and a vertical resolution of ≈600 nm, thus allowing thick samples to be sectioned optically. The nanoscopic range first became accessible for fluorescence microscopy by single‐molecule techniques 17–19. Although single molecules are not resolved by these techniques, rather imaged as diffraction‐limited spots of about 220 nm diameter, their positions can be determined by curve fitting with high accuracy 20.…”