Single-Molecule Fluorescence Study on Membrane Proteins Derived from Living Organisms: Application to Drosophila Olfactory Receptor Or83b

Lee, Hong-Won; Lee, Han-Ki; Lee, Sion; Kim, Kipom; Min, Duyoung; Jones, Walton D.; Yoon, Taeyoung

doi:10.1016/j.bpj.2010.12.1048

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“…对细胞内信号转导通路的依赖也是细胞内钙离子浓度分析应用于检测嗅觉受体功能的一个缺点. [17,18] . 在哺乳动物中, 4 个不同蛋白家族, 包括非嗅觉受体类 G 蛋白偶联受体、受体转运蛋白(receptor transporting protein, RTP) 、受体表达增强蛋白 (receptor expression enhancing protein, REEP)和 70 kD 热休克蛋白(heat shock protein 70 kD, HSP70), 其中某些成员能促进嗅觉受体在 HEK-293 细胞中的表达、细胞膜定位和保持功能活性.…”

Section: × H I S 标签的适配体分子 ( 5 ′ -G C T a T G G G T G G -Tctggtunclassified

Functional expression of olfactory receptor and its application for biomimetic sensors

LiPing

ChunSheng

et al. 2013

Chin. Sci. Bull.

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ODR-10, an olfactory receptor protein of C. elegans, was used as a model of olfactory receptors expressed in human embryonic kidney (HEK)-293 cells as a fusing protein with 6×his-tag and rho-tag. The expression of the target protein was analyzed by RT-PCR (reverse transcription-polymerase chain reaction), Western blot and immunocytochemistry methods. The specific binding to odorant molecules of the olfactory receptor was measured using intracellular Ca 2+ assays. Then the olfactory receptor ODR-10 was immobilized onto the surface of quartz crystal microbalance (QCM) sensor to fabricate the olfactory receptor-based gas biosensor. The results demonstrated that this biosensor can detect the natural ligand of ODR-10 (diacetyl) with high sensitivity and specificity. All these results indicated that the olfactory receptor ODR-10 has been expressed successfully in the heterologous cell system and it can be used as the sensitive material in the development of biomimetic olfactory receptor-based biosensors.

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Section: × H I S 标签的适配体分子 ( 5 ′ -G C T a T G G G T G G -Tctggtunclassified

Functional expression of olfactory receptor and its application for biomimetic sensors

LiPing

ChunSheng

et al. 2013

Chin. Sci. Bull.

View full text Add to dashboard Cite

show abstract

Single-Molecule Fluorescence Study on Membrane Proteins Derived from Living Organisms: Application to Drosophila Olfactory Receptor Or83b

Cited by 1 publication

References 0 publications

Functional expression of olfactory receptor and its application for biomimetic sensors

Functional expression of olfactory receptor and its application for biomimetic sensors

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