Single living cell manipulation and identification using microsystems technologies

Stiharu, Ion; Alazzam, Anas; Nerguizian, Vahé; Roman, D.

doi:10.1038/micronano.2015.31

Cited by 21 publications

(14 citation statements)

References 34 publications

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“…AC‐based DEP requires nonuniform electric field which, in general, is created using conductive electrodes . The DEP force on a spherical polarizable microentity under nonuniform electric field while immersed in a conductive medium is defined by :

F_{DEP} = 2 π ε_{normalm} r^{3} Re [f_{cm}] \nabla (E_{rms}^{2})

where ε m is the permittivity of the medium, r is the radius of the microentity, E is the electric field, and Re[ f CM ] is the real part of Clausius–Mossotti factor that depends on the complex permittivities of the microentity and the suspended medium and is defined as:

f_{cm} = \frac{ε_{p}^{*} - ε_{m}^{*}}{ε_{p}^{*} + 2 ε_{m}^{*}}

where ε * is the complex permittivity that is a function of the dielectric constant ε , the angular frequency ω , and the real conductivity σ and is given by:

ε^{*} = ε - j \frac{σ}{ω}

where j is the imaginary unit,

j = \sqrt{- 1}

…”

Section: Methodsmentioning

confidence: 99%

“…AC-based DEP requires nonuniform electric field which, in general, is created using conductive electrodes [31,32]. The DEP force on a spherical polarizable microentity under nonuniform electric field while immersed in a conductive medium is defined by [25]:…”

Section: Theoretical Background and Separation Approachmentioning

confidence: 99%

“…The separation of cancer cells from among normal blood cells is performed at different ratios of cancer to normal blood cells. The number of CTCs in the blood of cancer patient is in order of 1-100 CTCs per 1 mL of whole blood [34,35]. Therefore, this work investigates the separation of cancer cells from among blood cells at low ratios of MDA-MB-231 cancer cells to normal blood cells; the ratios chosen are 1:10 3 , 1:10 4 , and 1:10 5 .…”

Section: Suspension Medium and Cellsmentioning

confidence: 99%

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Novel microfluidic device for the continuous separation of cancer cells using dielectrophoresis

2017

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We describe the design, microfabrication, and testing of a microfluidic device for the separation of cancer cells based on dielectrophoresis. Cancer cells, specifically green fluorescent protein-labeled MDA-MB-231, are successfully separated from a heterogeneous mixture of the same and normal blood cells. MDA-MB-231 cancer cells are separated with an accuracy that enables precise detection and counting of circulating tumor cells present among normal blood cells. The separation is performed using a set of planar interdigitated transducer electrodes that are deposited on the surface of a glass wafer and slightly protrude into the separation microchannel at one side. The device includes two parts, namely, a glass wafer and polydimethylsiloxane element. The device is fabricated using standard microfabrication techniques. All experiments are conducted with low conductivity sucrose-dextrose isotonic medium. The variation in response between MDA-MB-231 cancer cells and normal cells to a certain band of alternating-current frequencies is used for continuous separation of cells. The fabrication of the microfluidic device, preparation of cells and medium, and flow conditions are detailed. The proposed microdevice can be used to detect and separate malignant cells from heterogeneous mixture of cells for the purpose of early screening for cancer.

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F_{DEP} = 2 π ε_{normalm} r^{3} Re [f_{cm}] \nabla (E_{rms}^{2})

f_{cm} = \frac{ε_{p}^{*} - ε_{m}^{*}}{ε_{p}^{*} + 2 ε_{m}^{*}}

where ε * is the complex permittivity that is a function of the dielectric constant ε , the angular frequency ω , and the real conductivity σ and is given by:

ε^{*} = ε - j \frac{σ}{ω}

where j is the imaginary unit,

j = \sqrt{- 1}

…”

Section: Methodsmentioning

confidence: 99%

Section: Theoretical Background and Separation Approachmentioning

confidence: 99%

Section: Suspension Medium and Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Novel microfluidic device for the continuous separation of cancer cells using dielectrophoresis

2017

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“…The generated inhomogeneous electric field induces lateral motion on cells because of dielectrophoresis (DEP). The DEP is defined as the motion of neutral and polarizable particles or cells suspended in a conductive medium under the effect of inhomogeneous electric field [36][37][38]. It is a label-free manipulation method with low impact on the cellular integrity [38].…”

Section: Living Cells Manipulationmentioning

confidence: 99%

Micro-Pattern of Graphene Oxide Films Using Metal Bonding

et al. 2020

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Recently, graphene has been explored in several research areas according to its outstanding combination of mechanical and electrical features. The ability to fabricate micro-patterns of graphene facilitates its integration in emerging technologies such as flexible electronics. This work reports a novel micro-pattern approach of graphene oxide (GO) film on a polymer substrate using metal bonding. It is shown that adding ethanol to the GO aqueous dispersion enhances substantially the uniformity of GO thin film deposition, which is a great asset for mass production. On the other hand, the presence of ethanol in the GO solution hinders the fabrication of patterned GO films using the standard lift-off process. To overcome this, the fabrication process provided in this work takes advantage of the chemical adhesion between the GO or reduced GO (rGO) and metal films. It is proved that the adhesion between the metal layer and GO or rGO is stronger than the adhesion between the latter and the polymer substrate (i.e., cyclic olefin copolymer used in this work). This causes the removal of the GO layer underneath the metal film during the lift-off process, leaving behind the desired GO or rGO micro-patterns. The feasibility and suitability of the proposed pattern technique is confirmed by fabricating the patterned electrodes inside a microfluidic device to manipulate living cells using dielectrophoresis. This work adds great value to micro-pattern GO and rGO thin films and has immense potential to achieve high yield production in emerging applications.

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“…Although much research has been conducted about the use of DEP for biological and medical purposes, such as the isolation of rare circulating tumour cells at concentrations lower than one in a million , there have been relatively few studies on its potential uses for IVF. There is however, great potential for its application, as it is an extremely effective sorting technique for rare cells.…”

Section: Introductionmentioning

confidence: 99%

Distinct and independent dielectrophoretic behavior of the head and tail of sperm and its potential for the safe sorting and isolation of rare spermatozoa

Shuchat

Park

Kol³

et al. 2019

Electrophoresis

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Often, in semen samples with minute amounts of sperm, even the single spermatozoon required to fertilize an oocyte cannot be found in the ejaculate. This is primarily because currently, sperm is generally searched for manually under a microscope. In this study, dielectrophoresis (DEP) was investigated as an alternative automated technique for sorting sperm cells. Using a quadrupolar electrode array it was shown that the head and tail of the sperm had independent and unique crossover frequencies corresponding to the transition of the DEP force from repulsive (negative) to attractive (positive). These surprising results were further analyzed, showing that the head and tail have their own distinct electrical properties. This significant result allows for the sperm's head, which contains the DNA, to be distanced from potentially damaging high electric fields using negative DEP while simultaneously manipulating and sorting the sperm using the positive DEP response of the tail. A proof of concept sorting chip was designed and tested. The low crossover frequency of the tail also allows for the use of a higher conductivity, and thus more physiological, medium than the conventional DEP solutions. Although more research is required to design and optimize an efficient, user‐friendly, and high‐throughput device, this research is a proof of concept that DEP has the potential to automate and improve the processing of semen samples, especially those containing only rare spermatozoa.

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Single living cell manipulation and identification using microsystems technologies

Cited by 21 publications

References 34 publications

Novel microfluidic device for the continuous separation of cancer cells using dielectrophoresis

Novel microfluidic device for the continuous separation of cancer cells using dielectrophoresis

Micro-Pattern of Graphene Oxide Films Using Metal Bonding

Distinct and independent dielectrophoretic behavior of the head and tail of sperm and its potential for the safe sorting and isolation of rare spermatozoa

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