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Cited by 61 publications
(63 citation statements)
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References 11 publications
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“…Although the recovery rate is small compared with those obtained in stallions (Morrell et al, 2009c(Morrell et al, , 2011a, these differences can be explained by this colloid formulation, which has been previously used with stallions and not with donkeys. This fact can be explained attending to previous studies performed with horses , where it is described that volume and concentration must be adjusted to a specific colloid.…”
Section: Discussionmentioning
confidence: 75%
See 1 more Smart Citation
“…Although the recovery rate is small compared with those obtained in stallions (Morrell et al, 2009c(Morrell et al, , 2011a, these differences can be explained by this colloid formulation, which has been previously used with stallions and not with donkeys. This fact can be explained attending to previous studies performed with horses , where it is described that volume and concentration must be adjusted to a specific colloid.…”
Section: Discussionmentioning
confidence: 75%
“…The formulation for stallions is Androcoll-E (Johannisson et al, 2009) and has been recently commercialized by Minitüb GmbH (Tiefenbach, Germany). Androcoll-E TM has been used to select robust spermatozoa in terms of motile and morphologically normal sperm, with intact membranes and good chromatin integrity (Morrell et al, 2009c). On the basis of all this sperm parameters to perform the sperm quality analysis, Androcoll-E has improved the quality of fresh (Morrell et al, 2009a), frozen (Macias Garcia et al, 2009a;Macias Garcia et al, 2009b;Hoogewijs et al, 2011) and cooled-stored (Morrell et al, 2009d;Bergqvist et al, 2011) stallion sperm samples with a shorter preparation time and less complicated process than the conventional density gradient centrifugation .…”
Section: Introductionmentioning
confidence: 99%
“…Edmond et al (2012) drew attention to a downside for the use of colloidal centrifugation in stallions related to the characteristic large volume of stallion ejaculates, as compared to that of humans and most domestic animals. Morrell et al (2009b) addressed this issue by increasing the size of the centrifuge tubes in order to accommodate a larger volume of semen per tube and, consequently, reducing the number of tubes needed for colloidal centrifugation of stallion ejaculates. However, as suggested by Edmond et al (2012), this "scaling-up" imposes an economic constraint for most breeding studs, which are typically not equipped with the centrifugal rotors necessary for large tubes, and also because a large volume of the colloidal product is required per tube and to process the whole stallion ejaculate.…”
Section: Concmentioning
confidence: 99%
“…This formulation incorporated silane-coated silica in an optimised salt solution instead of Percoll ™ (polyvinylpyrrolidonecoated silica) to which various salt solutions had been added. The original SLC methodology was later modified to enable larger volumes of ejaculate to be processed without compromising sperm quality in the resulting preparations [6], a feature that was not possible with DGC without a drop in sperm yield [7]. The initial stage of preparing a scaledup SLC with Androcoll-E and stallion semen is shown in (Figure 1).…”
Section: Introductionmentioning
confidence: 99%
“…The initial stage of preparing a scaledup SLC with Androcoll-E and stallion semen is shown in (Figure 1). It was possible to prepare an average gel-free stallion ejaculate using six to eight 50-mL centrifuge tubes using the SLC-Large method [6].…”
Section: Introductionmentioning
confidence: 99%