Single-Gene Greenbeard Effects in the Social Amoeba
            <i>Dictyostelium discoideum</i>

Queller, David C.; Ponte, E; Bozzaro, Salvatore; Strassmann, Joan E.

doi:10.1126/science.1077742

Cited by 280 publications

(244 citation statements)

References 17 publications

(6 reference statements)

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“…Variation in adhesion can have an impact on cell fate, because the cells at the front of the slug become stalk and the cells in the back threequarters or so become spore (62). One way of increasing the likelihood of becoming spore could therefore be to have reduced adhesion to the other cells and to slip back in the slug (63,64). The knockout of the cell adhesion gene csaA has just this effect.…”

Section: Control Of Cheatingmentioning

confidence: 99%

“…When csaA is knocked out, adhesion is reduced. On agar, this has the impact of increasing the knockout's frequency in the spores, presumably because reduced adhesion allows it to slip out of the stalk-forming tip (63,64). On the more natural substrate of soil, however, csaA knockouts apparently do not hold together enough to get into aggregations.…”

Section: Control Of Cheatingmentioning

confidence: 99%

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Evolution of cooperation and control of cheating in a social microbe

Strassmann

Queller

2011

Proc. Natl. Acad. Sci. U.S.A.

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Much of what we know about the evolution of altruism comes from animals. Here, we show that studying a microbe has yielded unique insights, particularly in understanding how social cheaters are controlled. The social stage of Dictylostelium discoideum occurs when the amoebae run out of their bacterial prey and aggregate into a multicellular, motile slug. This slug forms a fruiting body in which about a fifth of cells die to form a stalk that supports the remaining cells as they form hardy dispersal-ready spores. Because this social stage forms from aggregation, it is analogous to a social group, or a chimeric multicellular organism, and is vulnerable to internal conflict. Advances in cell labeling, microscopy, single-gene knockouts, and genomics, as well as the results of decades of study of D. discoideum as a model for development, allow us to explore the genetic basis of social contests and control of cheaters in unprecedented detail. Cheaters are limited from exploiting other clones by high relatedness, kin discrimination, pleiotropy, noble resistance, and lottery-like role assignment. The active nature of these limits is reflected in the elevated rates of change in social genes compared with nonsocial genes. Despite control of cheaters, some conflict is still expressed in chimeras, with slower movement of slugs, slightly decreased investment in stalk compared with spore cells, and differential contributions to stalk and spores. D. discoideum is rapidly becoming a model system of choice for molecular studies of social evolution.kin selection | kin recognition | multicellularity | major transitions

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Section: Control Of Cheatingmentioning

confidence: 99%

Section: Control Of Cheatingmentioning

confidence: 99%

Evolution of cooperation and control of cheating in a social microbe

Strassmann

Queller

2011

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

190

200

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“…However, disruption of the cadA gene leads to aberrant cell sorting and defects in cell-type proportioning (Wong et al, 2002), suggesting a role for DdCAD-1 in the post-aggregation phase of development. Changes in the environmental conditions of development have been used successfully to investigate subtle functions of cell adhesion proteins (Ponte et al, 1998;Queller et al, 2003), we have taken a similar approach to investigate the role of DdCAD-1 in the post-aggregation stages of development. Chimeras of wild-type and mutant cells were analyzed under different environmental conditions.…”

Section: Resultsmentioning

confidence: 99%

“…This phenomenon raises the issues of how social cooperation persists in nature and what factors would permit individuals with a compromised phenotype to survive (Foster et al, 2004;Nowak, 2006;Gilbert et al, 2007). Among the many potential factors, cells tend to cooperate with genetically similar individuals and the cell adhesion genes csaA and tgrC1 have been found to confer survival advantages to D. discoideum cells that carry the same or highly related genes (Queller et al, 2003;Benabentos et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

The cell adhesion molecule DdCAD-1 regulates morphogenesis through differential spatiotemporal expression inDictyostelium discoideum

et al. 2011

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SUMMARYDuring development of Dictyostelium, multiple cell types are formed and undergo a coordinated series of morphogenetic movements guided by their adhesive properties and other cellular factors. DdCAD-1 is a unique homophilic cell adhesion molecule encoded by the cadA gene. It is synthesized in the cytoplasm and transported to the plasma membrane by contractile vacuoles. In chimeras developed on soil plates, DdCAD-1-expressing cells showed greater propensity to develop into spores than did cadA-null cells. When development was performed on non-nutrient agar, wild-type cells sorted from the cadA-null cells and moved to the anterior zone. They differentiated mostly into stalk cells and eventually died, whereas the cadA-null cells survived as spores. To assess the role of DdCAD-1 in this novel behavior of wild-type and mutant cells, cadA-null cells were rescued by the ectopic expression of DdCAD-1-GFP. Morphological studies have revealed major spatiotemporal changes in the subcellular distribution of DdCAD-1 during development. Whereas DdCAD-1 became internalized in most cells in the post-aggregation stages, it was prominent in the contact regions of anterior cells. Cell sorting was also restored in cadA -slugs by exogenous recombinant DdCAD-1. Remarkably, DdCAD-1 remained on the surface of anterior cells, whereas it was internalized in the posterior cells. Additionally, DdCAD-1-expressing cells migrated slower than cadA -cells and sorted to the anterior region of chimeric slugs. These results show that DdCAD-1 influences the sorting behavior of cells in slugs by its differential distribution on the prestalk and prespore cells.

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“…Higashiyama, 2010;Rea and Nasrallah, 2008), and in cooperation between sperm in deer mice, where sperm cells of the same male tend to cluster to a higher extent than mixtures of sperm cells from related and unrelated males (Fisher and Hoekstra, 2010). Self/nonself recognition also seems to play a role in aggregation formation in the budding yeast Saccharomyces cerevisiae (Smukalla et al, 2008) and the social amoeba Dictyostelium discoideum (Queller et al, 2003). In both species, proteins encoded by a single gene are responsible for the clustering of individuals carrying that same gene.…”

Section: Proteins Are Likely To Play Key Roles In the (In) Capacitatimentioning

confidence: 99%