Single DNA Molecule Isolation and Trapping in a Microfluidic Device

Kumemura, Momoko; Collard, Dominique; Yamahata, Christophe; Sakaki, N.; Hashiguchi, Gen; Fujita, Hiroyuki

doi:10.1002/cphc.200700268

Cited by 42 publications

(45 citation statements)

References 29 publications

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“…Electrophoretic and DEP forces were combined to improve separation by Kumemura et al and Regtmeier et al [119,125]. In the method by Kumemura et al, individual double-stranded DNA migrated through the device via electrophoretic force but was captured using DEP [119].…”

Section: Analysis Of Dnamentioning

confidence: 98%

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Bioanalytical separations using electric field gradient techniques

2009

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The field of separations science will be strongly impacted by new electric-field-gradient-based strategies. Many new capabilities are being developed with analytical targets ranging from particles to small molecules, and soot to living cells. Here we review the emerging area of electric field gradient techniques, dividing the large variety of techniques by the target of separation. In doing so, we have contributions using dielectrophoresis, electric field gradient focusing (including dynamic, true moving bed, and pulsed field), electrocapture and electrophoretic focusing, temperature gradient focusing, and focusing with centrifugal force. We cover the literature from the start of 2007 to June 2008, along with some introductory discussions. Even with the relatively short time frame, this young and dynamic field of inquiry produced some 100 contributions describing new and unique techniques and several new applications.

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Section: Analysis Of Dnamentioning

confidence: 98%

“…In the method by Kumemura et al, individual double-stranded DNA migrated through the device via electrophoretic force but was captured using DEP [119]. The device captured a single, long strand (10 mm) 48.5 kbp l-DNA.…”

Section: Analysis Of Dnamentioning

confidence: 99%

Bioanalytical separations using electric field gradient techniques

2009

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“…Cohen et al developed an anti-Brownian electrokinetic trap [56], in which a time-dependent electric field was applied to induce the electrokinetic drift to cancel the random diffusion of single molecules in aqueous solution. Kumemura et al reported another trapping device using AC dielectrophoresis [57], in which individual DNA molecules were extended and oriented in the electric field and anchored between the aluminum electrodes for long-term studies. The fluid fieldinduced single-molecule trapping at the intersection of microfluidic channels was reported recently [58].…”

Section: Smd In Integrated Microfluidic Devicesmentioning

confidence: 99%

Recent advances in single‐molecule detection on micro‐ and nano‐fluidic devices

Liu¹,

Qu²,

Luo³

et al. 2011

Electrophoresis

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Single-molecule detection (SMD) allows static and dynamic heterogeneities from seemingly equal molecules to be revealed in the studies of molecular structures and intra- and inter-molecular interactions. Micro- and nanometer-sized structures, including channels, chambers, droplets, etc., in microfluidic and nanofluidic devices allow diffusion-controlled reactions to be accelerated and provide high signal-to-noise ratio for optical signals. These two active research frontiers have been combined to provide unprecedented capabilities for chemical and biological studies. This review summarizes the advances of SMD performed on microfluidic and nanofluidic devices published in the past five years. The latest developments on optical SMD methods, microfluidic SMD platforms, and on-chip SMD applications are discussed herein and future development directions are also envisioned.

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“…Over the past several decades, the separation and concentration of biomolecules including DNA and proteins have been developed for micro-scale assay such as genetics, disease diagnostics and point-of-care applications [1]. Recently, novel biomolecules separation and detection methods based on ion concentration polarization (ICP) phenomena have been extensively developed [2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…All authors read and approved the final manuscript. 1 Department of Electrical and Computer Engineering, Seoul National University, Seoul 08826, Republic of Korea.…”

mentioning

confidence: 99%

Paper-based ion concentration polarization device for selective preconcentration of muc1 and lamp-2 genes

Son

Lee

Kim

2017

Micro and Nano Syst Lett

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Recently, novel biomolecules separation and detection methods based on ion concentration polarization (ICP) phenomena have been extensively researched due to its high amplification ratio and high-speed accumulation. Despite of these bright advances, the fabrication of conventional ICP devices still have complicated and times-consuming tasks. As an alternative platform, a paper have been recently used for the identical ICP operations. In this work, we demonstrated the selective preconcentration of a muc1 gene fragment as human breast cancer marker and a lamp-2 gene fragment as the cause of Danon disease in paper-based ICP devices. As a result, these two DNA fragments were successfully concentrated up to ~60 fold at different location in a single paper-channel. The device would be a promising platform for point-of-care device due to an economic fabrication, the easy extraction of concentrated sample and an easy disposability.

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Single DNA Molecule Isolation and Trapping in a Microfluidic Device

Cited by 42 publications

References 29 publications

Bioanalytical separations using electric field gradient techniques

Bioanalytical separations using electric field gradient techniques

Recent advances in single‐molecule detection on micro‐ and nano‐fluidic devices

Paper-based ion concentration polarization device for selective preconcentration of muc1 and lamp-2 genes

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