Single‐cell transcriptome atlas identified novel regulators for pigment gland morphogenesis in cotton

Long, Lu; Xu, Fuchun; Chun-hu, Wang; Zhao, Xiao-Tong; Yuan, Man; Song, Chun‐Peng; Gao, Wei

doi:10.1111/pbi.14035

Cited by 17 publications

(19 citation statements)

References 4 publications

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“…S1). In a previous study, we used single‐cell sequencing to show that GoPGF is specifically expressed in the pigment gland cells of cotyledons (Long et al ., 2023). To establish whether GoPGF expression is specifically localized to pigment gland cells in other tissues, we subjected the T 2 generation of the ProGoPGF‐GUS‐expressing transgenic cotton to GUS staining.…”

Section: Resultsmentioning

confidence: 99%

“…In a previous study, the gland‐specific GhJUB1 was identified through a single‐cell sequencing of cotton cotyledon, and the GhJUB1 was proposed to be located downstream of GoPGF to regulate gland morphogenesis (Long et al ., 2023). In this study, GhJUB1 was identified as the direct target of GoPGF through ChIP‐seq (Table S2).…”

Section: Resultsmentioning

confidence: 99%

“…The study found that gossypol produced in WT roots failed to replenish the gossypol content of the ultra-low gossypol cottonseed, implying that the gossypol accumulation in the aboveground parts is unrelated to the roots. Furthermore, through single-cell transcriptome analysis using protoplasts isolated from cotton cotyledon, Long et al (2023) and Sun et al (2023) revealed that several gossypol synthetic genes are highly expressed in the pigment gland cells but not in the other cells. Our study demonstrated that gossypol biosynthetic genes in gland cells exhibited expression levels hundreds to thousands of times higher than in NGCs within the same leaf.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, a NAC transcription factor, GhJUB1 , was found to be specifically expressed in pigment gland cells through single‐cell sequencing. Subsequent silencing of GhJUB1 resulted in a substantial reduction of pigment glands in cotton (Long et al ., 2023). The genes regulating pigment gland appearance were also reported to affect gossypol accumulation because the lack of the gland cavity is usually coupled with a dramatic reduction of gossypol (Ma et al ., 2016; Janga et al ., 2019; Li et al ., 2021; Zang et al ., 2021; Long et al ., 2023; Sun et al ., 2023).…”

Section: Introductionmentioning

confidence: 99%

“…Subsequent silencing of GhJUB1 resulted in a substantial reduction of pigment glands in cotton (Long et al ., 2023). The genes regulating pigment gland appearance were also reported to affect gossypol accumulation because the lack of the gland cavity is usually coupled with a dramatic reduction of gossypol (Ma et al ., 2016; Janga et al ., 2019; Li et al ., 2021; Zang et al ., 2021; Long et al ., 2023; Sun et al ., 2023). In cotton breeding, the presence, size and density of pigment glands are often used as indicators to measure gossypol content.…”

Section: Introductionmentioning

confidence: 99%

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The dual role of GoPGF reveals that the pigment glands are synthetic sites of gossypol in aerial parts of cotton

Zhang,

Long,

Zhao

et al. 2023

New Phytologist

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Summary Gossypol and the related terpenoids are stored in the pigment gland to protect cotton plants from biotic stresses, but little is known about the synthetic sites of these metabolites. Here, we showed that GoPGF, a key gene regulating gland formation, was expressed in gland cells and roots. The chromatin immunoprecipitation sequencing (ChIP‐seq) analysis demonstrated that GoPGF targets GhJUB1 to regulate gland morphogenesis. RNA‐sequencing (RNA‐seq) showed high accumulation of gossypol biosynthetic genes in gland cells. Moreover, integrated analysis of the ChIP‐seq and RNA‐seq data revealed that GoPGF binds to the promoter of several gossypol biosynthetic genes. The cotton callus overexpressing GoPGF had dramatically increased the gossypol levels, indicating that GoPGF can directly activate the biosynthesis of gossypol. In addition, the gopgf mutant analysis revealed the existence of both GoPGF‐dependent and ‐independent regulation of gossypol production in cotton roots. Our study revealed that the pigment glands are synthetic sites of gossypol in aerial parts of cotton and that GoPGF plays a dual role in regulating gland morphogenesis and gossypol biosynthesis. The study provides new insights for exploring the complex relationship between glands and the metabolites they store in cotton and other plant species.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The dual role of GoPGF reveals that the pigment glands are synthetic sites of gossypol in aerial parts of cotton

Zhang,

Long,

Zhao

et al. 2023

New Phytologist

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Regulation of Glandular Size and Phytoalexin Biosynthesis by a Negative Feedback Loop in Cotton

Wu,

Nie,

Lin

et al. 2024

Advanced Science

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Plants have evolved diverse defense mechanisms encompassing physical and chemical barriers. Cotton pigment glands are known for containing various defense metabolites, but the precise regulation of gland size to modulate defense compound levels remains enigmatic. Here, it is discovered that the VQ domain‐containing protein JAVL negatively regulates pigment gland size and the biosynthesis of defense compounds, while the MYC2‐like transcription factor GoPGF has the opposite effect. Notably, GoPGF directly activates the expression of JAVL, whereas JAVL suppresses GoPGF transcription, establishing a negative feedback loop that maintains the expression homeostasis between GoPGF and JAVL. Furthermore, it is observed that JAVL negatively regulates jasmonate levels by inhibiting the expression of jasmonate biosynthetic genes and interacting with GoPGF to attenuate its activation effects, thereby maintaining homeostatic regulation of jasmonate levels. The increased expression ratio of GoPGF to JAVL leads to enlarged pigment glands and elevated jasmonates and defense compounds, enhancing insect and pathogen resistance in cotton. These findings unveil a new mechanism for regulating gland size and secondary metabolites biosynthesis, providing innovative strategies for strengthening plant defense.

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Genomic study of PIFs in cotton and functional analysis of GhPIF4 in promoting cotton flowering

Zhao,

Feng,

Zheng

et al. 2023

Plant Growth Regul

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Single‐cell transcriptome atlas identified novel regulators for pigment gland morphogenesis in cotton

Cited by 17 publications

References 4 publications

The dual role of GoPGF reveals that the pigment glands are synthetic sites of gossypol in aerial parts of cotton

The dual role of GoPGF reveals that the pigment glands are synthetic sites of gossypol in aerial parts of cotton

Regulation of Glandular Size and Phytoalexin Biosynthesis by a Negative Feedback Loop in Cotton

Genomic study of PIFs in cotton and functional analysis of GhPIF4 in promoting cotton flowering

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