Single cell sequencing analysis of lizard phagocytic cell populations and their role in tail regeneration

Londoño, Ricardo; Tighe, Sean; Milnes, Beatrice; DeMoya, Christian; Quijano, Lina M.; Hudnall, Megan L.; Nguyen, Joseph; Tran, Elizabeth; Badylak, Stephen F.; Lozito, Thomas P.

doi:10.1016/j.regen.2020.100029

Cited by 17 publications

(31 citation statements)

References 24 publications

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“…Another technology, single cell RNA sequencing, has emerged and allows for unprecedented resolution of molecular activities at the level of individual cells. This approach is particularly useful for studying in vivo contexts of developmental mechanisms and has been utilized for investigating cell fate during embryogenesis (Blase, Cao, & Zhong, 2014; Chu et al, 2016; Farrell et al, 2018; Li et al, 2019; Tang et al, 2010; Wagner et al, 2018; Yan et al, 2013) and regeneration (Aztekin et al, 2019; Ba, Wang, Wu, Sun, & Li, 2019; Carr et al, 2019; Johnson, Masias, & Lehoczky, 2020; Leigh et al, 2018; Londono et al, 2020) in various models. Importantly, it will prove invaluable for investigating the contribution of specific cell populations and lineages in the development of OFCs.…”

Section: Discussionmentioning

confidence: 99%

Cellular and developmental basis of orofacial clefts

Garland

Sun

et al. 2020

Birth Defects Research

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During craniofacial development, defective growth and fusion of the upper lip and/or palate can cause orofacial clefts (OFCs), which are among the most common structural birth defects in humans. The developmental basis of OFCs includes morphogenesis of the upper lip, primary palate, secondary palate, and other orofacial structures, each consisting of diverse cell types originating from all three germ layers: the ectoderm, mesoderm, and endoderm. Cranial neural crest cells and orofacial epithelial cells are two major cell types that interact with various cell lineages and play key roles in orofacial development. The cellular basis of OFCs involves defective execution in any one or several of the following processes: neural crest induction, epithelial‐mesenchymal transition, migration, proliferation, differentiation, apoptosis, primary cilia formation and its signaling transduction, epithelial seam formation and disappearance, periderm formation and peeling, convergence and extrusion of palatal epithelial seam cells, cell adhesion, cytoskeleton dynamics, and extracellular matrix function. The latest cellular and developmental findings may provide a basis for better understanding of the underlying genetic, epigenetic, environmental, and molecular mechanisms of OFCs.

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Section: Discussionmentioning

confidence: 99%

Cellular and developmental basis of orofacial clefts

Garland

Sun

et al. 2020

Birth Defects Research

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“…A recent study explored the role of macrophages in lizard regenerating tail, revealing an increase in the phagocytic cell population within the blastema starting from 3 days post-amputation. Clodronate liposome treated lizards failed to form blastema and to regrow amputated tail, further confirming the importance of macrophages in regeneration [ 144 ]. Another recent work reported the presence of anti-inflammatory M2-like macrophages underneath the wound epidermis and near the ependyma in the regenerating Podarcis muralis tail, which similarly to the amphibian limb, support and stimulate regeneration [ 145 ].…”

Section: Role Of Inflammationmentioning

confidence: 86%

“…Taken together, these results indicate that a timely activation of macrophages is required during the early steps of regeneration for a correct blastema formation and the onset of the regenerative process. During this phase, macrophages contribute to the establishment of an inflammatory environment through the secretion of cytokines and MMPs to degrade ECM components and tissue debris facilitating cell migration and communication [ 17 , 143 , 144 ]. Furthermore, the presence of an early anti-inflammatory response is related to a resolutive and anti-fibrotic activity that may be critical to both resolve inflammation and to carry on regeneration [ 147 ] by preventing fibrotic deposition and scarring [ 142 ].…”

Section: Role Of Inflammationmentioning

confidence: 99%

Appendage Regeneration in Vertebrates: What Makes This Possible?

Daponte

Tylżanowski

Forlino

2021

Cells

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The ability to regenerate amputated or injured tissues and organs is a fascinating property shared by several invertebrates and, interestingly, some vertebrates. The mechanism of evolutionary loss of regeneration in mammals is not understood, yet from the biomedical and clinical point of view, it would be very beneficial to be able, at least partially, to restore that capability. The current availability of new experimental tools, facilitating the comparative study of models with high regenerative ability, provides a powerful instrument to unveil what is needed for a successful regeneration. The present review provides an updated overview of multiple aspects of appendage regeneration in three vertebrates: lizard, salamander, and zebrafish. The deep investigation of this process points to common mechanisms, including the relevance of Wnt/β-catenin and FGF signaling for the restoration of a functional appendage. We discuss the formation and cellular origin of the blastema and the identification of epigenetic and cellular changes and molecular pathways shared by vertebrates capable of regeneration. Understanding the similarities, being aware of the differences of the processes, during lizard, salamander, and zebrafish regeneration can provide a useful guide for supporting effective regenerative strategies in mammals.

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“…reactive oxygen species [101][102][103][104], hydrogen pump activity [105] and oxygen influx [106]) can determine the regeneration outcome. Successful appendage regeneration, so far, has been shown to require the presence of macrophages (more broadly myeloid lineage) in a vast selection of regenerative vertebrates through their impact on the changes in ECM (zebrafish fin [107,108]; axolotl limb [109]; mouse digit tip [110,111]; lizard tail [112]; and Xenopus tail [113]). Additionally, amputations induce apoptosis as well as cellular senescence, and their clearance via immune cells was also suggested to facilitate the growth of the new appendage [114].…”

Section: Regeneration-permissive Environmentmentioning

confidence: 99%

Appendage regeneration is context dependent at the cellular level

Aztekin

2021

Open Biol.

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Species that can regrow their lost appendages have been studied with the ultimate aim of developing methods to enable human limb regeneration. These examinations highlight that appendage regeneration progresses through shared tissue stages and gene activities, leading to the assumption that appendage regeneration paradigms (e.g. tails and limbs) are the same or similar. However, recent research suggests these paradigms operate differently at the cellular level, despite sharing tissue descriptions and gene expressions. Here, collecting the findings from disparate studies, I argue appendage regeneration is context dependent at the cellular level; nonetheless, it requires (i) signalling centres, (ii) stem/progenitor cell types and (iii) a regeneration-permissive environment, and these three common cellular principles could be more suitable for cross-species/paradigm/age comparisons.

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Single cell sequencing analysis of lizard phagocytic cell populations and their role in tail regeneration

Cited by 17 publications

References 24 publications

Cellular and developmental basis of orofacial clefts

Cellular and developmental basis of orofacial clefts

Appendage Regeneration in Vertebrates: What Makes This Possible?

Appendage regeneration is context dependent at the cellular level

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