2018
DOI: 10.1038/s41467-018-06318-7
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Abstract: The liver is the largest solid organ in the body and is critical for metabolic and immune functions. However, little is known about the cells that make up the human liver and its immune microenvironment. Here we report a map of the cellular landscape of the human liver using single-cell RNA sequencing. We provide the transcriptional profiles of 8444 parenchymal and non-parenchymal cells obtained from the fractionation of fresh hepatic tissue from five human livers. Using gene expression patterns, flow cytometr… Show more

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Cited by 985 publications
(1,158 citation statements)
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References 86 publications
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“…The authors then shifted focus to immune cell populations and defined inflammatory and immunoregulatory subpopulations of Kupffer cells, in agreement with a previous study . In addition, they described the gene signatures of circulating and resident populations of B cells, defined by MS4A1 and CD37 expression and MZB1 , DERL3 , SSR4 , and IGHG4 expression, respectively.…”
supporting
confidence: 74%
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“…The authors then shifted focus to immune cell populations and defined inflammatory and immunoregulatory subpopulations of Kupffer cells, in agreement with a previous study . In addition, they described the gene signatures of circulating and resident populations of B cells, defined by MS4A1 and CD37 expression and MZB1 , DERL3 , SSR4 , and IGHG4 expression, respectively.…”
supporting
confidence: 74%
“…Overall, this study is the most comprehensive single‐cell gene profiling of the human liver to date, thus providing a powerful resource to the biomedical research community. Despite a broad experimental scope, Aizarani and colleagues were able to reproduce key findings from complementary studies, lending credence to their in silico approach to lineage reconstruction. However, ambiguity remains surrounding the definition of TROP2‐intermediate liver progenitor cells.…”
mentioning
confidence: 94%
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“…These studies provided an important first step in unraveling the molecular and functional heterogeneity of liver endothelial cells. Interestingly, liver macrophages also exhibited features of zonation, as illustrated by gradient expression of the scavenger receptor MARCO . Future work is needed to dissect the signaling mechanisms underlying liver cell zonation and its role in liver physiology and disease.…”
Section: Liver Cell Heterogeneity and Zonation At Single‐cell Resolutionmentioning
confidence: 99%
“…The whole‐liver single‐cell RNA‐Seq effectively identified genes that are characteristic for activated hHSCs, but the depth of sequencing failed to identify unique and disease‐specific genes. Single‐cell RNA analysis of human livers recently revealed novel markers of human HSCs . Although the single‐cell analysis identified genes that distinguish HSCs from other hepatic cell types, we identified some of the same genes in our study (including CTSS , CD74 , CYBA , ID1 , FSTL3 , and FMOD ).…”
Section: Discussionmentioning
confidence: 63%