Single Cell Array Impedance Analysis for Cell Detection and Classification in a Microfluidic Device

Altinagac, Emre; Taskin, Selen; Kızıl, Hüseyin

doi:10.5220/0006166700490053

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…As for the permeability testing, an LCR metre was chosen to measure the impedance. Impedance testing has been previously reported to be sensitive to the cell type that could be stimulated by different chemicals or drugs [ 36 ,[ 37 ]. Likewise, we hypothesized that the uptake of antibiotics into the GUVs could be monitored by a simple impedance testing because the outer membrane of GUVs in solution comprises an RC circuit [ 38 , 39 , 40 ] (Figure 4 ).…”

Section: Resultsmentioning

confidence: 99%

On‐chip label‐free impedance‐based detection of antibiotic permeation

Kaur

Ghorbanpoor

Öztürk

et al. 2021

IET Nanobiotechnology

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Biosensors are analytical tools used for the analysis of biomaterial samples and provide an understanding about the biocomposition, structure, and function of biomolecules and/or biomechanisms by converting the biological response into an electrical and/or optical signal. In particular, with the rise in antibiotic resistance amongst pathogenic bacteria, the study of antibiotic activity and transport across cell membranes in the field of biosensors has been gaining widespread importance. Herein, for the rapid and label-free detection of antibiotic permeation across a membrane, a microelectrode integrated microfluidic device is presented. The integrated chip consists of polydimethylsiloxane based microfluidic channels bonded onto microelectrodes on-glass and enables us to recognize the antibiotic permeation across a membrane into the model membranes based on electrical impedance measurement, while also allowing optical monitoring. Impedance testing is label free and therefore allows the detection of both fluorescent and non-fluorescent antibiotics. As a model membrane, Giant Unilamellar Vesicles (GUVs) are used and impedance measurements were performed by a precision inductance, capacitance, and resistance metre. The measured signal recorded from the device was used to determine the change in concentration inside and outside of the GUVs. We have found that permeation of antibiotic molecules can be easily monitored over time using the proposed integrated device. The results also show a clear difference between bilayer permeation that occurs through the lipidic bilayer and porin-mediated permeation through the porin channels inserted in the lipid bilayer. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Resultsmentioning

confidence: 99%

On‐chip label‐free impedance‐based detection of antibiotic permeation

Kaur

Ghorbanpoor

Öztürk

et al. 2021

IET Nanobiotechnology

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Bioelectrical Analysis of Various Cancer Cell Types Immobilized in 3D Matrix and Cultured in 3D-Printed Well

et al. 2019

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Cancer cell lines are important tools for anticancer drug research and assessment. Impedance measurements can provide valuable information about cell viability in real time. This work presents the proof-of-concept development of a bioelectrical, impedance-based analysis technique applied to four adherent mammalian cancer cells lines immobilized in a three-dimensional (3D) calcium alginate hydrogel matrix, thus mimicking in vivo tissue conditions. Cells were treated with cytostatic agent5-fluoruracil (5-FU). The cell lines used in this study were SK-N-SH, HEK293, HeLa, and MCF-7. For each cell culture, three cell population densities were chosen (50,000, 100,000, and 200,000 cells/100 μL). The aim of this study was the extraction of mean impedance values at various frequencies for the assessment of the different behavior of various cancer cells when 5-FU was applied. For comparison purposes, impedance measurements were implemented on untreated immobilized cell lines. The results demonstrated not only the dependence of each cell line impedance value on the frequency, but also the relation of the impedance level to the cell population density for every individual cell line. By establishing a cell line-specific bioelectrical behavior, it is possible to obtain a unique fingerprint for each cancer cell line reaction to a selected anticancer agent.

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Single Cell Array Impedance Analysis for Cell Detection and Classification in a Microfluidic Device

Cited by 2 publications

References 8 publications

On‐chip label‐free impedance‐based detection of antibiotic permeation

On‐chip label‐free impedance‐based detection of antibiotic permeation

Bioelectrical Analysis of Various Cancer Cell Types Immobilized in 3D Matrix and Cultured in 3D-Printed Well

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