Single capripoxvirus recombinant vaccine for the protection of cattle against rinderpest and lumpy skin disease

Romero, Carlos H.; Barrett, Thomas; Evans, Slayton A.; Kitching, R.; Gershon, Paul D.; Bostock, Christopher J.; Black, D. N.

doi:10.1016/0264-410x(93)90258-y

Cited by 82 publications

(61 citation statements)

References 22 publications

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“…The common immunogenic properties of these viruses have been used for the preparation of live attenuated vaccines that protect all ruminants against CaPV infection (Kitching et al, 1987). Recombinant CaPVs have also been developed for multivalent vaccination purposes (Berhe et al, 2003;Perrin et al, 2007;Romero et al, 1993;Wade-Evans et al, 1996;Wallace et al, 2006). However, although they are antigenically closely related, restriction enzyme pattern analysis, cross-hybridization studies and, more recently, nucleic acid sequencing have shown that nearly all CaPVs can be grouped according to their host origins Cao et al, 1995;Gershon & Black, 1988;Kitching et al, 1989;Tulman et al, 2002).…”

mentioning

confidence: 99%

Capripoxvirus G-protein-coupled chemokine receptor: a host-range gene suitable for virus animal origin discrimination

Goff

Lamien

Fakhfakh

et al. 2009

Journal of General Virology

140

142

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The genus Capripoxvirus within the family Poxviridae comprises three closely related viruses, namely goat pox, sheep pox and lumpy skin disease viruses. This nomenclature is based on the animal species from which the virus was first isolated, respectively, goat, sheep and cattle. Since capripoxviruses are serologically identical, their specific identification relies exclusively on the use of molecular tools. We describe here the suitability of the G-protein-coupled chemokine receptor (GPCR) gene for use in host-range grouping of capripoxviruses. The analysis of 58 capripoxviruses showed three tight genetic clusters consisting of goat pox, sheep pox and lumpy skin disease viruses. However, a few discrepancies exist with the classical virus-host origin nomenclature: a virus isolated from sheep is grouped in the goat poxvirus clade and vice versa. Intra-group diversity was further observed for the goat pox and lumpy skin disease virus isolates. Despite the presence of nine vaccine strains, no genetic determinants of virulence were identified on the GPCR gene. For sheep poxviruses, the addition or deletion of 21 nucleic acids (7 aa) was consistently observed in the 59 terminal part of the gene. Specific signatures for each cluster were also identified. Prediction of the capripoxvirus GPCR topology, and its comparison with other known mammalian GPCRs and viral homologues, revealed not only a classical GPCR profile in the last three-quarters of the protein but also unique features such as a longer N-terminal end with a proximal hydrophobic a-helix and a shorter serine-rich C-tail.3These authors contributed equally to this work.Two supplementary tables are available with the online version of this paper.Journal of General Virology (2009), 90, 1967-1977 DOI 10.1099 Davies, 1981Davies, , 1982Diallo & Viljoen, 2007). CaPV infections lead to similar clinical signs in sheep and goats, mainly characterized by fever, excess salivation, conjunctivitis and rhinitis with ocular and nasal discharges. These symptoms are followed by eruption of pox lesions throughout the skin. LSD is a subacute to acute cattle disease with appearance of skin nodules. During epizootics of CaPVs in domestic animals, disease in wild ungulates has never been reported (Davies, 1991), although serology and isolated cases suggests that wildlife species have a possible role in virus maintenance.CaPVs are generally considered to be host-specific, leading to outbreaks in one preferred host. This is partially true since some SPPV and GTPV isolates are capable of causing severe diseases in both sheep and goats (Davies, 1976;Kitching et al., 1989). An in-depth epidemiological investigation to specifically identify the viruses involved in acute small ruminant pox diseases cannot be achieved by serological testing alone due to the very close antigenic relationship between CaPVs, with the existence of only one serotype (Kitching et al., , 1989. The common immunogenic properties of these viruses have been used for the preparation of live attenuated vaccines that ...

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confidence: 99%

Capripoxvirus G-protein-coupled chemokine receptor: a host-range gene suitable for virus animal origin discrimination

Goff

Lamien

Fakhfakh

et al. 2009

Journal of General Virology

140

142

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“…Therefore it would make a more suitable viral vector for expressing immunogenic antigens of veterinary importance in their species. Romero et al [94][95][96][97] reported the constructions of recombinant CPV, containing the fusion (F) or haemagglutinin (H) gene of rinderpest virus, and their vaccine efficacy in goats or cattle against rinderpest, peste des petits, or lumpy skin diseases. Wade-Evans et al [121] constructed a recombinant CPV expressing the major core protein VP7 of bluetongue virus (BTV).…”

Section: ) Capripox Virusesmentioning

confidence: 99%

Recombinant Viral Vector Vaccines for the Veterinary Use.

Yokoyama

Maeda

Mikami

1997

The Journal of Veterinary Medical Science

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ABSTRACT. Recently, genetically engineering using recombinant DNA techniques has been applied to design new viral vaccines in order to reduce some problems which present viral vaccines have. Up to now, many viruses have been investigated for development of recombinant attenuated vaccines or live viral vectors for delivery of foreign immunogenic antigens. In this review, we introduced three kind of viruses; herpesviruses, vaccinia viruses, and adenoviruses, which have best widely been studied as recombinant vaccines or delivery vaccines for the veterinary use. -KEY WORDS: recombinant polyvalent vaccine, recombinant vaccine, viral vector.

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“…As for other members of the Poxviridae family, attenuated vaccine strains of LSDV are being developed as vectors for recombinant vaccines for use in the veterinary field. The Kenya Sheep-1 strain, KS-1 ( [Kitching et al, 1987] and [Kitching et al, 1989]), has been utilised for the development of recombinant rinderpest, bluetongue and peste-des-petits-ruminants virus vaccines ( [Romero et al, 1993], [Romero et al, 1994a], [Romero et al, 1994b], [Romero et al, 1994c], [WadeopenUP (December 2007) Evans et al., 1996], [Ngichabe et al, 1997] and [Berhe et al, 2003]). The southern African LSDV vaccine strain, SA-Neethling, was developed from a virulent field isolate, and is a highly host-restricted vaccine that offers long-term protection (Weiss, 1968).…”

Section: Introductionmentioning

confidence: 99%

“…However, the TK-negative selection approach failed to yield stable LSDV recombinants due to a later finding that the SA-Neethling vaccine strain of LSDV has a dependence for growth on some source of TK activity (either of viral or cellular origin) (Wallace and Viljoen, 2002). The selection strategy was then modified to that of dominant positive selection ( [Falkner and Moss, 1990] and [Romero et al, 1993]) with inclusion of the E. coli guanine phosphoribosyl transferase (gpt) gene. Following this protocol stable TK-disrupted recombinants expressing lacZ could be generated and isolated.…”

Section: Introductionmentioning

confidence: 99%

“…This paper describes the modifications made to the method of Romero et al (1993) for the generation and selection of LSDV-vectored recombinants, with additional steps included to ensure homogeneity. The substitution of lacZ with EGFP (originally isolated from the jellyfish, Aequorea victoria) (Cheng et al, 1996) as a more convenient in vivo visual selectable marker is also described.…”

Section: Introductionmentioning

confidence: 99%

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Improved method for the generation and selection of homogeneous lumpy skin disease virus (SA-Neethling) recombinants

Wallace

Weyer

Nel

et al. 2007

Journal of Virological Methods

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Lumpy skin disease virus (LSDV) is being developed as a vector for recombinant vaccines against diseases of veterinary importance. A strategy for generating viral thymidine kinase (TK) gene-disrupted recombinants which are stable and homogeneous using the South African Neethling vaccine strain of LSDV as vector has been developed.To assist with the selection process, the Escherichia coli β-galactosidase (lacZ) visual marker gene was incorporated into the constructs. However, the use of lacZ has certain limitations. An improved strategy was then devised substituting lacZ with the enhanced green fluorescent protein (EGFP) under control of the vaccinia virus (VV) P11K late promoter. The EGFP marker was found to enhance the selection process, and with the inclusion of additional sonication and filtration steps the number of passages required to select recombinants to homogeneity has been reduced. In support of the improved method for generation and selection of recombinants described, three different LSDV openUP recombinants expressing the glycoprotein genes of bovine ephemeral fever virus, RiftValley fever virus and rabies virus were prepared and characterised.

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Single capripoxvirus recombinant vaccine for the protection of cattle against rinderpest and lumpy skin disease

Cited by 82 publications

References 22 publications

Capripoxvirus G-protein-coupled chemokine receptor: a host-range gene suitable for virus animal origin discrimination

Capripoxvirus G-protein-coupled chemokine receptor: a host-range gene suitable for virus animal origin discrimination

Recombinant Viral Vector Vaccines for the Veterinary Use.

Improved method for the generation and selection of homogeneous lumpy skin disease virus (SA-Neethling) recombinants

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