Single and repeated oral dose toxicity study of fucoxanthin (FX), a marine carotenoid, in mice

Beppu, Fumiaki; Niwano, Yoshimi; Tsukui, Takayuki; Hosokawa, Masashi; Miyashita, Kazuo

doi:10.2131/jts.34.501

Cited by 151 publications

(102 citation statements)

References 24 publications

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“…The total protein, albumin, globulin, glucose, blood urea nitrogen (BUN), alanine transaminase (ALT), aspartate amino transferase (AST), P, Ca, and Mg were measured by Eone Laboratories (Incheon, Korea). For histopathology, the organs of 405 mg/kg Mg and 405 mg/kg Ca groups were fixed in 10 % neutrally buffered formalin and embedded in paraffin after 18 h. Slices of 3~4 µm thickness were prepared, and stained with hematoxylin and eosin (H&E) (Beppu et al 2009). …”

Section: Repeated-dose Oral Toxicity Testsmentioning

confidence: 99%

Single- and repeated-dose oral toxicity tests of deep sea water mineral extracts in ICR mice

Hwang

Cho

Lee

et al. 2016

JABC

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Deep sea water (DSW) is located 100 to 500 m below the sea surface. DSW is widely used in various fields, and is an important source of minerals that can be used to treat mineral deficiency. In the present study, the oral toxicity of DSW-mineral extracts was determined using single-dose and 14-day repeated dose oral toxicity tests in ICR mice. For the single-dose oral toxicity tests, mineral extracts of magnesium (Mg) and calcium (Ca) at doses of 0, 6, 270, 810, and 1,350 mg/kg, respectively, were orally administered to mice once at the beginning of the experiment, and the mice were observed for 14 days. For the 14-day repeated dose oral toxicity tests, Mg and Ca mineral extracts at doses of 0, 3, 135, 405, 675 mg/kg, respectively, were orally administered to mice daily, and the mice were observed for 14 days. Various tests were performed including visual observation; analysis of relative organ weight, food intake, and organ weight; biochemical analysis, and histopathology. The results indicated that mortality and changes in appearance were not observed among differentially administered groups of male and female ICR mice during the experimental period. Differences in body weight gain, food intake, organ weight, and histopathology parameters were not significant between the control and mineral-administered groups. Some results of the biochemical analyses were significantly different, but showed no specific tendencies. Overall, no evidence of toxicity was observed from the oral administration of DSW extracts of Ca and Mg in ICR mice.

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Section: Repeated-dose Oral Toxicity Testsmentioning

confidence: 99%

Single- and repeated-dose oral toxicity tests of deep sea water mineral extracts in ICR mice

Hwang

Cho

Lee

et al. 2016

JABC

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“…Secara in vivo, fukosantin yang diberikan secara oral selama 28 hari juga tidak menunjukkan gejala toksik pada tikus (Kadekaru, Toyama & Yasumoto, 2008). Pemberian fukosantin secara oral pada dosis 500 dan 1000 mg/kg bb tidak menyebabkan toksisitas dan abnormalitas ginjal dan hati pada mencit (Beppu, Niwano, Tsukui, Hosokawa, & Miyashita, 2009). Dengan sifat antikankernya yang cukup baik dan rendahnya toksisitas fukosantin terhadap sel normal, serta melimpahnya bahan baku P.australis di Indonesia, maka fukosantin memiliki prospek yang baik untuk dikembangkan sebagai agen terapi antikanker khususnya kanker payudara.…”

Section: Uji Sitotoksisitasunclassified

Isolasi Fukosantin dari Rumput Laut Coklat Padina australis dan Sitotoksisitasnya terhadap Sel MCF7 dan Sel Vero

Nursid

Noviendri

Rahayu

et al. 2017

JPBKP

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ABSTRAKFukosantin merupakan karotenoid laut yang banyak terdapat pada rumput laut coklat. Fukosantin memiliki potensi yang menjanjikan untuk diaplikasikan pada bidang kesehatan. Padina australis merupakan salah satu rumput laut coklat yang banyak mengandung fukosantin. Penelitian ini bertujuan untuk mengisolasi fukosantin dari rumput laut P. australis dan mengetahui sitotoksisitasnya terhadap sel lestari kanker MCF7 dan sel normal Vero. Isolasi fukosantin dilakukan dengan menggunakan kromatografi kolom SiO 2 . Identifikasi fukosantin dilakukan dengan menggunakan liquid chromatography ion trap time of flight mass spectrofotometer (LC-IT-ToF-MS) berdasarkan profil serapan UV dan berat molekul. Uj i sitotoksik dilakukan dengan menggunakan uji (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (uji MTT). Fukosantin berhasil diisolasi dari ekstrak kasar metanol. Fukosantin terkonfirmasi pada panjang gelombang maksimum (maks) 447 nm pada spektrum UV. Puncak monoisotopik ion molekul fukosantin terdeteksi pada nilai m/z 659,3612 [M+H]. Hasil uji MTT memperlihatkan bahwa fukosantin dari P. australis memiliki sitotoksisitas terhadap sel MCF7 dengan nilai IC 50 sebesar 34,7 µg/ml dan relatif tidak toksik terhadap sel normal Vero dengan nilai IC 50 sebesar 1071,6 µg/ml. KATA KUNCI: rumput laut coklat, Padina australis, fukosantin, sitotoksisitas ABSTRACT Fucoxanthin is marine carotenoid that present in brown algae. It has considerable potential and promising application in human health. Padina australis is one of brown algae containing fucoxanthin. The aims of this research was to isolate fucoxanthin from P. australis and to investigate cytotoxicity against MCF7 cancer cell and Vero normal cell line. Fucoxanthin isolation was performed by using SiO 2 column chromatography. Fucoxanthin identification was done by using liquid chromatography ion trap time of flight mass spectrofotometer (LC-IT-ToF-MS) based on UV absorbance profile and molecular weight. Cytotoxicity test was evaluated by using

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“…Fucoxanthin toxicity has arisen as a concern from results of mice experiments 37,38 . Fucoxanthin was orally administered at doses of 500 and 1,000 mg/kg to male and female ICR mice for 30 days.…”

Section: Fucoxanthin Absorption and Safetymentioning

confidence: 99%

Nutraceutical Effects of Fucoxanthin for Obesity and Diabetes Therapy: A Review

Maeda

2015

J. Oleo Sci.

103

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Single and repeated oral dose toxicity study of fucoxanthin (FX), a marine carotenoid, in mice

Cited by 151 publications

References 24 publications

Single- and repeated-dose oral toxicity tests of deep sea water mineral extracts in ICR mice

Single- and repeated-dose oral toxicity tests of deep sea water mineral extracts in ICR mice

Isolasi Fukosantin dari Rumput Laut Coklat Padina australis dan Sitotoksisitasnya terhadap Sel MCF7 dan Sel Vero

Nutraceutical Effects of Fucoxanthin for Obesity and Diabetes Therapy: A Review

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