Sinapic Acid Affects Phenolic and Trichothecene Profiles of F. culmorum and F. graminearum Sensu Stricto

Kulik, Tomasz; Stuper‐Szablewska, Kinga; Bilska, Katarzyna; Buśko, Maciej; Ostrowska-Kołodziejczak, Anna; Załuski, Dariusz; Perkowski, J.

doi:10.3390/toxins9090264

Cited by 19 publications

(20 citation statements)

References 46 publications

(72 reference statements)

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“…In this study, it was found for the first time that flavonoids display variable effect on fungal secondary metabolic profiles. The observed high variability is in line with previous reports demonstrating variable effects of other plant resistance related compounds such as phenolic acids [ 44 , 45 ] and lignans [ 60 ] on this group of toxigenic fungi. Notably, the in vitro results presented in this paper are in line with recent in planta studies demonstrating that resistance to Fusarium diseases is closely associated with the promoted biosynthesis of phenylpropanoids, including flavonoids [ 33 , 43 , 61 , 62 ].…”

Section: Resultssupporting

confidence: 91%

“…Previous in vitro studies showed that different strains of F. culmorum and F. graminearum s.s. accumulate phenolic acids in the media [ 44 , 45 ]. In this study, we screened fungi for flavonoid production.…”

Section: Resultsmentioning

confidence: 99%

“…The analysis also included the Tri10 gene responsible for regulation of multiple Tri genes [ 58 ]. Gene expression analyses were performed as previously described in Kulik et al [ 44 , 45 ]. The Ct values of the target Tri4 , Tri5 , Tri10 and reference Ef1α gene were compared in the control and treated samples and normalized relative to the Ct values obtained for the reference EF1α gene using the REST 2009 software [ 69 ].…”

Section: Methodsmentioning

confidence: 99%

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Changes in Phenylpropanoid and Trichothecene Production by Fusarium culmorum and F. graminearum Sensu Stricto via Exposure to Flavonoids

Bilska

Stuper‐Szablewska

Kulik

et al. 2018

Toxins

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Flavonoids are a group of hydroxylated polyphenolic compounds widely distributed in the plant kingdom. Biosynthesis of these compounds involves type III PKSs, whose presence has been recently predicted in some fungal species through genome sequencing efforts. In this study, for the first time it was found that Fusaria produce flavonoids on solid YES medium. Naringenin, as the central precursor of all flavonoids, was produced at highest quantities, followed by quercetin, kaempferol, apigenin and luteolin. In plants, flavonoids are involved in the protection of cereals to a wide range of stresses, including host defense against Fusaria. Under in vitro conditions, strains of Fusarium culmorum and F. graminearum sensu stricto were incubated at levels of flavonoids close to amounts produced by cereals in response to fungal infection. The amounts of exogenous naringenin, apigenin, luteolin, kaempferol and quercetin were reduced and converted by fungi to the other flavonoid derivatives. Treatment of fungi with naringenin derivatives led to the inhibition of naringenin production. Correspondingly, the production of fungal-derived phenolic acids decreased in flavonoid treated samples, although this effect appeared to be dependent on the strain, flavonoid molecule and its concentration. Fusaria showed high variability in trichothecene production in response to flavonoids. With emphasis on quercetin, mycotoxin accumulation in the media was significantly decreased by luteolin, kaempferol, naringenin and apigenin. However, in some cases, apigenin led to the increase of mycotoxin content in the media. Gene expression experiments of Tri genes responsible for trichothecene biosynthesis (Tri4, Tri5 and Tri10) proved that the inhibition of mycotoxin production by flavonoids occurred at the transcriptional level. However, the changes in Tri transcript levels were not significant in most apigenin and all kaempferol-treated cultures. In this study, a link was established between antioxidant and antiradical properties of flavonoids and their effects on fungi.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Changes in Phenylpropanoid and Trichothecene Production by Fusarium culmorum and F. graminearum Sensu Stricto via Exposure to Flavonoids

Bilska

Stuper‐Szablewska

Kulik

et al. 2018

Toxins

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“…However, in our study, this effect was only observed for FP, but not for FG and FL. This indicates that FP is not as sensitive to p-hydroxybenzoic acid as FG and FL which could be due to a transformation of the acid into a less affecting substance, as observed by Kulik et al (2017). Also, a potential interference with trichothecene biosynthesis at the transcriptional level (Pani et al 2016) which occurs in FG and FL, but not in FP, should be investigated.…”

Section: Discussionmentioning

confidence: 98%

Can plant phenolic compounds reduceFusariumgrowth and mycotoxin production in cereals?

Schöneberg

Kibler

Sulyok

et al. 2018

Food Additives & Contaminants: Part A

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To assess the in vitro activity of three phenolic acids (ferulic acid, p-hydroxybenzoic acid, vanillic acid) and two flavonols (quercetin, rutin) on mycelial growth and mycotoxin accumulation of Fusarium graminearum (FG), F. langsethiae (FL) and F. poae (FP), two different approaches were chosen. First, grains from oat varieties were inoculated with a suspension of three FL isolates to determine the influence of phenolic compounds on the accumulation of mycotoxins. The oat variety Zorro showed a tendency for lower accumulation of T-2/HT-2, diacetoxyscirpenol and neosolaniol. Second, a mycelium growth assay was conducted to follow FG, FL and FP growth on cereal based media supplemented with phenolic compounds. Increasing concentrations of ferulic acid substantially inhibited growth of FG and FL, while FP growth was reduced to 57%. In contrast, p-hydroxybenzoic acid, vanillic acid, quercetin, and rutin slightly stimulated mycelium growth. Results about mycotoxin production in cereal based media were less conclusive.

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“…Sinapic acid have been shown to affect the production of trichothecenes by F. culmorum and F. graminearum, which also respond by stimulation of ergosterol. This effect might be indirect since treatment with sinapic acid led to dramatic accumulation of its biosynthetic precursor ferulic acid, and chlorogenic acid biosynthesis [59]. Both compounds have caffeic acid as a common precursor, and an increased accumulation of caffeic acid may occur in the early incubation period of fungi in the presence of sinapic acid [59].…”

Section: Discussionmentioning

confidence: 99%

Methanolic Extracts from Cultivated Mushrooms Affect the Production of Fumonisins B and Fusaric Acid by Fusarium verticillioides

Merel

Savoie

Mata

et al. 2020

Toxins

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The maize pathogen Fusarium verticillioides and their mycotoxins cause damage to plants, animals, and human health. This work aimed to evaluate the effect of crude extracts (CEs) from Agaricus subrufescens, Lentinula edodes, and Pleurotus ostreatus fruiting bodies on in vitro production of biomass and mycotoxins by two strains of F. verticillioides. Stipes and pilei were separated before extraction for A. subrufescens and L. edodes. Comparative metabolomics and dereplication of phenolic compounds were used to analyze all CEs. Mushroom CEs did not significantly inhibit the production of mycelial biomass at concentrations of 2 mg mL−1. CEs from A. subrufescens (stipes and pilei) and L. edodes pilei inhibited the production of fumonisins B1 + B2 + B3 by 54% to 80%, whereas CE from P. ostreatus had no effect. In contrast, CE from L. edodes stipes dramatically increased the concentration of fumonisins in culture media. Fusaric acid concentration was decreased in cultures by all CEs except L. edodes stipes. Differences in phenolic composition of the extracts may explain the different effects of the CE treatments on the production of mycotoxins. The opposing activities of stipes and pilei from L. edodes offer an opportunity to search for active compounds to control the mycotoxin production by F. verticillioides.

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Sinapic Acid Affects Phenolic and Trichothecene Profiles of F. culmorum and F. graminearum Sensu Stricto

Cited by 19 publications

References 46 publications

Changes in Phenylpropanoid and Trichothecene Production by Fusarium culmorum and F. graminearum Sensu Stricto via Exposure to Flavonoids

Changes in Phenylpropanoid and Trichothecene Production by Fusarium culmorum and F. graminearum Sensu Stricto via Exposure to Flavonoids

Can plant phenolic compounds reduceFusariumgrowth and mycotoxin production in cereals?

Methanolic Extracts from Cultivated Mushrooms Affect the Production of Fumonisins B and Fusaric Acid by Fusarium verticillioides

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