Simultaneous use of oxalate-degrading bacteria and herbal extract to reduce the urinary oxalate in a rat model: A new strategy

Afkari, Rouhi; Feizabadi, Mohammad Mehdi; Ansari-Moghaddam, Alireza; Safari, Tahereh; Bokaeian, Mohammad

doi:10.1590/s1677-5538.ibju.2019.0167

Cited by 14 publications

(7 citation statements)

References 27 publications

(45 reference statements)

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“…The absence of correlation between weight loss and 24-hours urinary parameters of calcium oxalate stone formers suggests that factors other than obesity are implicated in the physiology of calcium oxalate stone formation. Afkari et al suggest that probiotic bacteria may restore gut microbiota balance and reduce urinary oxalate excretion (24). Our study demonstrated that weight is associated with urinary oxalate excretion.…”

Section: Discussionsupporting

confidence: 59%

Effect of a low-calorie diet on 24-hour urinary parameters of obese adults with idiopathic calcium oxalate kidney stones

et al. 2021

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Purpose: to evaluate the effect of low-calorie diet on 24-hour urinary metabolic parameters of obese adults with idiopathic calcium oxalate kidney stones. Materials and Methods: Adult idiopathic calcium oxalate stone formers, with body mass index (BMI) ≥30kg/m 2 and a known lithogenic metabolic abnormality, were submitted to low-calorie diet for twelve weeks. After enrolment, anthropometric measures, serum exams, 24-hour urinary metabolic parameters and body impedance were collected one month prior to dietary intervention and at the end of twelve weeks. Correlations between weight loss, waist circumference loss, fat loss and variation in 24-hour urinary lithogenic parameters and calcium oxalate urinary supersaturation (CaOx SS) as per Tiselius equation were analysed. Results: From January 2017 to January 2018, 39 patients were enrolled to participate in this study. Median (range) prescribed diet was 1300 (1100-2100) Kcal/day. Mean age was 51.7±11.0 (29-68) years old and 69.2% were female. 30.8% of the participants shifted from obesity to BMI <30kg/m 2 and none to BMI <25kg/m 2 . A significant correlation was found between baseline 24-hour urinary oxalate and weight (p=0.018) and BMI (p=0.026). No correlation was found between variation of weight, waist circumference, fat mass and 24-h urinary stone risk factors or CaOx SS. Conclusions: Short-term modest weight loss induced by twelve weeks of low-calorie diet is not associated with a decrease of 24-hour urinary lithogenic parameters in idiopathic calcium oxalate stone formers. Calcium oxalate urinary stone formation is probably multifactorial and driven by other factors than weight.

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Section: Discussionsupporting

confidence: 59%

Effect of a low-calorie diet on 24-hour urinary parameters of obese adults with idiopathic calcium oxalate kidney stones

et al. 2021

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“…Sample size consideration was based on previous reports on a similar topic [20,21] and calculated using G*Power Software, version 3.1.9.4. The authors have reported the effect sizes from 0.93 to 2.56 with sample sizes ranged between 6 and 8 animals in each group.…”

Section: Sample Sizementioning

confidence: 99%

Synbiotic supplementation and oxalate homeostasis in rats: focus on microbiota oxalate-degrading activity

et al. 2022

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Background The present study aimed (i) to evaluate whether ceftriaxone treatment could affect not only intestinal oxalate-degrading bacteria number but their total activity to degrade oxalate and in uence oxalate homeostasis in rats, (ii) to test the effect of commercially available probiotics and a synbiotic on total fecal oxalate-degrading activity, (iii) and to estimate the ability of synbiotic to restore fecal oxalatedegrading activity and ceftriaxone-induced disruption of oxalate homeostasis in rats.Methods Twenty-eight female Wistar rats (200-300 g) were randomly divided into 4 groups (n = 7). Group 1 was treated with vehicle sterile water (0.1 ml, i.m., 14 days); Group 2 received synbiotic (30 mg/kg, per os, 14 days); Group 3 was treated with ceftriaxone (300 mg/kg, i.m., 7 days); Group 4 was supplemented with ceftriaxone and synbiotic. Oxalate-degrading bacteria number and their total activity, urinary and plasma oxalate concentrations were measured on days 1 and 57 after the treatment withdrawal.Results Ceftriaxone treatment reduced total fecal oxalate-degrading activity independently on oxalatedegrading bacteria number and increased urinary and plasma oxalate concentrations. The synbiotic had a high oxalate-degrading activity vs probiotics and was able to restore fecal oxalate-degrading activity and signi cantly decrease urinary oxalate excretion in antibiotic-treated rats.Conclusion Total fecal oxalate-degrading activity but not oxalate-degrading bacteria number should be thoroughly examined in the future to develop predictive diagnostics methods, targeted prevention and personalized treatment in kidney stone disease. Synbiotic supplementation had a bene cial effect on the total oxalate-degrading activity of gut microbiota, which resulted in decreased UOx excretion in rats.

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“…This can be a new therapeutic approach to prevent the hyperoxaluria. 17 The search for new probiotic strains of bacteria with high ODA is present challenge in the world as well as the development of new methods for estimation of ODA. Our study extends a number of methods for finding new effective bacterial agents to fight against urolithiasis that might have clinical application.…”

Section: Discussionmentioning

confidence: 99%

The oxalate-degrading activity of Lactobacillus spp. isolated from different sources as the potential probiotic modulators for oxalate homeostasis

Akulenko¹,

Skovorodka²,

Serhiichuk³

et al. 2020

JMEN

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Background: Currently, diseases of the urinary system are observed in 3.5–4% of the world's population. According to WHO, the number of people suffering from this pathology doubles every 7–10 years. To date, hyperoxaluria is considered as the main risk factor for the formation of oxalate-calcium stones, which account for 75% of all kidney stones. One of the main causes of hyperoxaluria is a decrease in the number of microorganisms capable of degrading oxalates, which occurs due to the disruption of the intestinal microbiota. Oxalate-degrading bacteria include the genera Lactobacillus, Bifidobacterium, Oxalobacter formigenes etc. Searching of probiotic strains with high oxalate-degrading activity have become one of the priorities from the context of research. The aim of the present study was to isolate Lactobacillus spp from different sources and to determine their ability to degrade oxalate. Methods: A total of 23 Lactobacillus spp. from food of animal and vegetable origin were isolated with selective MRS Broth medium and further cultured on MRS Agar or Oxalate Medium with 5 g/l sodium oxalate. ANAERO test23 was used to assess the species affiliation. Oxalate-degrading activity (ODA) was measured by redox titration with KMnO4. Results: Only 7 species of isolated bacteria out of 23 showed the ability to grow on the oxalate-containing culture medium. According to the morphological and physiological-biochemical characteristics, these bacteria belonged to the genus Lactobacillus: L. nagelii – 2 spp, L. rhamnosus – 2 spp., L. frumenti - 1 spp, L. plantarum – 1 spp, L. acidophilu – 1 spp. The most active metabolizers of oxalate on Oxalate Medium were L. plantarum S3 – 42%; L. acidophilus S5 – 38%, and L. nagelii Z2 – 35%; the worst results were shown by L. rhamnosus K7 and L. nagelii S12 – both metabolized only 7% of sodium oxalate. Conclusions: The redoximetric titration with KMnO4 was adopted to evaluate the ODA of bacteria in culture media. Lactobacillus spp. isolated from different sources differs according to the level of ODA. Three promising Lactobacillus species were selected for further estimation of probiotic profile.

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Simultaneous use of oxalate-degrading bacteria and herbal extract to reduce the urinary oxalate in a rat model: A new strategy

Cited by 14 publications

References 27 publications

Effect of a low-calorie diet on 24-hour urinary parameters of obese adults with idiopathic calcium oxalate kidney stones

Effect of a low-calorie diet on 24-hour urinary parameters of obese adults with idiopathic calcium oxalate kidney stones

Synbiotic supplementation and oxalate homeostasis in rats: focus on microbiota oxalate-degrading activity

The oxalate-degrading activity of Lactobacillus spp. isolated from different sources as the potential probiotic modulators for oxalate homeostasis

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