Simultaneous screening of homotaurine and taurine in marine macro-algae using liquid chromatography–fluorescence detection

Mehdinia, Ali; Rostami, Simindokht; Dadkhah, Sahar; Fumani, Neda Sheijooni

doi:10.1007/s13738-017-1150-9

Cited by 9 publications

(21 citation statements)

References 26 publications

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“…Thus, pH of 9.5 was selected for derivatization reaction. However, most of the published works for derivatization of amino acids with OPA has been done with buffer at pH 10 or 10.4 [19,21,25].…”

Section: Liquid Chromatographic and Spectrophotometric Determination mentioning

confidence: 99%

“…The sensitivity and chromatographic separation conditions for the current modified methods were compared with the previous published studies for determination of taurine after derivatization with OPA reagent. As indicated in table 5 most of used techniques for taurine determination are based on HPLC with fluorescence detection [19][20][21] which is not available in many laboratories. In present work we used universal instruments i.e.…”

Section: Comparison Of Present Work With Other Reported Studiesmentioning

confidence: 99%

“…Since taurine has no chromophore, a derivatization step is important to permit its detectability using optical detection [1,5]. Many reagents have been used for taurine derivatization such as 2,4-dinitrofluorobenzene (DNFB) [2], 2-[2-(dibenzocarbazol)-ethoxy]ethyl chloro-formate (DBCEC) [14], ninhydrine [15], Hantzsch reagent, tetracyanoethylene [12], 4-(5,6-dimethoxy-2-phthalimidinyl)-2 methoxyphenylsulfonyl chloride (DMS-Cl) [15], phenol and sodium hypochlorite [16], 4-chloro-4-nitrobenz-2oxa-1,3-diazole (NBD-Cl) [17] , fluorescamine [18], and O-phthalaldehyde (OPA) [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

“…OPA is common cheap reagent that is used for derivatization of free amino acids including taurine followed by HPLC analysis [19,21]. OPA reacts rapidly with primary amine and amino acids in presence of reducing agent (alkylthiol or sulfite group) to form isoindole ring [21][22][23]. The compound 1-alkylthio-N-alkylisoindoles which produce from the reaction of amino acids and OPA-alkylthiol reagent is unstable due to further reaction with excess OPA in the derivatization matrix.…”

Section: Introductionmentioning

confidence: 99%

“…Hence in this study, we used OPA and sodium sulfite for taurine derivatization. HPLC with fluorescence and electrochemical detection (ED) are the most frequent methods for determination of taurine after pre-column derivatization with OPA [19- 21,25]; but these detectors are not available in many laboratories. Although the OPA fluorometric method has low to nanomolar's detection limit [26], however, the concentration of taurine is high in energy drinks and thus fast analysis and cheap method is critical issue to consider.…”

Section: Introductionmentioning

confidence: 99%

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Liquid Chromatographic and Spectrophotometric Determination of Taurine in Energy Drinks Based on O-Phthalaldehyde-Sulfite Derivatization

Omer¹,

Omar²,

Abdelaziz³

et al. 2019

J Food Chem Nanotechol

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Rapid and efficient high-performance liquid chromatographic and UV-Vis spectrophotometric methods have been optimized and validated for taurine determination in energy drinks. Taurine was derivatized with o-phthalaldehyde and sodium sulfite in alkaline media prior to analysis. The optimum derivatization parameters were found to be 0.1 M borate buffer at pH 9.5, reaction time 5.0 min, o-phthalaldehyde concentration of 60 mg L-1 , sodium sulfite concentration of 202 mg L-1 and water as diluting solvent. The analytical parameters such as linearity, limit of detection (LOD), limit of quantification (LOQ), precision and accuracy were investigated. The methods were linear in range 0.5-20 mg L-1 and 0.5-15 mg L-1 with correlation coefficient (r 2) of 0.9998 and 0.9996 for HPLC-PDA and spectrophotometer respectively. The LODs were 0.109 mg L-1 and 0.141 mg L-1 for HPLC-PDA and spectrophotometer respectively. The precision (RSD%) of intra-day and inter-day of the methods were 1.816-1.278% and 2.858-2.236% respectively, for HPLC-PDA and spectrophotometer respectively. Recoveries of taurine ranging from 90% to 105%, (n = 3) were obtained. The methods were successfully applied for determination of taurine in energy drink samples.

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Section: Liquid Chromatographic and Spectrophotometric Determination mentioning

confidence: 99%

Section: Comparison Of Present Work With Other Reported Studiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Liquid Chromatographic and Spectrophotometric Determination of Taurine in Energy Drinks Based on O-Phthalaldehyde-Sulfite Derivatization

Omer¹,

Omar²,

Abdelaziz³

et al. 2019

J Food Chem Nanotechol

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A validated ultra-performance liquid chromatography with diode array detection coupled to electrospray ionization and triple quadrupole mass spectrometry method to simultaneously quantify taurine, homotaurine, hypotaurine and amino acids in macro- and microalgae

Terriente-Palacios

Dı́az

Castellari

2019

Journal of Chromatography A

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A fast and reliable method for the simultaneous quantification of Taurine, Homotaurine, Hypotaurine and 19 amino acids in algae samples by Ultra-performance liquid chromatography coupled with diode array and tandem mass spectrometry (UHPLC-DAD-MS/MS) was optimized and validated.Target compounds were chromatographically resolved in less than 15 minutes. (ESI)-MS/MS electrospray ionization and pure analytical standards were used to confirm the identity of all analytes, while quantitation was carried out with diode array detection. Validation parameters of the method were satisfactory: Resolution of peak pairs was always higher than 1.55; all analytical curves showed R 2 > 0.99, with working ranges between 0.04 mg/g to 33.1 mg/g and 9.13 mg/g to 107 mg/g and the Lack-of-fit test was not significant. The intra and inter-day precision of the method (expressed as relative standard deviation) were lower than 6 % and recovery values ranged between 95 % and 105 %. The method was demonstrated to be robust to small deliberate variations of seven variables such sample weight, volume of hydrolysis reagent, hydrolysis time and temperature, derivatization time, column temperature and flow rate.The mean expanded uncertainty for all the target compounds were 0.7 mg/g with a coverage factor of 2.Method Limits of detection and quantification varied from 0.005 * 10 -3 mg/g to 0.11 * 10 -3 mg/g and 0.01* 10 -3 mg/g to 0.22 * 10 -3 mg/g respectively, allowing the routine determination of these bioactive compounds in algae extracts.Therefore, the method was successfully applied for the quantitative determination of the 22 target compounds in five seaweed commercial samples.Relevant compounds were quantified for the first time in the five algae species, namely: i) Taurine in Gracilaria longissima and Chlorella spp., ii) Gamma-aminobutyric acid in G. longissima and L. japonica, iii) Hydroxyproline in G. longissima, Ulva lactuca, Porphyra spp., and L. japonica and iv) Homotaurine and Hypotaurine in the five species studied. Highlights• Simultaneous analysis of Taurine, Homotaurine, Hypotaurine and 19 amino acids • The method was validated and applied for the analysis of algae extracts • Quantitation was performed with DAD to support routine analyses • Homotaurine and Hypotaurine were quantified for the first time in commercial samples Keywords Homotaurine, Hypotaurine, Taurine, amino acids, Algae, UPLC-DAD-MS/MS * u(RSD R ) = standard uncertainty for within lab reproducibility ** u(rec) = standard uncertainty for recovery *** u (Pse) = Expanded uncertainty

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Levels of taurine, hypotaurine and homotaurine, and amino acids profiles in selected commercial seaweeds, microalgae, and algae-enriched food products

Terriente-Palacios

Castellari

2022

Food Chemistry

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Simultaneous screening of homotaurine and taurine in marine macro-algae using liquid chromatography–fluorescence detection

Cited by 9 publications

References 26 publications

Liquid Chromatographic and Spectrophotometric Determination of Taurine in Energy Drinks Based on O-Phthalaldehyde-Sulfite Derivatization

Liquid Chromatographic and Spectrophotometric Determination of Taurine in Energy Drinks Based on O-Phthalaldehyde-Sulfite Derivatization

A validated ultra-performance liquid chromatography with diode array detection coupled to electrospray ionization and triple quadrupole mass spectrometry method to simultaneously quantify taurine, homotaurine, hypotaurine and amino acids in macro- and microalgae

Levels of taurine, hypotaurine and homotaurine, and amino acids profiles in selected commercial seaweeds, microalgae, and algae-enriched food products

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