Simultaneous Quantification of Multiple Representative Components in the Xian-Ling-Gu-Bao Capsule by Ultra-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry

Yao, Zhihong; Qin, Z. H.; Hong, Chen; Wu, Xiaomeng; Dai, Yi; Wang, Xinluan; Qin, Ling; Ye, Wen‐Cai; Yao, Xin‐Sheng

doi:10.3390/molecules22060927

Cited by 13 publications

(6 citation statements)

References 25 publications

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“…The effective constituents obtained from AF are complex and various, according to a previous qualitative study. For the selection of quantitative chemical markers, four main principles are followed [ 45 ]. First, they are components absorbed in vivo.…”

Section: Resultsmentioning

confidence: 99%

Chemical Profiles and Simultaneous Quantification of Aurantii fructus by Use of HPLC-Q-TOF-MS Combined with GC-MS and HPLC Methods

Wang

et al. 2018

Molecules

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Aurantii fructus (AF) is a traditional Chinese medicine that has been used to improve gastrointestinal motility disorders for over a thousand years, but there is no exhaustive identification of the basic chemical components and comprehensive quality control of this herb. In this study, high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS) and gas chromatography coupled mass spectrometry (GC-MS) were employed to identify the basic chemical compounds, and high-performance liquid chromatography (HPLC) was developed to determine the major biochemical markers from AF extract. There were 104 compounds belonging to eight structure types, including 13 amino acids or peptides, seven alkaloids, 18 flavanones, 14 flavones, 15 polymethoxyflavonoids, six triterpenoids, nine coumarins, and 18 volatile oils, as well as four other compounds that were systematically identified as the basic components from AF, and among them, 41 compounds were reported for the first time. Twelve bioactive ingredients were chosen as the benchmark markers to evaluate the quality of AF. The analysis was completed with a gradient elution at a flow rate of 0.7 mL/min within 55 min. This efficient method was validated showing good linearity, precision, stability, repeatability and recovery. Furthermore, the method was successfully applied to the simultaneous determination of 12 chemical markers in different samples of AF. This study could be applied to the identification of multiple bioactive substances and improve the quality control of AF.

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Section: Resultsmentioning

confidence: 99%

Chemical Profiles and Simultaneous Quantification of Aurantii fructus by Use of HPLC-Q-TOF-MS Combined with GC-MS and HPLC Methods

Wang

et al. 2018

Molecules

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“…MGN isolated from Epimedii herba in concentration 1.54-30.74 µg/mL is the component of the Xian-Ling-Gu-Bao (XLGB) capsule. XLGB capsule is an extensively used remedy in Chinese natural medicine for the treatment of osteoporosis [101]. It has been reported that XLGB shows anti-osteoporosis activity in ovariectomized mice [102,103] and rats with oral dosages at 236 mg/kg/day and 270 mg/kg/day, respectively [47].…”

Section: Antiosteoporosis Effectmentioning

confidence: 99%

Advances in Chemistry and Bioactivity of Magnoflorine and Magnoflorine-Containing Extracts

Okon

Kukuła-Koch

Jarząb

et al. 2020

IJMS

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The review collects together some recent information on the identity and pharmacological properties of magnoflorine, a quaternary aporphine alkaloid, that is widely distributed within the representatives of several botanical families like Berberidaceae, Magnoliaceae, Papaveraceae, or Menispermaceae. Several findings published in the scientific publications mention its application in the treatment of a wide spectrum of diseases including inflammatory ones, allergies, hypertension, osteoporosis, bacterial, viral and fungal infections, and some civilization diseases like cancer, obesity, diabetes, dementia, or depression. The pharmacokinetics and perspectives on its introduction to therapeutic strategies will also be discussed.

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“…However, there is limited literature available on the anabolic effect of herbal compounds in cartilage regeneration. In our previous study, after screening of 34 most abundant compounds in the herbal Fufang Xian Ling Gu Bao formula (XLGB), which has been used for treatment of osteoporosis, aseptic osteonecrosis, osteoarthritis, and bone fractures in Traditional Chinese Medicine (TCM) [53][54][55][56], we found 6 small molecules with potent anabolic and anti-inflammatory properties [57].…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory and Chondroprotective Effects of Vanillic Acid and Epimedin C in Human Osteoarthritic Chondrocytes

et al. 2020

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In osteoarthritis (OA), inhibition of excessively expressed pro-inflammatory cytokines in the OA joint and increasing the anabolism for cartilage regeneration are necessary. In this ex-vivo study, we used an inflammatory model of human OA chondrocytes microtissues, consisting of treatment with cytokines (interleukin 1β (IL-1β)/tumor necrosis factor α (TNF-α)) with or without supplementation of six herbal compounds with previously identified chondroprotective effect. The compounds were assessed for their capacity to modulate the key catabolic and anabolic factors using several molecular analyses. We selectively investigated the mechanism of action of the two most potent compounds Vanillic acid (VA) and Epimedin C (Epi C). After identification of the anti-inflammatory and anabolic properties of VA and Epi C, the Ingenuity Pathway Analysis showed that in both treatment groups, osteoarthritic signaling pathways were inhibited. In the treatment group with VA, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling was inhibited by attenuation of the nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (IκBα) phosphorylation. Epi C showed a significant anabolic effect by increasing the expression of collagenous and non-collagenous matrix proteins. In conclusion, VA, through inhibition of phosphorylation in NF-κB signaling pathway and Epi C, by increasing the expression of extracellular matrix components, showed significant anti-inflammatory and anabolic properties and might be potentially used in combination to treat or prevent joint OA.

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Simultaneous Quantification of Multiple Representative Components in the Xian-Ling-Gu-Bao Capsule by Ultra-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry

Cited by 13 publications

References 25 publications

Chemical Profiles and Simultaneous Quantification of Aurantii fructus by Use of HPLC-Q-TOF-MS Combined with GC-MS and HPLC Methods

Chemical Profiles and Simultaneous Quantification of Aurantii fructus by Use of HPLC-Q-TOF-MS Combined with GC-MS and HPLC Methods

Advances in Chemistry and Bioactivity of Magnoflorine and Magnoflorine-Containing Extracts

Anti-Inflammatory and Chondroprotective Effects of Vanillic Acid and Epimedin C in Human Osteoarthritic Chondrocytes

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